The invention belongs to the field of seed breeding in
biotechnology, and relates to a method for cultivating
cucumis sativus L
introgression line material for resisting
meloidogyne incognita. The method comprises the following steps of: performing the
interspecific hybridization of
wild species and cultivated species, doubling chromosomes to obtain new amphidiploid species, and performing backcross and
selfing to obtain an interspecific
introgression line; identifying the
cucumis sativus L
introgression line by a method of inoculating artificial
seedling stage spawn liquid to obtain the
cucumis sativus L
interspecific hybridization introgression line material for resisting
meloidogyne incognita, wherein an identification result indicates that
disease indexes of the introgression line 10299F-5 are 2.0, and the introgression line 10299F-5 has
disease-resistant expression. The cultivating cucumis sativus L
interspecific hybridization introgression line material for resisting meloidogyneincognita is high in an economical character, and can be used as a breeding material directly, so the blank of cucumis sativus L resources for resisting
meloidogyne incognita is filled, a reference is provided for the
inoculation identification of other plants, and useful
genetic materials are provided for improvement on cucumis sativus L species, the transfer of
disease-
resistant genes,
gene positioning, molecular marks,
gene cloning and the like in future.