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Production method for lipid through synchronous mixed culture of microbes

A mixed culture and microbial technology, applied in the biological field, can solve the problem of promoting algae growth and oil accumulation in algae cells

Active Publication Date: 2014-07-30
CHINA PETROLEUM & CHEM CORP +1
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  • Description
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Problems solved by technology

The above-mentioned prior art is to control the growth balance of algae in the water body through bacteria, not to promote the growth of algae and the accumulation of oil in algal cells

Method used

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  • Production method for lipid through synchronous mixed culture of microbes

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Embodiment Construction

[0019] The method for synchronous mixed culture of microorganisms to produce oil of the present invention specifically includes the following content: using a stirred bioreactor to cultivate facultative anaerobic bacterial seed liquid, using a light-type bioreactor to carry out self-supporting microalgae seed liquid cultivation, stirring bioreactor The reactor includes facultative anaerobic bacteria and bacterial fermentation medium, and the light-type bioreactor includes microalgae species and autotrophic medium. Before the mixed culture, the two microorganisms are cultured in seed liquid separately. After the seed liquid is qualified, the two microbial seed liquids are mixed synchronously through the synchronous mixing tank, and the mixed medium nutrients and acid-base neutralizer are added to adjust to the mixture. Cultivate the desired growth conditions. The organic carbon source in the mixed medium is used for the growth of facultative anaerobic bacteria, and the CO produ...

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Abstract

The invention discloses a production method for lipid through synchronous mixed culture of microbes. The method comprises the following steps: (1) culturing facultative anaerobic bacteria seed liquid; (2) culturing autotrophic microalgae seed liquid; and (3) mixing the facultative anaerobic bacteria seed liquid and the autotrophic microalgae seed liquid and carrying out synchronous mixed culture in an illuminated bioreactor, wherein during synchronous mixed culture, an organic carbon source needed in growth and metabolism of the facultative anaerobic bacteria is supplemented. The method provided by the invention has the advantages of increased harvest yield of autotrophic microalgae, an improved utilization rate of an inorganic carbon source (CO2), increased content of microbial lipid, a simplified culture apparatus and the like, and exerts small influence on the fermentation process of the facultative anaerobic bacteria, thereby realizing synchronous culture of the facultative anaerobic bacteria and the autotrophic microalgae.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method of utilizing autotrophic oleaginous microalgae to obtain high cell yield and increase the oil content of microalgae cells. Background technique [0002] With the increasing shortage of oil resources, rising oil prices, increasingly prominent contradictions between supply and demand of oil products, and more prominent environmental pollution problems, it is inevitable to develop renewable resources through multiple channels. Diesel from microbial oil has high energy density, low sulfur content, sufficient combustion, good lubricity, etc. It also has the characteristics of renewable, easy biodegradation, safe storage and transportation, and good anti-knock performance. It can be used as a substitute for petrochemical energy. Taste. [0003] Microalgae is one of oil-containing microorganisms. Microalgae are rich in protein, polysaccharides, unsaturated fatty acids ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/20C12P7/64C12R1/01C12R1/07C12R1/145C12R1/225C12R1/22C12R1/89
Inventor 王领民金平李晓姝师文静张霖王崇辉高大成乔凯黎元生
Owner CHINA PETROLEUM & CHEM CORP
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