Method for obtaining chrysanthemum distance hydrid using goung embryo rescue
A technology for the rescue of young embryos and distant hybrids, which is applied in the fields of botanical equipment and methods, applications, and plant genetic improvement, can solve problems such as difficult gene introduction into cultivated chrysanthemums, and achieve the effect of broadening the genetic basis.
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[0031] The method for the rescue of immature embryos of distantly related hybrids of the genus Chrysanthemum and the identification of hybrids provided by the present invention is implemented as follows:
[0032] 1. Obtaining the progeny material of Chrysanthemum interspecific hybridization
[0033] 1.1 Material selection
[0034] Select the diploid wild species Chrysanthemum (D. ". Nanjing: Jiangsu Science and Technology Press, 1993, 4).
[0035] 1.2 Rescue of immature embryos and hybridization
[0036]Hybridization was carried out in the greenhouse. The diploid wild chrysanthemum brain was used as the female parent to cross with the cultivated chrysanthemum 'Huangying'. The female parent was artificially detasseled and bagged for isolation. The male parent was bagged when the tongue-like flowers were slightly open. Pollination is performed at 9-10 o'clock or 3-4 o'clock in the afternoon, and the same inflorescence is pollinated twice. After hybridization, the method of o...
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