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Enclosed culture method of microalgae

A closed culture, microalgae technology, applied in the biological field, can solve the problems of promoting the growth of algae and the accumulation of oil in microalgae cells

Active Publication Date: 2014-05-07
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above-mentioned prior art is to control the growth balance of algae in the water body through bacteria, not to promote the growth of algae and the accumulation of oil in microalgae cells

Method used

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  • Enclosed culture method of microalgae

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Embodiment Construction

[0019] The method for cultivating microalgae in a closed type using a membrane separation device in the present invention specifically includes the following content: before the semi-mixed culture, two kinds of microorganisms are separately cultured in seed liquid, and a stirring bioreactor is used to cultivate the seed liquid of facultative anaerobic bacteria, Autotrophic microalgae seed liquid cultivation is carried out in a photobioreactor. The stirred bioreactor includes facultative anaerobic bacteria and anaerobic bacteria fermentation medium. The photobioreactor includes microalgae and self-supporting SE medium . After the seed solution is qualified, it is transferred to the built-in membrane separation device for cultivating facultative anaerobic bacteria and the closed photobioreactor for cultivating microalgae cells through their respective inoculation lines, and the aseptically treated mixed medium is added for nutrition. Components and acid-base neutralizers are adj...

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Abstract

The invention discloses an enclosed culture method of microalgae. The method includes the steps of: (1) cultivating a facultative anaerobic bacteria seed liquid; (2) cultivating an autotrophic microalgae seed liquid; and (3) inoculating the facultative anaerobic bacteria seed liquid and the autotrophic microalgae seed liquid into a closed photobioreactor with a built-in membrane separation device to carry out semi-mixing culture, fermenting the anaerobic bacteria in the membrane separation device, and subjecting the autotrophic microalgae to photosynthesis growth in the photobioreactor outside the membrane separation device, wherein the medium composition and culture conditions are the same in the two reactors, and supplementing organic carbon source required by the growth and metabolism of the facultative anaerobic bacteria in the culture process. The method has the advantages of increasing the yield of autotrophic microalgae, the utilization rate of an inorganic carbon source (CO2), and oil content of the microorganisms, and simplifying the culture device.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for cultivating microalgae, in particular to a closed semi-mixed culture of autotrophic oleaginous microalgae and facultative anaerobic bacteria to obtain high microalgae cell harvest, while improving A method for the lipid content of microalgal cells. Background technique [0002] With the development of the economy, the shortage of oil resources, the contradiction between supply and demand of oil products have become increasingly prominent, and the corresponding environmental pollution problems have also become more prominent. It is inevitable to develop renewable resources through multiple channels. Diesel derived from renewable resources such as microbial oil has high energy density, low sulfur content, sufficient combustion, and good lubricity. It is also renewable, environmentally friendly, easy to biodegrade, safe for storage and transportation, and good antiknock perfo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/20C12R1/89C12R1/07C12R1/145C12R1/225C12R1/22
Inventor 王领民金平廖莎吴丹李晓姝师文静张霖高大成乔凯程国香
Owner CHINA PETROLEUM & CHEM CORP
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