35results about How to "Improve the cumulative effect" patented technology

The invention discloses an open-type culture method of microalgae. The method includes the steps of: (1) cultivating a facultative anaerobic bacteria seed liquid; (2) cultivating an autotrophic microalgae seed liquid; and (3) inoculating the facultative anaerobic bacteria seed liquid and the autotrophic microalgae seed liquid into an open-type photobioreactor with a built-in membrane separation device, which divides the photobioreactor into an upper zone and a lower zone, to carry out semi-mixing culture, fermenting the anaerobic bacteria in the zone below the membrane separation device, and subjecting the autotrophic microalgae to photosynthesis growth in the zone above the membrane separation device, wherein the medium composition and culture conditions are the same in the two zones, and supplementing organic carbon source required by the growth and metabolism of the facultative anaerobic bacteria in the culture process. The method has the advantages of increasing the yield of autotrophic microalgae, the utilization rate of an inorganic carbon source (CO2), and oil content of the microorganisms, simplifying the culture device and improving the efficiency of large scale culture of autotrophic microalgae.

The invention discloses a method for open-type fast cultivation of oil-bearing microbes. The method comprises the following steps: (1) cultivating an oil-bearing yeast seed liquid; (2) cultivating an oil-bearing microalgae seed liquid; and (3) throwing the oil-bearing yeast seed liquid and the oil-bearing microalgae seed liquid into an open-type photobioreactor with a membrane separation device laid therein for semi-mixed cultivation, wherein the membrane separation device divides the photobioreactor into an upper area and a lower area, oil-bearing yeast is cultivated in the lower area of the membrane separation device, oil-bearing microalgae is subjected to photosynthetic growth in the upper area of the membrane separation device, the culture medium compositions and the cultivation conditions of the upper area and the lower area are the same, organic carbon sources required for growth and metabolism of the oil-bearing yeast are supplemented in the cultivation process, and reflective membranes are arranged on the inner wall of the upper area of the photobioreactor and at the upper end of the membrane separation device. The method has the advantages of improving the cell yield of the oil-bearing microbes and the utilization ratio of inorganic carbon sources (CO2), simplifying a cultivation device, increasing the oil content of the microbes and the product total oil content and the like.

The invention discloses an open-type rapid culture method of microalgae. The method includes the steps of: (1) cultivating a facultative anaerobic bacteria seed liquid; (2) cultivating an autotrophic microalgae seed liquid; and (3) inoculating the facultative anaerobic bacteria seed liquid and the autotrophic microalgae seed liquid into an open-type photobioreactor with a built-in membrane separation device, which divides the photobioreactor into an upper zone and a lower zone, to carry out semi-mixing culture, fermenting the anaerobic bacteria in the zone below the membrane separation device, and subjecting the autotrophic microalgae to photosynthesis growth in the zone above the membrane separation device, wherein the medium composition and culture conditions are the same in the two zones, and supplementing organic carbon source required by the growth and metabolism of the facultative anaerobic bacteria in the culture process. The method has the advantages of increasing the yield of autotrophic microalgae, the utilization rate of an inorganic carbon source (CO2), and oil content of the microorganisms, simplifying the culture device and improving efficiency of large scale culture of microalgae.

The invention proposes a preparation method of short-cut nitrification and denitrification granular sludge in high Do condition, which belongs to the technical field of waste water biological treatment. The short-cut nitrification and denitrification granular sludge mainly comprises bacilli and cocci and has higher nitrite depositing ability, denitrification ability and heterotroph activity so that the problem of free ammonia inhabitation caused when high NH4<+>-N selects and enriches nitrosation bacteria can be avoided and short-cut nitrification can be realized without increasing N2O release during short-cut nitrification in the high Do condition. The short-cut nitrification and denitrification granular sludge prepared by using the method has the advantages of compact granule structure and good stability, is suitable for treating the waste water with low COD (Chemical Oxygen Demand) and high NH4<+>-N, and can ensure the application of the short-cut nitrification and denitrification granular sludge in actual waste water treatment.

The invention discloses an efficient cultivation method of oleaginous microorganisms. The method includes the steps of: (1) cultivating an oleaginous yeast seed liquid; (2) cultivating an autotrophic microalgae seed liquid; and (3) inoculating the oleaginous yeast seed liquid and the autotrophic microalgae seed liquid into a closed photobioreactor with a built-in membrane separation device to carry out semi-mixing culture, culturing the oleaginous yeast in the membrane separation device, and subjecting the autotrophic microalgae to photosynthesis growth in the photobioreactor outside the membrane separation device, wherein the medium composition and culture condition are the same in the two reactors, and supplementing organic carbon source required by the growth and metabolism of the oleaginous yeast cells in the culture process. The method carries out semi-mixing culture on the autotrophic microalgae and the oleaginous yeast in the enclosed photobioreactor, and has the advantages of increasing the yield of autotrophic microalgae and oleaginous yeast cells, the utilization rate of an inorganic carbon (CO2), and oil content of the microorganisms, and simplifying the culture device.

The invention discloses a novel fluorine-containing acrylate monomer and a synthesis method and application thereof. The synthesis method comprises the following steps: (1) taking toluene-2,4-diisocyanate shown in a formula (II) as a raw material to perform first-step addition reaction with 2-hydroxyethyl methacrylate shown in a formula (III) by taking organic tin as a catalyst in a polar aproticsolvent, and reacting until the II basically disappears to obtain reaction liquid A containing an intermediate shown in a formula (IV); and (2) directly adding the reaction liquid A to 2-methoxyl-3-perfluorenoxy diol shown in a formula (V) to perform second-step addition reaction, obtaining reaction liquid B after the reaction is over, and performing solvent removal and column chromatography on the reaction liquid B to obtain the fluorine-containing (methyl) acrylate monomer shown in a formula (I). The invention provides the fluorine-containing acrylate monomer with a brand-new structure and capable of serving as a waterproof and oilproof treating agent; and the synthesis method is simple, and the fluorine-containing acrylate monomer can be prepared by a one-pot method.

The invention discloses an enclosed rapid culture method of microalgae. The method includes the steps of: (1) cultivating a facultative anaerobic bacteria seed liquid; (2) cultivating an autotrophic microalgae seed liquid; and (3) inoculating the facultative anaerobic bacteria seed liquid and the autotrophic microalgae seed liquid into a closed photobioreactor with a built-in membrane separation device to carry out semi-mixing culture, fermenting the anaerobic bacteria in the membrane separation device, and subjecting the autotrophic microalgae to photosynthesis growth in the photobioreactor outside the membrane separation device, wherein the medium composition and culture conditions are the same in the two reactors, and supplementing organic carbon source required by the growth and metabolism of the facultative anaerobic bacteria in the culture process. The photobioreactor also comprises a guide shell, the membrane separation device is arranged in the guide shell, and a reflective membrane is pasted on the outer surface of the guide shell. The method has the advantages of increasing the yield of autotrophic microalgae, the utilization rate of an inorganic carbon source (CO2), and oil content of the microorganisms, and simplifying the culture device.

The invention discloses a perfluoralkylene-containing acrylate monomer and a preparation method and application thereof. The preparation method comprises the following steps: (1) taking toluene-2,4-diisocyanate shown in a formula (II) as a raw material to perform first-step addition reaction with 2-hydroxyethyl methacrylate shown in a formula (III) by taking organic tin as a catalyst in a polar aprotic solvent, and reacting until the II basically disappears to obtain reaction liquid A of an intermediate shown in a formula (IV); and (2) directly adding the reaction liquid A to N-methyl-N-ethoxyl-p-perfluoralkenyl oxyl benzylamine shown in a formula (V) to perform second-step addition reaction, obtaining reaction liquid B after the reaction is over, and performing solvent removal and columnchromatography on the reaction liquid B to obtain the perfluoralkylene-containing acrylate monomer (I). The invention provides the perfluoralkylene-containing acrylate monomer (I) which is provided with a brand-new structure and can be used for preparing a surfactant; and the synthesis method for the substance is simple, and has less byproducts.

The invention discloses a production method for lipid through synchronous mixed culture of microbes. The method comprises the following steps: (1) culturing facultative anaerobic bacteria seed liquid; (2) culturing autotrophic microalgae seed liquid; and (3) mixing the facultative anaerobic bacteria seed liquid and the autotrophic microalgae seed liquid and carrying out synchronous mixed culture in an illuminated bioreactor, wherein during synchronous mixed culture, an organic carbon source needed in growth and metabolism of the facultative anaerobic bacteria is supplemented. The method provided by the invention has the advantages of increased harvest yield of autotrophic microalgae, an improved utilization rate of an inorganic carbon source (CO2), increased content of microbial lipid, a simplified culture apparatus and the like, and exerts small influence on the fermentation process of the facultative anaerobic bacteria, thereby realizing synchronous culture of the facultative anaerobic bacteria and the autotrophic microalgae.