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Winter wheat region wheat synthesizing method with high genetic diversity and high embryo formation and seedling formation rate

A technology of genetic diversity and synthetic method is applied in the field of wheat synthesis, high genetic diversity and high embryo-forming seedling rate winter wheat area, which can solve the problem of limited genetic information, low embryo-forming seedling rate, problems such as inapplicability in winter wheat areas, to achieve the effect of broadening the genetic basis, simple operation process and wide selection range

Active Publication Date: 2018-12-18
CROP SCI RES INST SHANXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problems that existing synthetic wheat is not applicable to winter wheat regions, genetic information is limited, and the rate of embryo and seedling formation is low, the present invention provides a synthesis of wheat from winter wheat regions with high genetic diversity and high embryo and seedling rate. method

Method used

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  • Winter wheat region wheat synthesizing method with high genetic diversity and high embryo formation and seedling formation rate
  • Winter wheat region wheat synthesizing method with high genetic diversity and high embryo formation and seedling formation rate
  • Winter wheat region wheat synthesizing method with high genetic diversity and high embryo formation and seedling formation rate

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Embodiment 1

[0033] A method for synthesizing wheat with high genetic diversity and high embryo and seedling rate, using the following steps:

[0034] a. Durum wheat and goatgrass are selected for sowing according to the mass ratio of 2:1. Durum wheat is sowed in three phases, with an interval of 3 days in each phase, and goatgrass is seeded in the first phase;

[0035] b. Select durum wheat as the female parent, and Aegilops crassicarpa as the male parent, and emasculate and bag the female durum wheat. 48 hours after emasculation, pollinate and bag with newly collected Aegilops pollen;

[0036] c. 24 hours after pollination, cut the durum wheat plant 30 cm under the ear, rinse the cut part with tap water for 30 seconds, and then insert it into liquid medium I for cultivation. The cultivation temperature is 21°C, the cultivation humidity is 80%, and the light is 1500 lux. 8 hours of light per day, after 14 days of cultivation, the wheat grains are stripped from the ears for sterilization;

[0037]...

Embodiment 2

[0045] A method for synthesizing wheat with high genetic diversity and high embryo and seedling rate, using the following steps:

[0046] a. Durum wheat and goatgrass are selected for sowing according to the mass ratio of 2:1. Durum wheat is sowed in three phases, with an interval of 5 days in each phase, and goatgrass is sowed in the first phase;

[0047] b. Select durum wheat as the female parent, and Aegilops crassicarpa as the male parent, and emasculate and bag the female durum wheat. 72 hours after emasculation, pollinate and bag with newly collected Aegilops pollen;

[0048] c. After pollination for 24 hours, cut the durum wheat plant 40 cm under the ear, rinse the cut with tap water for 30 seconds, insert it into liquid medium I, culture at 25°C, culture humidity 80%, and light 2500 lux. 8 hours of light per day, after 14 days of cultivation, the wheat grains are stripped from the ears for sterilization;

[0049] d. Strip the sterilized immature embryos of wheat grains on a cl...

Embodiment 3

[0057] A method for synthesizing wheat with high genetic diversity and high embryo and seedling rate, using the following steps:

[0058] a. Durum wheat and goatgrass are selected for sowing according to the mass ratio of 2:1. Durum wheat is sowed in three phases, each interval is 4 days, and goatgrass is sowed in the first phase;

[0059] b. Select durum wheat as the female parent, and Aegilops crassicarpa as the male parent, and emasculate and bag the mother durum wheat. After emasculation, 51 hours after emasculation, pollinate and bag with newly collected Aegilops pollen;

[0060] c. 24 hours after pollination, cut the durum wheat plant 37 cm under the ear, rinse the cut with tap water for 30 seconds, insert it into liquid medium I, culture at 22°C, culture humidity 80%, and light 1900lux. 8 hours of light per day, after 14 days of cultivation, the wheat grains are stripped from the ears for sterilization;

[0061] d. Peel off the sterilized immature embryos of wheat grains on a c...

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Abstract

The invention specifically relates to a winter wheat region wheat synthesizing method with high genetic diversity and a high embryo formation and seedling formation rate and solves the problems that existing synthesized wheat is not suitable for winter wheat regions, genetic information is limited, and the embryo formation and seedling formation rate is low. The winter wheat region wheat synthesizing method comprises the steps: a, choosing durum wheat and aegilops tauschii to be sowed; b, pollinating pollen of the aegilops tauschii to the durum wheat; c, performing cultivation and sterilization; d, inoculating young embryo of sterilized kernels to a culture medium II to be cultured; e, performing vernalization culture when seedlings are achieved; f, transplanting the seedlings to a land for growing field crops; g, performing soaking treatment through a colchicine solution with the concentration as 1% when the seedlings grow out tillers and then continuing performing field culture and management until the wheat is harvested. The synthesizing method disclosed by the invention is suitable for synthesizing and breeding a wheat novel material of the winter wheat region of northern China.

Description

Technical field [0001] The present invention relates to the technical field of agricultural planting, in particular to a method for synthesizing wheat, in particular to a method for synthesizing winter wheat with high genetic diversity and high embryo and seedling rate. Background technique [0002] Common wheat (Triticum aestivumL, AABBDD, 2n=6x=42) is one of the main food crops in the world. Its sown area and output rank first in the world. It is the main food crop that accounts for 35% of the world's population. During the evolution of common wheat, only a very small number of cultivated two-grain wheat and goatgrass formed hexaploid wheat through natural hybridization, which caused the genetic diversity of hexaploid wheat to be greatly reduced compared with its parents, resulting in "evolution". bottleneck". [0003] Since the 1970s, due to the breeding and large-scale promotion of semi-dwarf high-yield wheat varieties, it has replaced the local wheat varieties with rich varia...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/001A01H4/008
Inventor 闫贵云左静静霍利光任永康左敏牛瑜琦
Owner CROP SCI RES INST SHANXI ACADEMY OF AGRI SCI
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