The invention discloses a cherry
rootstock tissue culture medium, which is prepared from 2 to 60 ml / L of optimized MS base mother solution, 2545 g / L of
sucrose, 6.5 to 7.0 g / L of
agar, 0.4 to 0.6 mg / L of 6-Benzylaminopurine, 0.1 to 0.3 mg / L of
indolebutyric acid, 0.061 to 0.067 mg / L of VC, 0.05 to1.00 mg / L of indoleacetic acid and 1000 to1200 mg / L of
active carbon; the culture medium is specifically applied to the growth and cultivation of the
bud of the dwarf cherry
rootstock, the cultivation of the strong stock and the cultivation of the induced root; the proliferation and root-growing effect is excellent so that the root-growing rate of the dwarf cherry
rootstock can reach 95% to 98%; the root is rough and tidy; the culture
bud is healthy; the leaf is green, therefore, the labor and material are effectively saved; the
survival rate of the transplant can reach 95%, and the foundation is established for the large-scale production. The
tissue culture medium method disclosed by the invention is simple and easy, thereby simplifying the program, improving the efficiency, and reducing the cost. In the invention, the problems of brown
stain and
vitrification in the culture process of
tissue culture medium can be solved; and therefore, the invention can be applied to the fast
reproduction of the Gisela sweet dwarf cherry rootstock in the large-scale production.