162 results about "Dwarfing" patented technology

The invention provides a dwarfing, close planting and early fruiting culturing method for lychees with the shape of a chicken beak. The culturing method comprises the following steps: grafting, namely selecting Sapindaceae dwarfing rootstocks and adopting scion grafting method to perform cleft grafting; field setting, namely arranging the planting holes in rectangle or staggered different line manner; nutrient and water management, namely primarily adopting the method of frequent and low-amount fertilization, applying phytocide, and utilizing a mulching film; shaping and trimming, namely controlling each tree crown to be an expanding heart shape with two layers and seven leader branches through trunk fixing, bud carving and branch trimming, and controlling tree height through cutting the trunk in a ringing manner, trimming in a retractable manner in the winter, and promoting growth of new buds through cutting main branches and side branches in a ringing manner; drug spraying, namely applying growth regulation substances; insect disease prevention. The culturing method adopts the dwarfing rootstocks which is in the same family as the lychees with the shape of the chicken beak to complete grafting; the tree height and tree crown shape are controlled through trimming; the seedlings of lychees with the shape of the chicken beak is shortened through applying drugs; the fertility is reserved through utilizing the mulching film; the sunlight utilization ratio is increased, and early fruiting and high yield of the lychees with the shape of the chicken beak are obtained; the culturing method provided by the invention is quick in bringing production, early in fruiting, high in yield, and good in fruit quality.

The invention relates to a mature mango breed rapid updating dwarfing culture method. Cutting trunk time has no season restriction, grafting is immediately carried out after cutting trunk, cut grafting method is adopted, plastic film is adopted to cover grafting sprout, and tearing film is used for banding, and management of plant diseases and insect pests and intertillage fertilization are cared after grafting. The invention has the beneficial effects that: when the method is used for rapidly replacing breed, dwarfing and especially improvement of breed of mature mango garden and inferior mango garden, on one hand grafting survival rate is improved, breed improvement period is shortened, on the other hand, the improved fruit garden goes into operation early, economic benefit is increased, the aim of dwarfing the crown of mango tree can be realized by adopting the method, fruit picking and plant diseases and insect pests operation are easier, labour and time are saved, and management cost is reduced.

The invention provides a method of developing a corn dwarfing material via gene editing. The method comprises designing, for target gene ZmGA20ox3 / ZmGA20ox5 of corn, a sgRNA based on CRISPR / Cas9, connecting a fragment having sgRNA-coding DNA to a Cas-carrying carrier, and converting the corn with the constructed carrier to achieve site-directed mutagenesis of the gene ZmGA20ox3 / ZmGA20ox5 so as toobtain a transgenic corn plant with corresponding gene function deficiency. The biological functionality of the corn gene ZmGA20ox3 / ZmGA20ox5 is disclosed herein for the first time; the corn gene ZmGA20ox3 / ZmGA20ox5 is edited through CRISPR / Cas9 technology; a mutant material with no transgenic gene insert fragment is acquired through further screening; the corn dwarfing material has important breeding value.

The invention relates to fruit tree grow seedling fields, more specifically it discloses a nursery method of dwarfing pear trees which includes raising rootstock, dwarfing interstock and scion. The main technology is said rootstock use the seedling of wild-anvil pear seeds as the root grafting, said dwarfing interstock is hybrid, that is manual crossing between the wild pears as female parent line and the Huagai pears and the Nanguopears which are paternal, the hybrid sapling raising from the hybrid seeds is used as the dwarfing interstock, said scion is the dwarfing interstock is grafted on the rootstock (seedling) as the scion, then the dwarfing pear trees are raised. The affinity between the rootstock and the graft is strong in the invention, tree bodies' compact, fruiting early, crop high, cold resistant, disease resistant, easy management, convenient management and it is suitable for landscaping, greening and potted plants in rural and urban.

The invention discloses a pansy-factory-like plug seedling method, which comprises the following steps: (1) preparing matrix and filling plug; (2) sowing; (3) accelerating germination; (4) seedbed management; and (5) pansy dwarfing and hardening-seedling. The method has the advantages of creating four core technologies of standardization management of pansy-factory-like plug seedling, setting the quality standard of pansy-factory-like plug seedling products, increasing the seedling production efficiency, reducing the cost, being beneficial of unified management and promoting new technologies and the like, and therefore manufacturers are directly benefited.

The invention relates to a dwarfing and pest-killing corn plant core-removing agent, which comprises the following raw material medicaments in percentage by weight: diethyl aminoethyl hexanoate 0.05-2.5, ethephon or paclobutrazol 0.1-3, chlorpyrifos 0.3-3, imidacloprid 0.01-0.09, pirimor 0.1-2.5 and auxiliary the balance. The medicament can complete pest prevention and control and growth regulation by one time of core-removing medicament application, wherein in one time of core-removing medicament application, various corn pests such as ostrinia nubilalis and aphid are prevented and controlled; the average corn plant height is reduced by 20 to 50 centimeters; the prevention and control rate of the ostrinia nubilalis and core-eating worms is over 80 percent; the prevention and control rateof the aphid is over 95 percent; and the corn yield is increased by more than 20 to 50 percent. The medicament is simple convenient in application, labor-saving, medicament-saving and safe, prevents harm caused by the ostrinia nubilalis and aphid in a whole period of growth of corns and completely solves the problem of pest prevention and control in a post-period of corns, thereby increasing cornyield and harvest, reducing environmental pollution and being worthy to be promoted and used.

The invention discloses a protein relevant to plant height, a coding gene and an application thereof. The protein provided by the invention is the protein as the following (a) or (b): (a) a protein comprising an amino acid sequence shown in a sequence 1 in a sequence table; (b) a protein which is formed by substituting and / or deleting and / or adding the amino acid sequence of the sequence 1 by one or plural amino acid residues, is relevant to the plant height and is derived from the sequence 1. The relevant protein for regulating and controlling the development of the plant height mainly controls the plant height, can cause the dwarfing of plants after being expressed, is dominant relatively to normal high plants and is an important dwarfness source when being applied to the work such as plant hybridization, seed breeding, utilization and the like. A dominant short stalk gene is adopted as the dwarfness source, which is easy to solve the problem of plant height, has more extensive sieving range of parents and is beneficial to distant hybridization comprising the selective matching of the hybridized parents between indica rice and japonica rice and between subspecies.

The peony dwarfing pot culture method includes planting peony plant in flower pot, applying nutrient agent as base fertilizer or dressing, irrigating with dwarfing agent and water to constitute peony root system environment with the pot culture medium, nutrient agent and dwarfing agent with optimal liquid, gas and solid proportion for peony to grow and automatically balanced hydrogen ion density. Pot culture for 6 years can obtain peony plant with short height than that in field and the same growth period and physiological process as that in field. The present invention makes it possible for potted peony to enter household.

The invention discloses a method for trimming and pruning grapes in a greenhouse. The method for trimming and pruning grapes in the greenhouse comprises the steps of cultivation of a 'single trunk and double arms' tree form and low-position trimming, wherein the cultivation of the 'single trunk and double arms' tree form needs to be finished during the first year of planting so that the tree form can be fixed and low-position trimming is carried out yearly during winter trimming after cultivation so that a fruit part can be fixed. According to the method for trimming and pruning grapes in the greenhouse, a grapevine in the greenhouse can be trim, the length of the 'single trunk' is kept 0.7-0.8m, a dwarf grapevine is obtained, the 'double arms' are stabilized beside an iron wire, the length of each arm is 0.5-0.6m, epicormic branches on the double arms are kept 1.5-1.8m during a year, the photosynthesis can be carried out fully due to the formed 'single trunk and double arms' tree form, and operation and management are facilitated due to the fact that the fruit part is stabilized beside the iron wire through the low-position trimming. Therefore, the method for trimming and pruning grapes in the greenhouse has the advantages that technical guidance is provided for achieving dwarfing of the grapevine, stabilizing the fruit part, maintaining the reasonable tree from, and improving the economical life of the grapes, and great ecological benefits and economic benefits can be achieved.

The invention discloses a planting method for grapes with high yield. The planting method comprises the following steps: loosening soil and erecting scaffolding; applying base fertilizer; planting; carrying out topdressing; pruning; preventing plant diseases and insect pests; picking up. According to the planting method disclosed by the invention, selection of planting raw materials and a technical method are safe and non-pollution; at the same time, the planting method is in line with a growth rule of the grapes; on the one hand, the requirements of grape growth on various nutrients can be met; on the other hand, waste of resources and land destruction brought by excessive use of fertilizer can be avoided, thereby achieving greenness and environmental protection; in addition, dwarfing treatment is carried out on the grapes so as to enable the heights of trunks of grape vines to be less than 1.6 meters, and the dwarfing treatment is started from seedlings, so that the effect is obvious; the dwarfing treatment is beneficial to improve the yield of the grapes; meanwhile, the growth of the grapes is accelerated, and the aims of increasing the yield and incomes are achieved; after the grapes are ripened, the grapes have the advantages of low pesticide residue, rich nutrition in fruits, bright luster, delicious taste, good mouth feel, better color and more sufficient sugar content.

The present invention is one kind of turf grass dwarfing and water saving agent compounded with uniconazole 5-7 weight portions, triapenthenol 7-14 weight portions, paclobut4razol 9-12 weight portions, daminozide 1.8-7.2 weight portions, chlormequat chloride 1.3-5.2 weight portions, mepiquat chloride 1.3-5.2 weight portions, flurprimidol 1-4.5 weight portions, sticker 0.1-1 weight portions, and water assistant 0.1-1 weight portions. The present invention has the effects of dwarfing turf grass, saving water, etc.

The invention discloses a method for cultivating North America holly dwarf products through upper air layering. The method includes the following steps that in the growth and development period of North America holly fruits, a branch which is 20-60 cm in length and about 0.3-1.5 cm in diameter, grows vigorously, is free of plant diseases and pests, and has dense fruits is selected, the 5-10 cm position of the lower portion of the branch is girdled, the girdling width is controlled to range from 0.5 cm to 1.5 cm, a cambium is completely removed, the girdled position is coated with a root inducing agent mixed solution, then the girdled position is coated with a layering medium with the water content ranging from 60% to 80%, finally, the girdled position is wrapped by a plastic film, then an opening and a lower opening are tied, and wetness is kept. 60-70 days later, the medium is full of root systems, the rooting branch is cut off from the wrapping position of the medium, part of leaves are removed, then the rooting branch is transplanted in a culture medium with the ratio of peat to perlite of 3:1(V:V), the medium and the environment are kept wet, the branch is put in shade for seedling recovering for about 20 d, and the survival rate is 96.2% or above. The method has the advantages of being easy and convenient to operate, high in maneuverability, low in cost and high in survival rate, and is an important technical means for achieving dwarfing and miniaturization of North America holly products.

The invention discloses a papaya dwarfing planting method, which comprises the following key steps of: root deepening, ridging, twisting, water control and biological control. Through the physical mandatory twisting technology, a trunk is shortened, the wind resistance is improved, the ordinary production management is facilitated, and the production period is prolonged; through twice or more twisting, the posture of the trunk is adjusted, and the center of gravity of the trunk is regressed; in the process of twisting, partial tissues and nutrient transporting pipelines in a stem rod are mechanically damaged, the trunk can be prevented from spindling, the accumulation of nutrients at the bottom is reinforced so as to crosswise thicken and enlarge the stem rod of a papaya fruit tree, and the pitch between leaves is obviously shortened; and through the application of biological active substances and microbial agents, the growth environment of a papaya root system can be improved, the robust growth of the root system is promoted, and the purposes of dwarfing, compact planting, rod strengthening and high yield are effectively achieved.

The invention discloses a growth retardant treatment based potted drumstick tree dwarfing and fertilizing method. The method comprises the following steps of: selecting full and non-moth-eaten drumstick tree seeds, putting the drumstick tree seeds in an illumination incubator to undergo primary culture, according to different growth periods of drumstick trees, taking peat soil, perlite and vermiculite as matrices, applying a nutrient solution and reasonably controlling water and carrying out composite foliage spray and root application by using growth retardants including paclobutrazol and chlormequat chloride, wherein the nutrient solution contains potassium nitrate, ammonium nitrate, magnesium sulfate heptahydrate, monopotassium phosphate and calcium chloride dehydrate. The problems that the branches and trunk of the potted drumstick trees are single and too high and are easy to fall, the branches and leaves are scarce and are easy to fall off, the leaves are small and are easy to fall and the leaves are a bit yellow are effectively solved by adopting the growth retardants and the fertilizing method.

The jatropha yield increasing and stabilizing cultivation process includes the following steps: growing seedling; transplanting; pruning and shaping through topping-off and shaping to form turned umbrella tree after the jatropha tree grows to 1-1.5 m height and maintaining the tree height during blossom of 2-2.5 m and fruit bearing all the year round; and applying balancing nitrogen fertilizer, phosphate fertilizer and potash fertilizer. The cultivation process has fast growth, increased and stabilized yield, low consumption and easy picking of jatropha fruit.

The invention discloses a scarf joint method of Canarium album ReauschsRaeusch. The method sequentially comprises the following steps of: selecting stock trees, selecting scions, carrying out scarf joint, fixing, managing after scarf joint, and managing the grafted trees which are subjected with to scarf joint for a long term. The scarf joint method of the Canarium album Reaaeuschs has the following advantages of: 1, improving the survival rate, wherein the survival rate of the grafted trees is improved to above 90% from the current 30-50%; 2, realizing dwarfing, wherein the height of the fruit-bearing trees are is descended to below 5 meters from 10 meters; 3, realizing close planting, wherein the plantings at every mu are increased to 30 plants from 12 plants; 4, realizing early bearing, wherein the trial period after planting is decreased to 4 years from 7 years, and the startup time is decreased to 6 years from 10 years; 5, realizing high yield, wherein the yield is improved to 1500 kilograms, but the traditional planting yield per mu is 600 kilograms; and 6, realizing high quality, wherein, comparing with seedling cultivation, a fine variety generation is cultivated through scarf joint with unseparated properties, and fine variety performance can be kept.

The invention discloses SNP markers closely linked with a height trait of cabbage type rape, and application of the SNP markers. By using an EMS induced mutation technology, a dwarf mutant DF09 with the plant height being 65 cm is obtained. The dwarf mutant DF09 is taken as a research material, a method of map-based cloning is adopted, and a dwarf trait control locus BnDwf.C9 is subjected to finemapping in the 132 Kb interval of a C09 chromosome. In the fine mapping interval, three SNP molecule markers of BnaC09-42, BnaC09-46 and BnaC09-54 which are closely linked with a dwarf trait are obtained. According to the SNP markers closely linked with the height trait of the cabbage type rape, and application of the SNP markers, through a five primer amplification blocked mutation system technology (the BnaC09-42, the BnaC09-46 and the BnaC09-54) or a conventional PCR amplification technology (BnaC09-46pcr), a dwarf material can be accurately screened according to gene types, thus the dwarfmaterial is applied to molecular identification of early target traits, the progress of dwarf breeding is accelerated, and a foundation is laid for clone of dwarf genes.

The invention relates to a method for cultivation and colonization of rooty dwarf vallisneria natans. The method includes preparing rooty dwarfing nutrient solution, preparing cultivation basic substances, making a colonization device, immersing vallisneria natans in the rooty dwarfing nutrient solution, and transplanting the vallisneria natans into the colonization device. The colonization device is formed by an upper-layer hemp rope net, a lower-layer hemp rope net and the cultivation basic substances. Rooty and short plants are grown in the nutrient solution, deposit releasing nutrition salt can be suppressed during water ecology restoration, and meadow type underwater landscape is formed. The cultivation and colonization device can be used to cultivate seedlings and satisfies construction needs in a room for all year round, and the colonization device covers the deposits and suppresses the nutrition salts from being released. Materials used are environmental friendly and nontoxic. The speed and quality of water ecology restoration are improved.

The invention discloses a macadamia nut dwarfing method. The grafted scion woods are sprayed and moisturized with low-temperature sterile water before the grafted scion woods are irradiated by the gamma rays, the grafted scion woods can be prevented from withering, and the grafting survival rate is increased; the illumination intensity of the macadamia nut grafted seedling in the daytime is properly weakened, and the light of the macadamia nut grafted seedling is increased at night, so that the rhizomes of the macadamia nut grafted seedlings are thickened, and meanwhile, the transverse growthrate of the tree bodies is accelerated; konjac glucomannan can be added into the root treating agent, so that the soil penetration depth of the macadamia tree root system can be increased, sufficientnutrients are improved for macadamia nut trees, and the premature senility of the macadamia nut trees can be effectively prevented.

The invention discloses a cedrela sinensis trunk obliquely-pulled dwarfing and compact planting cultivation method, and belongs to the field of plant cultivation. According to the method, red oil cedrela sinensis seedlings or black oil cedrela sinensis seedlings which are excellent in seed source, 1-2 meters in seedling height, 1-2 years in seedling age, strong in growth and free of plant diseases and insect pests are selected, and the seedlings are planted in a double-line large-line-spacing and small-row-spacing cultivation mode. After 2-3 times of sprout picking in the current year, obliquely pulling is carried out on trunks in a binding method so as to promote the seedlings to grow more lateral branches; the seedlings are cultivated in multiple-main-branch dwarfing tree shapes and trees are controlled at a reasonable height by combining the pruning technology. According to the method, nutrient consumption of tree bodies can be reduced, the yield of cedrela sinensis sprouts can be improved, and the cultivation period of dwarfing and compact planting of tree shapes can be shortened; meanwhile, the picking difficulty of the cedrela sinensis can be effectively reduced, work efficiency is improved, and probabilities of plant diseases and insect pests on the tree bodies can be reduced to a certain extent.

The invention discloses a seedling culture matrix and a preparation method thereof and relates to the field of plant seedling culture. The seedling culture matrix is prepared from, by mass, sandy soil, magnesium sulfate, thoroughly decomposed cow dung, a water absorption water-retaining agent, carbonized rice husks, palm bark fibers, calcium magnesium phosphate fertilizer, nitrogen-phosphorus-potassium mixed fertilizer, ammonium sulfate, monoammonium phosphate, boric acid, polyaspartic acid, glucosamine oligosaccharides, a plant disease protective agent and shock-resistant bacteria. By the adoption of the technical scheme, the matrix has the functions that fertilizer efficiency is sufficient, nutrients are slowly released, disease resistance and stress tolerance are achieved, the nutrients are comprehensive, and pertinence is high, and all indexes reach industrial standards. Through volume production and use in multiple seedling culture factories, the seed germination rate and emergence rate can be increased, seedlings emerge in order and grow vigorously, the seedlings are strong and are free of excessive growth, root macerating, dwarfing and ossification, flower bud differentiation of the seedlings is normal, and the properties, yield and quality of crop varieties are guaranteed.

An agent for improving lawn grass quality, which is excellent in promoting dwarfing of lawn grass, making the stem sturdy, increasing the leaf count, and improving greenness, is provided.An agent for improving lawn grass quality containing, as active ingredients, 5-aminolevulinic acid represented by formula (1):R2R1NCH2COCH2CH2COR3  (1)wherein R1 and R2 each independently represent a hydrogen atom, an alkyl group, an acyl group, an alkoxycarbonyl group, an aryl group or an aralkyl group; and R3 represents a hydroxyl group, an alkoxy group, an acyloxy group, an alkoxycarbonyloxy group, an aryloxy group, an aralkyloxy group or an amino group,or a derivative thereof or a salt of the acid or the derivative, and a gibberellin biosynthesis inhibitor.

The invention relates to a rice flowering phase control protein and application thereof in breeding. An amino acid sequence of an OsLFT1 protein, an OsLFT1 nucleotide sequence encoding the OsLFT1 protein and a nucleotide sequence of an OsLFT1 gene promoter are included. For the first time, the rice OsLFT1 gene is cloned, an OsLFT1 overexpression plant expression vector is constructed, the OsLFT1 promoter starts the plant expression vector of a GUS report gene, OsLFT1 overexpression is created, the OsLFT1 promoter starts a rice transgenic line of the GUS report gene, a dwarf and later-floweringrice breeding material is obtained, and the function of OsLFT1 in rice is researched deeply. According to the cloned OsLFT1 gene and the created dwarf rice breeding material with the changing flowering stage, the rice anti-lodging capability is improved in rice dwarfing breeding; for rice variety breeding in different growing periods, the problem that the hybridized rice flowering stage is sterile is solved, and the protein has the wide application prospect in the aspects of increasing the rice yield and improving the rice quality.

The invention provides a methods and materials for producing and selecting plants with at least one dwarfing-associated phenotype. The methods and materials relate to altering the expression, or activity, of an ARF3 poypeptide in the plant, and selecting plants with altered the expression, or activity, of an ARF3 poypeptide. The invention also provides plants produced or selected by the methods. The methods also involve crossing plants of the invention with other plants to produce further plants with at least one dwarfing-associated phenotype.

The present inventors presumed that the sd1 gene was the C20 oxidase gene, and isolated and identified the rice counterpart of Arbidopsis C20 oxidase gene. As a result, it was revealed that the rice sd1 gene encodes a novel C20 oxidase. Further studies showed that mutation in this gene led to plant semidwarfing. Utilization of the plant sd1 gene is expected to increase the yield of plants, particularly useful agricultural crops and ornamental plants, to add aesthetic value to ornamental plants through dwarfing, and also to increase the yield and efficient breeding of dwarfed plants by marker selection.

The invention discloses a molecular marker related to dwarfing of corn plant. According to the invention, a mutant e975 related to dwarfing of corn plant height is screened, although the section number of corn is obviously reduced, the height of the corn is obviously reduced, and the ear length of the corn is obviously reduced, the yield of the e975 is not obviously different from that of a wild type. Gene localization shows that the first exon of the Zm00001d027807 gene of the mutant has changed from G to A, so that non-polar glycine is changed into alkaline arginine. For the variation, a corresponding molecular marker primer (SEQ ID NO.3-4) is developed, and after PCR amplification, Sanger sequencing is utilized for genotype interpretation, so that the wild genotype and the mutant genotype of the filial generation contain corn dwarfing gene Zm00001d027807 can be identified. The molecular marker is beneficial to auxiliary breeding of new corn dwarfing varieties.

The invention belongs to the technical field of the agricultural science, and discloses a method for constructing plant dwarfing and early flowering by using CRY1G380R. According to the method, with site-directed mutagenesis of conservative sites of plant cryptochrome CRY1, the constitutive dwarf of the plant can be generated, ie., the light quality and the light intensity generate light influence on the plant dwarfing development; with the method, the plant can significantly and early flowers, and the early flowering phenotype does not depend on photoperiod conditions of plant growing.