Rapid cultivation method of oleaginous microorganism

Abstract

The invention discloses a rapid cultivation method of oleaginous microorganisms. The method includes the steps of: (1) cultivating an oleaginous yeast seed liquid; (2) cultivating an autotrophic microalgae seed liquid; and (3) inoculating the oleaginous yeast seed liquid and the autotrophic microalgae seed liquid into a closed photobioreactor with a built-in membrane separation device to carry out semi-mixing culture, culturing the oleaginous yeast in the membrane separation device, and subjecting the autotrophic microalgae to photosynthesis growth in the photobioreactor outside the membrane separation device, wherein the medium composition and culture condition are the same in the two reactors, and supplementing organic carbon source required by the growth and metabolism of the oleaginous yeast cells in the cultivation process. The photobioreactor also comprises a guide shell, the membrane separation device is arranged in the guide shell, and an outlet of the guide shell is leveling with the outlet of the photobioreactor; and the photobioreactor comprises a reflective membrane pasted on the outer surface of the guide shell. The method has the advantages of increasing the yield of autotrophic microalgae and oleaginous yeast cells, the utilization rate of an inorganic carbon source (CO2), and oil content of the microorganisms, and simplifying the culture device.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for cultivating oleaginous microorganisms, in particular to a method for semi-mixed culture of autotrophic microalgae and oleaginous yeast to obtain high yields of oleaginous microbial cells and simultaneously increase the oil content of oleaginous microbial cells . Background technique [0002] With the development of society and economy, the supply of fossil resources is becoming increasingly scarce, the contradiction between supply and demand of oil products is becoming more and more prominent, and the corresponding environmental pollution problems are also becoming more prominent. It is inevitable to develop renewable resources through multiple channels. Biodiesel derived from renewable resources such as microbial oil has high energy density, low sulfur content, sufficient combustion, and good lubricity. It is also renewable, environmentally friendly, easy to biodegrade, s...

AI Technical Summary

Problems solved by technology

However, since the mixed culture of yeast and algae is a heterotrophic culture, the level of oil accumulation in the cells is low; and the two kinds of cells harvest and extract oil together. Due to the different methods of oil extraction and the different requirements for breaking the wall, the effect of mixing them together is not good.

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