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A kind of method for rapid cultivation of microalgae in closed type

A closed, microalgae technology, applied in the biological field, can solve the problems of promoting algal growth, microalgae cell oil accumulation, etc., and achieve the effects of increasing oil yield, rapid growth process, and easy harvesting.

Active Publication Date: 2016-10-26
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

The above-mentioned prior art is to control the growth balance of algae in the water body through bacteria, not to promote the growth of algae and the accumulation of oil in microalgae cells

Method used

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  • A kind of method for rapid cultivation of microalgae in closed type

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Embodiment Construction

[0021] The method for rapidly cultivating microalgae in a closed type of the present invention specifically includes the following content: before the semi-mixed culture, the two kinds of microorganisms are separately cultured in seed liquid, and a stirring bioreactor is used to cultivate the seed liquid of facultative anaerobic bacteria, and photobiological The reactor is used for autotrophic microalgae seed liquid cultivation, the stirred bioreactor includes facultative anaerobic bacteria and anaerobic bacteria fermentation medium, and the photobioreactor includes microalgae species and autotrophic SE medium. After the seed solution is qualified, it is transferred to the built-in membrane separation device for cultivating facultative anaerobic bacteria and the closed photobioreactor with reflective film for cultivating microalgae through their respective inoculation lines, and added to the aseptically treated The nutrient components of the mixed medium and the acid-base neutr...

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Abstract

The invention discloses an enclosed rapid culture method of microalgae. The method includes the steps of: (1) cultivating a facultative anaerobic bacteria seed liquid; (2) cultivating an autotrophic microalgae seed liquid; and (3) inoculating the facultative anaerobic bacteria seed liquid and the autotrophic microalgae seed liquid into a closed photobioreactor with a built-in membrane separation device to carry out semi-mixing culture, fermenting the anaerobic bacteria in the membrane separation device, and subjecting the autotrophic microalgae to photosynthesis growth in the photobioreactor outside the membrane separation device, wherein the medium composition and culture conditions are the same in the two reactors, and supplementing organic carbon source required by the growth and metabolism of the facultative anaerobic bacteria in the culture process. The photobioreactor also comprises a guide shell, the membrane separation device is arranged in the guide shell, and a reflective membrane is pasted on the outer surface of the guide shell. The method has the advantages of increasing the yield of autotrophic microalgae, the utilization rate of an inorganic carbon source (CO2), and oil content of the microorganisms, and simplifying the culture device.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for cultivating microalgae, in particular to a closed semi-mixed rapid culture of autotrophic oleaginous microalgae and facultative anaerobic bacteria to obtain high microalgae cell harvest, and at the same time A method for increasing the oil content of microalgae cells. Background technique [0002] With the development of society and economy, the supply of fossil resources is becoming increasingly scarce, the contradiction between supply and demand of oil products is becoming more and more prominent, and the corresponding environmental pollution problems are also becoming more prominent. It is inevitable to develop renewable resources through multiple channels. Biodiesel derived from renewable resources such as microbial oil has high energy density, low sulfur content, sufficient combustion, and good lubricity. It is also renewable, environmentally friendly, easy to biodegr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/20C12R1/89C12R1/07C12R1/145C12R1/225C12R1/22
Inventor 王领民金平廖莎吴丹李晓姝师文静张霖高大成乔凯程国香
Owner CHINA PETROLEUM & CHEM CORP
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