52 results about "Aegilops tauschii" patented technology

A kind of 3 specific amplimers and a method for labelling high-molecular glutelin subunit with it are disclosed. Based on the polymerase chain amplification reaction, the difference in DNA sequences of different high-molecular glutelin subunits and the high speed and efficiency of PCR are used to correctly detect if wheat or Jiejie wheat contains the high-molecular glutelin subunit Dxt 1.5 from the Jiejie wheat, or the high-molecular glutelin subunit Dx2 or Dx5 of wheat, or the high-molecular glutelin subunit Dxt2 or Dxt5 of Jiejie wheat. Its advantages are high speed, and high effect.

The invention relates to a cigarette nicotine reducing preparation formula, a preparation method of a cigarette nicotine reducing preparation and a blended tobacco shred preparation method. The formula comprises heat clearing and toxicity removing components such as cortex phellodendri, root of chinese barberry, hedyotis diffusa, honeysuckle stem, mesona chinensis, portulaca oleracea, schizonepeta and wikstroemia indica, addable lung moistening and phlegm removing components such as inula flower, cicada slough, momordica grosvenori, flos farfarae, sea clam shell, radix platycod and scaphium scaphigerum, heart-fire clearing and blood cooling components such as lophatherum gracile, salvia miltiorrhiza, jequirity, tabasheer, albizia odoratissima and aegilops tauschii, liver-fire clearing components such as mulberry leaves, polygonum cuspidatum, milk vetch, buddleja officinalis, seed of feather cockscomb and verbena officinalis, as well as stomach moistening and spleen tonifying components such as microcos paniculata, aristolochia kwangsiensis and ophiopogon japonicas, wherein all the components are 1-5 parts. The preparation method of the preparation includes the steps of medicinal material preparation, cleaning, drying, smashing, water addition and grinding, fermenting and the like. The tobacco shred preparation method includes tobacco smashing, water adding and grinding, fermenting, mixing with the preparation, filtering, pressing, drying, shredding, tobacco shred preparing and the like. Tobacco nicotine is lowered, the effect of expelling phlegm to stop cough is achieved, and diseases are prevented.

The invention relates to a novel method for obtaining artificially synthesized hexaploid wheat. The novel method comprises the steps of distant hybridization and obtaining the artificially synthesized hexaploid wheat. The distant hybridization includes that the distant hybridization is carried out with aegilops tauschii as a female parent and common wheat as a male parent, young embryo rescue is carried out through a hybrid embryo, and a test-tube plantlet is obtained; and the test-tube plantlet grows over a summer, manual doubling germination is carried out in November by means of colchicines, normal cultivation management proceeds, and aegilops tauschii-wheat octaploid seeds are obtained the next year. The steps of obtaining the artificially synthesized hexaploid wheat include that hybrid is carried out by means of the obtained aegilops tauschii-wheat octaploid seeds and tetraploid wheat with various good genes, and the hexaploid wheat is directly manually obtained. The novel method is easy to carry out, and the hexaploid wheat germplasm with a rich genetic background can be conveniently and manually synthesized.

The invention provides a tillage method for controlling aegilops tauschii as a malignant weed in wheat fields, which is implemented through the following steps: (1) in the first year before wheat seeds are sowed, deep ploughing is chosen by the tillage method, and the ploughing depth is 30cm; (2) in the second year before wheat seeds are sowed, deep ploughing is chosen by the tillage method, and the ploughing depth is 20cm; (3) in the third year before wheat seeds are sowed, shallow rotary tillage is chosen by the tillage method, and the ploughing depth is 10cm. The measure is adopted for two successive years, the seed bank of aegilops tauschii in soil can run out, and from the third year, the shallow rotary tillage mode which is commonly adopted by peasants at present can be adopted. The two-ploughing-and-one-rotary-tillage tillage method carries out ecological control and elimination, can effectively prevent and control the occurrence and harm of aegilops tauschii as a malignant weed in wheat fields, cannot destroy the environment and ecology, and has remarkable economic and social benefits for ensuring grain safety and food safety.

The invention belongs to the technical field of biotechnology and specifically discloses an aegilops tauschii antimicrobial peptide AtR100 and an application thereof. The inventor finally separates the aegilops tauschii antimicrobial peptide AtR100 with a sequence shown as SEQ ID NO.2, by establishing an aegilops tauschii cDNA library. The inventor finds that AtR100 has a strong inhibiting effectto the growth of tomato ulcer bacteria, ralstonia solanacearum, bacterial leaf streak of rice and clavibacter fangii which are difficult to be controlled by chemical drugs. A hemolytic test proves that the gene is harmless to mammals and humans. A powder acquired by performing liquid fermentation and spray drying on an antimicrobial peptide strain proves that the gene has a certain inhibiting effect to botrytis cinerea. The antimicrobial peptide strain is screened from aegilops tauschii. In later research, the antimicrobial peptide strain can be produced as a biological agent in large scale and can be applied to plant disease control.

The invention belongs to the technical field of pesticides, and discloses a wheat field and corn field weeding composition and a medicinal preparation thereof. The weeding composition comprises, by weight, 5-40 parts of oxadiazon, 2-30 parts of diflufenican and 2-20 parts of flufenacet. Different auxiliary materials are added to prepare missible oil, a suspoemulsion, a suspension, a wettable pulvis or a water dispersible granule. The weeding composition utilizes the different action mechanisms of the oxadiazon, diflufenican and flufenacet, and the above three herbicides are compounded to achieve synergistic effects. The above ternary herbicide composition can effectively control diseases of annual gramineous weeds and sedge weeds in the corn field and the wheat field, and especially has agood control effect on malignant weeds in the wheat field, such as bromegrass, Aegilops tauschii, wild oats and Lolium multiflorum; and test proves that the composition is safe to wheat, corn and next-stubble crops, and is an ideal wheat field and corn field herbicide composition.

The present invention relates to a transgenic plant which has integrated into its genome an exogenous polynucleotide encoding a polypeptide which confers resistance to Puccinia graminis f. sp. tritici, such as the Ug99 group of races Puccinia graminis f. sp. tritici. In an embodiment, the polynucleotide is the Sr33 gene from Aegilops tauschii.

The invention discloses a preparation method of null type HMW-GS (High Molecular Weight Glutenin Subunits) wheat. The method comprises the following steps: taking tetraploid wheat for expressing nullglutelin as a female parent, taking diploid aegilops tauschii for expressing non-null glutelin as a male parent, carrying out non-homoploidy hybridization to obtain triploid hybrid F1, and enabling chromosome to be automatically reduplicated into hexaploid; then taking the hexaploid as a female parent, taking Glu-D1 wheat for expressing the null glutelin as a male parent, carrying out homoploidy hybridization to generate F1 and selfing to obtain F2, planting seeds for identifying expression of the null glutelin in fields and selfing to obtain F3, selfing to obtain F6 through a single seed descent method of the seeds, and planting the seeds to obtain a plant line F7, thus obtaining a wheat line stably expressing 0 glutelin. Indexes including sedimentation value, the content of wet gluten, the gluten index, the forming time and the stabilizing time of the line are lower than those of weak-gluten wheat Chuannong 16, so that the line has the effect of reducing the related indexes of the wheat gluten.

The invention relates to the field of pesticides, and particularly relates to a complex herbicide for wheat after germination. The complex herbicide comprises a weeding effective component composed of traloxydim and mesosulfuron; in the weeding effective component, the weight ratio of traloxydim to mesosulfuron is (10-60) to (1-20). The herbicide disclosed by the invention is significant in effect; compared with single dosages of traloxydim and mesosulfuron, the preparation is relatively friendly to environment, is unlikely to be on fire in the transportation process, relatively safe, low in dosage, low in cost, safe to field crops, and obvious in fast-acting property; the control effect, the weed control effect on fresh weight and the comprehensive fresh weight control effect on monocotyledonous weeds and dicotyledon weeds in the field are improved; the weeds in the overall growth period of the crops can be prevented and killed by once pesticide application; and the complex herbicide is safe to next-stubble crops, especially has special effects on resistant weeds, namely aegilops tauschii and brome in North China, and is a relatively ideal herbicide for treatment of stems and leaves of wheat after germination at present.

The invention relates to the field of wheat genetics and molecular breeding thereof, in particular to development and application of a function mark of a powdery mildew resistant gene of aegilops tauschii. On the basis that the powdery mildew resistant gene M12147 of aegilops tauschii is subjected to map position clone, special structure sequences of candidate genes are used, a function mark SDAU-kasp2147 is developed, and verification is conducted by using 180 or more parts of aegilops tauschii natural germplasm and genetic offspring materials. The result proves that the function mark has theadvantage that the function mark is quick, direct and high in flux, is high in specificity, can complete screening and phenotype prediction work of the disease-resistant gene in mass materials in a short time, and can effectively serve for breeding work of wheat powdery mildew resistance.

The invention discloses quinoline amide compounds and a preparation method and application thereof. A structural formula of the quinoline amide compounds is shown as a formula I. According to biological activity verification, specific brassinolide reactions such as promotion of arabidopsis mutant det2-1 hypocotyl elongation in the dark and rice blade tilt angle increase, improvement of corn salt stress resistance and the like of some of the compounds are discovered; several compounds have high response values under the condition low concentration and are capable of inhibiting wheat plant height to retard growth and improve lodging resistance under the condition of high concentration; in addition, excellent effects in prevention and control of gramineous weeds such as wild oats, aegilops tauschii, barnyard grass, setaria viridis and aegilops. The quinoline amide compounds are easy to prepare, low in cost, high in agricultural application and promotion value and worthy of subsequent intensive research and development.

The invention discloses a post-seedling compound herbicide for wheat seedlings. The post-seedling compound herbicide comprises tralkoxydim and flucarbazone, the mass percent content of the tralkoxydim and the flucarbazone accounts for 0.1-85% of the total mass of the post-seedling compound herbicide, wherein the weight ratio of the tralkoxydim to the flucarbazone is (0.1-60) to (0.1-70). In comparison with a single medicament of the tralkoxydim and the flucarbazone, the use amount of the compound herbicide is low, the cost is low, and the prevention effect to monocotyledonous weeds and dicotyledon weeds in a field, the fresh weight prevention effect and the comprehensive fresh weight prevention effect are improved, and the security and the fast-acting property to the field crops are obvious. The weeds in the whole growth season of crops can be prevented and controlled through once pesticide application, the compound herbicide is safe to next-stubble crops, and especially has special effects to the resistance weeds such as aegilops tauschii and bromus japonicus in North China.

The invention provides a DNA bar code for discriminating aegilops tauschii and aegilops cylindrica and an application thereof. A nucleotide sequence of DNA bar code is shown in as SEQ ID NO: 1-2. By designing a pair of novel DNA bar code primers, an optimal identification sequence is obtained, the DNA bar code sequences of aegilops tauschii and aegilops cylindrica in a Genbank database are improved, aegilops tauschii, aegilops cylindrica and aegilops umbellulata can be distinguished by only one pair of the primers, and the unknown aegilops tauschii and aegilops cylindrica can be rapidly identified. Compared with a form characteristic identification method at a growth anaphase, the DNA bar code breaks up the restriction of an identification period, greatly increases the identification efficiency, increases the detection speed and accuracy, is in favor of guidance for timely controlling two wheat field worst weeds for an agriculture production mechanism and peasants, ensures the production safety of wheat and peasant income, reduces the threat to national food security, and has good economic benefit and social benefit.

The invention relates to the field of pesticides and herbicides, and discloses a triketone compound containing a quinazolinedione fragment, a preparation method and an application of the triketone compound, and a herbicide; the triketone compound has a structure represented by a formula (I). The triketone compound containing the quinazolinedione fragment and provided by the invention can be used as a broad-spectrum HPPD herbicide for preventing and killing gramineous weeds; in addition, the triketone compound containing the quinazolinedione fragment and provided by the invention has a good control effect on barnyard grass, green bristlegrass, crab grass, brome, wild oat, aegilops tauschii, alopecurus aequalis, alopecurus japonicus and other gramineous weeds which are difficult to control.

The invention discloses a method for widening tetraploid wheat genetic variation. The method comprises the following steps that parent tetraploid wheat AABB is utilized to hybridize with diploid aegilops tauschii DD, in-vitro culture is carried out on a hybrid young embryo ABD to obtain an ABD plant seedling, then chromosome doubling treatment is carried out to obtain low-generation artificially synthesized hexaploid wheat AABBDD, the low-generation artificially synthesized hexaploid wheat AABBDD is backcrossed with the same parent tetraploid wheat AABB to obtain a pentaploid wheat material AABBD, the pentaploid wheat material is used for selfing, chromosome behaviors such as chromosome exchange, translocation, inversion, deletion and insertion are generated through chromosome recombination in the selfing process, rich variation types are generated in genetic offspring, and a new tetraploid wheat material composed of different chromosomes is formed. According to the method, the chromosome of the tetraploid wheat can be induced to generate new structural variation in a relatively short time, and the method is high in beneficial mutation efficiency, rich in mutation type, environment-friendly, safe and low in cost.

The invention discloses herbicide synergistic composition for controlling aegilops tauschii in the wheat field. Effective constituents of the herbicide synergistic composition comprise pinoxaden, mesosulfuron-methyl, sesamin and mefenpyr-diethyl, and a weight ratio of pinoxaden, mesosulfuron-methyl, sesamin and mefenpyr-diethyl is 1-8:1:0.1-6:1-6. The herbicide synergistic composition further contains auxiliaries and solvents acceptable in pesticide. After addition of the auxiliaries and the solvents, the effective constituents including pinoxaden, mesosulfuron-methyl, sesamin and mefenpyr-diethyl in the herbicide synergistic composition account for 5%-80% of total weight of the composition. The dosage form of the herbicide synergistic composition can be a dispersable oil suspension, a dispersable oil suspoemulsion or emulsifiable concentrate. The herbicide synergistic composition is remarkable in synergism, and has a wide herbicide controlling spectrum and remarkable control effect onworst weeds such as the aegilops tauschii, lolium multiflorum and the like, thereby being weeding composition which is safe to wheat and synergistic for the wheat field.

The invention discloses pyrazole amide compounds as well as a preparation method application of the pyrazole amide compounds. The structural general formula of the pyrazole amide compounds is shown in the formula I (shown in the specification). Relevant biological activity tests find that certain compounds can generate specific brassinolide reaction and are capable of promoting the elongation of hypocotyledonary axis of an arabidopsis mutant det2-1 in the dark, increasing of dip angles of rice leaves, enhancing the salt stress resistance of corns and the like. An individual compound has a very high response value at a lower concentration, is capable of inhibiting the plant height of wheat, delaying the growth and improving the lodging resistance at a high concentration and simultaneously has very good effects of preventing and treating gramineous weeds such as wild oat grass, aegilops tauschii, barnyard grass, setaria viridis and goat grass. The series of the compounds are easily to prepare, low in cost, high in agricultural application and promotion values and worthy of being subsequently deeply researched and developed.

The present invention relates to a transgenic plant which has integrated into its genome an exogenous polynucleotide encoding a polypeptide which confers resistance to Puccinia graminis f sp. tritici, such as the Ug99 group of races Puccinia graminis f. sp. tritici. In an embodiment, the polynucleotide is the Sr33 gene from Aegilops tauschii.

The invention discloses picolinamide compounds as well as a preparation method and application of the picolinamide compounds. The structural general formula of the picolinamide compounds is shown in the specification. Relevant biological activity tests find that certain compounds can generate specific brassinolide reaction and are capable of promoting the elongation of hypocotyledonary axis of an arabidopsis mutant det2-1 in the dark, increasing of dip angles of rice leaves, enhancing the salt stress resistance of corns and the like. An individual compound has a very high response value at a lower concentration, is capable of inhibiting the plant height of wheat, delaying the growth and improving the lodging resistance at a high concentration and simultaneously has very good effects of preventing and treating gramineous weeds such as wild oat grass, aegilops tauschii, barnyard grass, setaria viridis and goat grass. The series of the compounds are easily to prepare, low in cost, high in agricultural application and promotion values and worthy of being subsequently deeply researched and developed.

The invention discloses an agricultural method for controlling Aegilops tauschii weeds in winter wheat fields. The method comprises seeding time adjustment, induction emergence, chemical seedling induction, emergence acceleration, weed killing with flames and rational close planting. The method has the benefits as follows: the method is safe, efficient, environment-friendly and convenient and quick to operate and only takes agricultural measures, no chemical herbicides are used, time and labor are saved, environmental pollution, ecological damage, crop pesticide residues and the like caused by use of chemical herbicides can be effectively reduced, so that green control on Aegilops tauschii in winter wheat fields in the production practice is guided, and the safety of agricultural products is improved.

The invention relates to a preparing method for a degradation agent for biological pesticide residue and belongs to the technical field of preparation of the degradation agents. The method comprises: firstly cultivating aegilops tauschii by using self-made cultivating soil, spraying pesticide and irradiating with X rays in a cultivating process, collecting aegilops tauschii straws on the surface of the cultivating soil, mixing the collected straws with water and then juicing, collecting a juiced solution and juiced residue, pouring the juiced solution into the cultivating soil containing aegilops tauschii roots for fermentation, mixing a fermented product with a glucose solution to obtain a fermentation mixture and filtering the fermentation mixture, collecting supernatant, carbonizing the juiced residue and then soaking a carrier in the glucose solution, and mixing the carrier taken out of the glucose solution with substances such as the supernatant and sodium alginate. The degradation agent for biological pesticide residue prepared through the preparing method provided by the invention is suitable for crops such as wheat, rice, soybean, corn, Chinese cabbage and leek, and is effective for pesticides such as fenpropathrin, dimehypo, carbofuran, carbofuran, dicofol, triazolone and methamidophos and is fast in degradation speed, and can be totally degraded in 9-10 days.

The invention belongs to the technical field of herbicide and provides a herbicide composition containing sulfentrazone and mesosulfuron. The weight ratio range of the sulfentrazone to the mesosulfuron is 80:1 to 1.3:1. After the sulfentrazone and the mesosulfuron are compounded, the weed control spectrums have complementary advantages, and good effect on flixweed, procumbent speedwell, bromegrassand aegilops tauschii is achieved. By the herbicide composition, the weed control spectrum is enlarged, the control effect is enhanced, rapid occurrence of herbicide resistance of the weed caused byusage of singe herbicide is avoided, occurrence of the herbicide resistance of the weed is delayed, the dosage is reduced, the use cost is reduced and environmental pollution is reduced.

The invention specifically relates to a winter wheat region wheat synthesizing method with high genetic diversity and a high embryo formation and seedling formation rate and solves the problems that existing synthesized wheat is not suitable for winter wheat regions, genetic information is limited, and the embryo formation and seedling formation rate is low. The winter wheat region wheat synthesizing method comprises the steps: a, choosing durum wheat and aegilops tauschii to be sowed; b, pollinating pollen of the aegilops tauschii to the durum wheat; c, performing cultivation and sterilization; d, inoculating young embryo of sterilized kernels to a culture medium II to be cultured; e, performing vernalization culture when seedlings are achieved; f, transplanting the seedlings to a land for growing field crops; g, performing soaking treatment through a colchicine solution with the concentration as 1% when the seedlings grow out tillers and then continuing performing field culture and management until the wheat is harvested. The synthesizing method disclosed by the invention is suitable for synthesizing and breeding a wheat novel material of the winter wheat region of northern China.

The invention relates to a method for identifying gibberellic disease resistance and particularly relates to the method for identifying gibberellic disease resistance of aegilops tauschii. The invention provides a method for high-throughput identification of the gibberellic disease resistance of aegilops tauschii by utilizing the aegilops tauschii in vitro fringe under controllable environmental condition within a short period of time. The method has the major advantage that the evaluation standard for the gibberellic disease resistance of aegilops tauschii is discovered and the gibberellic disease resistance of aegilops tauschii is evaluated according to the extension of the inoculated fringe. The standard is utilized to simply and quickly identify the gibberellic disease resistance of aegilops tauschii with 5-6 days. According to the method, the phenotype identification is direct and reliable, the repeatability is high, the resistance identification for a large batch of materials canbe completed within a short period of time and the method has significance in researching the gibberellic disease resistance of aegilops tauschii all over the world, finding a new gibberellic diseaseresistant resource and widening the gibberellic disease hereditary basis of the wheat.

The invention discloses an agricultural method for controlling Aegilops tauschii weeds in winter wheat fields. The method comprises seeding time adjustment, induction emergence, chemical seedling induction, emergence acceleration, weed killing with flames and rational close planting. The method has the benefits as follows: the method is safe, efficient, environment-friendly and convenient and quick to operate and only takes agricultural measures, no chemical herbicides are used, time and labor are saved, environmental pollution, ecological damage, crop pesticide residues and the like caused by use of chemical herbicides can be effectively reduced, so that green control on Aegilops tauschii in winter wheat fields in the production practice is guided, and the safety of agricultural products is improved.

The application of the invention belongs to the technical field of wheat molecular breeding, and particularly relates to serial molecular markers and an application thereof to pre-harvest sprouting gene molecular markers. The number of the molecular markers is 4, the molecular markers comprise an SSR marker and three KASP markers, wherein the SSR maker is an Xgwm383b marker, and the three KASP markers are A009711, A009180 and A009716. During variety breeding, the way of transferring excellent genes in aegilops tauschii to wheat is mainly to create a series of wheat / aegilops tauschii substitution line, and through distant hybridization means, aegilops tauschii chromosomes are introduced into wheat background. In accordance with the breeding method, practical F2 groups are used as an example, the serial molecular markers are designed to be used for detecting and tracking the situation of the aegilops tauschii chromosomes introduced into the wheat background, and further guiding pre-harvest sprouting resistant new species cultivation, and the serial molecular markers have better practical value.

The invention discloses an aegilops biuncialis specific molecular marker obtaining method and an application thereof. The method comprises the following steps: extracting genome DNA of aegilops biuncialis to be detected, and then simplifying genome library construction and high-throughput sequencing; comparing an SLAF tag obtained by sequencing after sequence analysis and screening of the SLAF tagof a specific gene with a reference genome sequence of known wheat; and screening sequences of which homology is less than 50% for primer designing; screening specific molecular markers of aegilops biuncialis, further positioning aegilops biuncialis primers obtained by screening to specific genes in aegilops biuncialis by utilizing aegilops tauschii genome donors with specific genes in homologousaegilops tauschii, and performing PCR amplification on the designed primers to obtain the specific molecular markers of aegilops biuncialis. The method can be used for detecting genetic components such as aegilops biuncialis chromosomes and chromosome fragments under a wheat background, can be used for rapidly detecting the wheat-aegilops biuncialis derivative line material on a large scale by simplifying a genome sequencing technology, and is time-saving, labor-saving and low in cost.

The invention discloses picolinamide compounds as well as a preparation method and application of the picolinamide compounds. The structural general formula of the picolinamide compounds is shown in the specification. Relevant biological activity tests find that certain compounds can generate specific brassinolide reaction and are capable of promoting the elongation of hypocotyledonary axis of an arabidopsis mutant det2-1 in the dark, increasing of dip angles of rice leaves, enhancing the salt stress resistance of corns and the like. An individual compound has a very high response value at a lower concentration, is capable of inhibiting the plant height of wheat, delaying the growth and improving the lodging resistance at a high concentration and simultaneously has very good effects of preventing and treating gramineous weeds such as wild oat grass, aegilops tauschii, barnyard grass, setaria viridis and goat grass. The series of the compounds are easily to prepare, low in cost, high in agricultural application and promotion values and worthy of being subsequently deeply researched and developed.

The present invention relates to use of Aegilops tauschii HMT (AetHMT1) gene, and belongs to the technical field of functional identification of genes. The present invention provides the use of AetHMT1 gene in improving plant stress resistance, total antioxidant capacity, selenium tolerance, and plant selenium absorptive and accumulating capacities. The AetHMT1 gene improves plant stress resistance, total antioxidant capacity, and selenium tolerance, and enhances plant selenium absorptive and accumulating capacities.