A method for efficiently cultivating oil-containing microorganisms
A microbial and high-efficiency technology, which is applied in the fields of increasing the oil content of microbial cells, efficiently cultivating oil-bearing microorganisms, and high microbial cell yield, can solve the problems of low oil accumulation level, different wall breaking requirements, and poor results, and achieve better results. , improve the accumulation effect, improve the effect of oil harvest
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[0022] The method for efficiently cultivating oil-containing microorganisms of the present invention specifically includes the following content: before the semi-mixed culture, two kinds of oil-containing microorganisms are separately cultured in seed liquid, a stirring bioreactor is used to cultivate oil-containing yeast seed liquid, and a photobioreactor is used for self-supporting For microalgae seed liquid cultivation, the stirred bioreactor includes oleaginous yeast and oleaginous yeast fermentation medium, and the photobioreactor includes autotrophic microalgae and autotrophic SE medium. After the seed solution is qualified, it is transferred to the built-in membrane separation device for cultivating oleaginous yeast and the closed photobioreactor for cultivating autotrophic microalgae through their respective inoculation lines, and the nutrient components of the mixed medium after aseptic treatment are added. And acid-base neutralizer to adjust to the growth conditions r...
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