62results about How to "Broaden the genetic base" patented technology

The invention discloses a protein related to rice panicle type and an encoding gene and an application thereof. The protein is the a) or b) protein as follows: a) the protein consisting of an amino acid sequence shown as the sequence 4 in a sequence table; and b) the protein derived by substituting and / or deleting and / or adding one or a plurality of amino acid residues of the amino acid sequence shown as the sequence 4 in the sequence table and being related to the plant panicle type. Functional complementary experiments and transgenic experiments probe that the protein and the encoding gene thereof provided by the invention can control the plant panicle type, in particular to promote plants to show the erect panicle phenotype; and the protein and the encoding gene thereof enhance the research of the breeding of erect panicle type rice, thereby having important significance for expanding the genetic basis of the types of the existing erect panicle type and playing a significant role in genetic breeding of the rice.

The invention belongs to the field of cotton genetic breeding, and relates to a precocity short-fruit-spur upland cotton new-germplasm breeding method. The method includes the steps that hybridization and backcrossing are carried out with simian 2 as a female parent and gossypium klotzschianum as a male parent to obtain F1 plants and BC1F1 plants capable of normally growing; multi-generation backcrossing is carried out through simian 2 continuously, then backcross descendants are separated through selfing, zero type fruit spur families and first type fruit spur families good in prematurity and yielding ability are selected in separated groups, and precocity cotton new germplasm with the growth period of about 105 days, the zero-type or first-type fruit spurs, the compact plant type, concentrated boll opening, the good boll-setting performance, the high ginning outturn and the good fiber quality is bred through continuous multi-generation selfing and field orientation optimization in cooperation with an indoor plant laboratory test. The precocity source comes from the gossypium klotzschianum, and the hereditary basis of precocity breeding is expanded; precocity genes in the gossypium klotzschianum can be used by breeding and are of great significance in science and production.

The invention provides a brassica napus cytoplasmic male sterile restore line population improvement method. The method comprises the following steps: a restore line is taken as a female parent and is hybridized with a plurality of inbred lines, a first filial generation selects a fertile plant as a female parent for backcross and inbred for a generation, restore gene locus homozygous single plants are selected through identification, equivalent seeds are taken from each single plant and mixed to form an S0 population, the S0 population is sown, a chemical inducer is sprayed to the female parent, open pollination is carried out in the population to harvest chemically-induced sterile plant seeds to form a recurrent selection population S1, then recurrent selection is carried out for at least 6 times, and a DH single plant obtained by carrying out field planting and microspore culture on a screened target single plant is a brassica napus cytoplasmic male sterile restore line. According to the method, a chemical induction method is initiatively adopted to acquire a sterile plant, so that the time and the labor are saved, and finally, the genetic basis of the restore line is wide; and more importantly, in the process of applying a chemically-induced sterile plant to carry out recurrent selection, the ratio of restore genes in the recurrent population is stable to be 100 percent.

The invention relates to a method for obtaining hybrid seeds of water lily varieties and belongs to the technical field of plant breeding. The method comprises the main technical measures of after intervarietal hybridization is performed, performing asepsis treatment on pollinated 14-16-days ovaries after pollination, taking out embryos from the ovaries, and cultivating the embryos serving as explants in a culture medium with 1 / 2MS added with 1.5mg / L of 2,4-D of and 2.0mg / L of 6-BA, performing subculture and expanding propagation to obtain seedlings, and transferring the seedlings into a culture medium with MS added with 0.2mg / L of NAA for rooting, wherein the culture temperature is 20-25 DEG C, illumination time is 10-12 hours every day, and the illuminance is 1,600-2,000lx. According to the method, the key technical problem of obstacle caused by hybridization and fertilization of the water lily varieties can be solved, and seedlings of the hybrid seeds of the water lily varieties can be obtained.

The invention discloses a method for breeding a normal corn inbred line by using a hard grain type waxy corn inbred line. According to the method, a corn inbred line is bred with a method of one-time hybridization of the early-maturing hard grain type waxy corn inbred line and repeated backcrossing of normal corn, so that rapid breeding of a new normal corn species with excellent quality, high unit weight and early maturity is realized. With the adoption of the method, the quality of the normal corn inbred line is improved, the hereditary basis of normal corn is widened, types of the normal corn are enriched, the corn growth period is shortened, and the method has the important social significance and economic value.

The invention relates to a method for breeding homotype waxy corn selfing line by sweet corn selfing line. The method comprises the steps of (1) selecting waxy corn resource as female parent and sweet corn selfing line as male parent to obtain cross-bred clusters; (2) planting seeds of the cross-bred clusters, and taking sweet corn selfing line as male parent to obtain backcross clusters; (3) threshing the backcross clusters, selecting and remaining non-sweet seeds, planting single seeds, carrying out single plant bagging selfing, selecting clusters with waxy seeds, threshing single clusters, and selecting and remaining waxy non-sweet seeds; (4) planting the waxy non-sweet seeds selected and remained by step (3), repeating three cycles from step (1) to step (3) to obtain the waxy corn selfing material which has the same type as the sweet corn selfing line; and (5) carrying out single seed planting on the selfing material obtained in step (4), carrying out single plate bagging selfing, and selecting the clusters without sweet seeds to be threshed to obtain the waxy corn selfing line which has the same type as that of the sweet corn selfing line.

The invention relates to the field of soybean molecular breeding, in particular to a QTL related to soybean hundred-grain weight, a molecular marker, an amplification primer and application. Cultivated soybeans and wild beans are used as parents to construct a genetic population, the genetic population is positioned to one QTL related to the soybean hundred-grain weight, the QTL is located in the 1641719bp-1795446bp interval of the No.7 chromosome of the soybeans, the marker interval is only 0.15 Mb, and a molecular marker SSR-09-089 related to the hundred-grain weight is identified and can be used for molecular marker-assisted selective breeding and mining of related functional genes; meanwhile, wild resources can be utilized, and the genetic basis of soybean cultivation is widened.

The invention provides a method for breeding an improved mutton sheep strain, which includes the steps: firstly, using a plurality of rams as strain ancestors to mate with 30-50 ewes respectively so as to establish a plurality of families; secondly, performing beneficial-trait within-family selection to more than four generations of the families established in the step 1; thirdly, subjecting the families selected in the step 2 to family hybridization; fourthly, using hybrids obtained in the step 3 and family non-hybrids to establish a strain breeding core group, and grouping other individuals as a production group; fifthly, subjecting the core group established in the step 4 to beneficial trait selection, opening the core group, allowing selected qualified individuals in the production group to enter the core group, introducing 'foreign blood' timely to avoid inbreeding depression; and sixthly, obtaining the mutton sheep strain with beneficial traits adaptable to local environment by multi-generational breeding. By using the method to breed the improved mutton sheep strain, dependence on foreign varieties is lessened, and the current situation of 'all introduction and propagation and no breeding' in the field of breeding sheep introduction in China is changed.

The invention discloses a stem-segment <60>Co-gamma ray radiation mutation breeding method for sweet potato. The method provided by the invention comprises the steps of seedling collection, mutation treatment, transplanting and management after transplanting, and continuous cutting propagation and artificial selection of mutant individuals. According to the invention, a treated material is a stemsegment (comprising terminal buds and a great amount of lateral buds), so large quantities of materials can be treated by one time, and the breeding period is effectively shortened; through a vegetative propagation manner of continuous cutting, generation of a chimera is overcome, and stable and uniform variant plants are obtained; meanwhile, by adoption of <60>Co-gamma ray radiation for mutationof the stem segment of the sweet potato, rich genetic variation can be created; the genetic basis of the sweet potato is broadened; a simple, convenient and highly-efficient method is provided for breeding of new varieties of the sweet potato; and the genetic basis of germplasm resources of the sweet potato is broadened.

The invention relates to a method for obtaining hybrid seeds of water lily varieties and belongs to the technical field of plant breeding. The method comprises the main technical measures of after intervarietal hybridization is performed, performing asepsis treatment on pollinated 14-16-days ovaries after pollination, taking out embryos from the ovaries, and cultivating the embryos serving as explants in a culture medium with 1 / 2MS added with 1.5mg / L of 2,4-D of and 2.0mg / L of 6-BA, performing subculture and expanding propagation to obtain seedlings, and transferring the seedlings into a culture medium with MS added with 0.2mg / L of NAA for rooting, wherein the culture temperature is 20-25 DEG C, illumination time is 10-12 hours every day, and the illuminance is 1,600-2,000lx. According to the method, the key technical problem of obstacle caused by hybridization and fertilization of the water lily varieties can be solved, and seedlings of the hybrid seeds of the water lily varieties can be obtained.

The invention discloses a germplasm improvement and breeding technology system of step-by-step classification of heterozygous groups of fresh-eating corn, including selection of excellent germplasm and hybrids of the initial population, selection of resistance, double haploid breeding, DH line testing, combining ability determination, DH line is used in breeding and assembling the second cycle of the original population and other steps. The present invention advocates the reincarnation selection of multi-pest resistance, lodging resistance, quality, yield, etc. under multi-ecological conditions, and performs group exchange and mutual combination to aggregate good genes such as high-quality, high-yield, multi-resistance, and wide-adaptation. , so that the excellent characteristics of the population are gradually accumulated, and the breeding performance is gradually improved.

A method for preparing the distant hybrid of chrysanthemum by embryo rescue includes hybridization between wild diplontic chrysanthemum and cultured hexaplontic 'Huang Ying', embryo rescue by ovary culture, discriminating their descendants by different approaches, and choosing the descendant with new characters.

The invention discloses a protein related to rice panicle type and an encoding gene and an application thereof. The protein is the a) or b) protein as follows: a) the protein consisting of an amino acid sequence shown as the sequence 4 in a sequence table; and b) the protein derived by substituting and / or deleting and / or adding one or a plurality of amino acid residues of the amino acid sequence shown as the sequence 4 in the sequence table and being related to the plant panicle type. Functional complementary experiments and transgenic experiments probe that the protein and the encoding gene thereof provided by the invention can control the plant panicle type, in particular to promote plants to show the erect panicle phenotype; and the protein and the encoding gene thereof enhance the research of the breeding of erect panicle type rice, thereby having important significance for expanding the genetic basis of the types of the existing erect panicle type and playing a significant role in genetic breeding of the rice.

The invention discloses a culture medium for in-vitro directional screening of a peanut high oil body and a screening method, and belongs to the technical field of peanut breeding. The culture mediumfor in-vitro directional screening of the peanut high oil body comprises a somatic embryo germination and screening culture medium, a seedling formation and screening culture medium and a recovery culture medium; the somatic embryo germination and screening culture medium takes an MS culture medium as a basic culture medium, and 3-5mmol / L HYP and 3-5mg / L BAP are added; the seedling formation and screening culture medium takes the MS culture medium as a basic culture medium, and 7-9mmol / L HYP and 1-3mg / L BAP are added; and the recovery culture medium takes the MS culture medium as a basic culture medium, and 1-3mg / L BAP is added. The culture medium is adopted to screen the peanut high oil body, the screening efficiency can be improved, and the seedling hardening time of screened plants in the later period can be effectively shortened.