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Mixed culture method of microorganism containing oil

A mixed culture and microbial technology, applied in the biological field, can solve the problems of slow accumulation of microbial cells, high cost, and low oil content, and achieve the effects of enhanced mutual compatibility, reduced production costs, and increased accumulation content

Inactive Publication Date: 2013-04-17
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of cultivating oil-containing microorganisms, there are generally disadvantages such as high cost, slow accumulation of microbial cells, and low oil content.

Method used

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  • Mixed culture method of microorganism containing oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Rhodotorula viscosus and Chlorella were cultured separately in shake flasks using their respective media to obtain seed liquids with OD values ​​of 10 and 6, respectively. The cultured Rhodotorula viscosus seed solution and Chlorella seed solution were respectively inserted into 10L bioreactors according to the inoculum volume (V / V) of 10%, and mixed culture medium was added to expand the culture. The specific culture conditions are as follows: Fermentation temperature is 30°C, ventilation volume (air) is 0.4 vvm, stirring speed is 200 rpm, pH is 7.0, and the mass concentration of organic carbon source is 1.5%. When the OD value of the fermentation broth was 2.0, the two fermentation broths were transferred to a 20L photobioreactor for mixed culture. The culture conditions were the same as those of the expanded culture, and the fermentation process was completed by continuing to culture for 5 days. Collect microbial cells, measure the dry weight and oil content, and obt...

Embodiment 2

[0034] When it is detected that the OD value of the Rhodotorula viscosus fermented liquid of expanded culture is 5.0, and when the OD value of the Chlorella fermented liquid is 10.0, the two fermented liquids are transferred to a 20L photobioreactor for mixed culture, and all the other conditions are the same as in Example 1. . Collect microbial cells, measure the dry weight and oil content, and obtain the harvested oil; measure the consumption of organic carbon sources, and calculate the carbon oil conversion rate index according to the amount of organic carbon sources consumed during the cultivation process.

Embodiment 3

[0036]When it is detected that the OD value of the Rhodotorula viscosus fermented liquid of the expanded culture is 10.0, and when the OD value of the Chlorella fermented liquid is 2.0, the two fermented liquids are transferred to a 20L photobioreactor for mixed culture, and all the other conditions are the same as in Example 1. . Collect microbial cells, measure the dry weight and oil content, and obtain the harvested oil; measure the consumption of organic carbon sources, and calculate the carbon oil conversion rate index according to the amount of organic carbon sources consumed during the cultivation process.

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Abstract

The invention discloses a mixed culture method of a microorganism containing oil, comprising the following steps: inoculating a bioreactor containing mixed culture medium with a yeast seed solution containing oil and an autotrophic microalgae seed solution for secondary enlarging culture, wherein the yeast seed solution containing oil is obtained by primary culture, and the main component of the mixed culture medium is the basic culture medium needed by the yeast cells; adding inorganic salts and trace elements needed for the growth of the microalgae; adding the yeast fermentation solution containing oil and the microalgae fermentation solution into the same bioreactor for mixed culture when the OD value of the oil-containing yeast fermentation solution subjected to enlarging culture is 5-15 and the OD value of the microalgae fermentation solution is 2.0-10. The mixed culture method has the advantages that the harvest yield of microbial cells and the content of oil in the microorganism are improved, the cost is reduced and the culture device is simplified.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for obtaining high cell yield and simultaneously harvesting microbial oil by using oleaginous yeast and autotrophic oleaginous microalgae for mixed culture. [0002] Background technique [0003] With the increasing shortage of oil resources, rising oil prices, increasingly prominent contradictions between supply and demand of oil products, and more prominent environmental pollution problems, it is inevitable to develop renewable oil resources through multiple channels. Diesel from microbial oil has high energy density, low sulfur content, sufficient combustion, good lubricity, etc. It also has the characteristics of renewable, easy biodegradation, safe storage and transportation, and good anti-knock performance. It can be used as a substitute for petrochemical energy. Taste. [0004] The literature shows that under suitable conditions, the oil produced and sto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/12C12P7/64C12R1/865C12R1/645C12R1/89
Inventor 王领民金平李晓姝师文静张霖王崇辉高大成乔凯黎元生
Owner CHINA PETROLEUM & CHEM CORP
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