1054 results about "Japonica rice" patented technology

The invention discloses a method for acquiring an aromatic rice strain by targeting a Badh2 gene via CRISPR/Cas9 gene editing technology. The method comprises the following steps: separately targeting sequences, recognizable by Cas9, of every exon and intron of an aromatic gene by using the CRISPR/Cas9 gene editing technology and then cutting a genomic DNA sequence to initiate DNA restoration so as to obtain an afunctional Badh2 gene; with the callus of Indica rice, japonica rice or glutinous rice as a receptor material of genetic transformation and an mature embryo, young ear, ovary or the like as explant, carrying out induction so as to obtain a diploid callus, introducing a targeting vector into cells of the callus by using an Agrobacterium mediated transformation method, screening and identifying positive plants and separating the plants from a T1 colony so as to obtain an aromatic rice strain; and with anther, pollen, unfertilized ovary or the like as explant, carrying out induction so as to obtain a haploid callus, introducing the targeting vector into cells of the callus by using the Agrobacterium mediated transformation method, screening a positive callus, reduplicating the positive callus by using colchicine, carrying out differentiation to form seedlings and identifying genetic transformation positive plants so as to eventually obtain the aromatic rice strain.

The present invention relates to a site-directed knockout system for a rice TMS10 gene, and applications thereof. The site-directed knockdown system comprises a CRISPR/Cas9 system and a sgRNA target, wherein the sgRNA target is the sequence containing PAM or NGG in a rice male sterile gene TMS10, the CRISPR/Cas9 system is CC-TMS10-1, and the CC-TMS10-1 target sequence is a SEQ ID NO.1 sequence from site 2281 to site 2299. The applications comprise carrying out targeted knockout on the TMS10 genes of different japonica rice varieties and different Indica rice varieties by separately using CC-TMS10-1, wherein the experiment results prove that the heterozygous mutation transformation plant produced through the site-directed knockout induction in different rice varieties shows the temperature sensitive male sterility characteristic. According to the present invention, the site-directed knockout system provides the efficient breeding method for the creation of the temperature sensitive male sterile line germplasm resources based on the rice temperature-sensitive male sterile gene TMS10 and the rice hybridization breeding.

The invention discloses health-care coarse-grain flour which is prepared from the following raw materials in parts by weight: (1) coarse cereals: 60-70 parts of wheat, 4-6 parts of corn, 4-6 parts of polished round-grained rice, and 4-6 parts of barley; (2) auxiliary raw materials: 0.2-1 parts of walnut, 0.1-0.8 parts of cattle blood, 0.15-0.6 parts of goose blood, 0.1-0.6 parts of yam, and 0.1-0.8 parts of endodermis of chicken gizzard; meanwhile, the auxiliary raw materials of the health-care coarse-grain flour disclosed by the invention further comprise0.1-0.8 parts of pig blood, 0.1-0.6 parts of edible fungus, 0.2-1 parts of peanuts, and 0.2-1 parts of cassia seeds; in addition, the coarse-grain flour also can comprises 8-12 parts of one of tapioca, broad bean meal, peameal, soybean meal and millet or sorghum flour, or an arbitrary mixture thereof. The invention discloses a process for preparing the health-care coarse-grain flour. Meanwhile, the invention also discloses a health-care coarse-grain dried noodle prepared from the health-care coarse-grain flour above and a preparation process thereof.

The invention discloses nutritional cereal meal with a spleen tonifying effect and two preparation methods thereof according to GB10769-2010. The nutritional cereal meal with the spleen tonifying effect has the advantages that Codonopsis pilosula is added to the cereal meal so as to benefit the nourishing of human body spleen yang, tuckahoe and semen coicis are added so as to benefit the induction of diuresis and clearing of damp in human bodies, Chinese yam, lotus seeds and white hyacinth beans are added so as to benefit the nourishing of human body spleen yin, and gorgon fruit is added so as to play roles of inducing astringency and solidifying spleen yang. The tuckahoe, the semen coicis, the Chinese yam, the lotus seeds, the white hyacinth beans, the gorgon fruit and the fructus amomi are medical and edible food and can enhance the transportation and transformation of spleen qi in human bodies through long-term taking. Rice comprises tax rice, polished round-grained rice and sticky rice, so that the nutritional cereal meal is relatively comprehensive in nutrition and is easy to absorb by crowds with different physiques; as a certain amount of sticky rice is added, the nutritional cereal meal has certain sticky property, the gloss of the appearance of the nutritional cereal meal is improved, and the spleen tonifying and stomach nourishing effects of the nutritional cereal meal are improved. The nutritional cereal meal with the spleen tonifying effect is suitable for infant cereal accessory food eaten by infants at the age of above six months and children.

The invention discloses a five-organ-nourishing nutrient health rice and a preparation method thereof. The five-organ-nourishing nutrient health rice is prepared from the raw materials of japonica rice, buckwheat, chestnut cores, black sesame, mutton, naseberry, almonds, fish maw, balsam pear power, peperomia dindygulensis miq, shaddock peels, prepared rhizoma polygonati and the like. The five-organ-nourishing nutrient health rice provided by the invention has the benefits of having a unique special flavour, being rich in nutrient substances such as soybean dietary fiber and soy proteins, has the effects of clearing away heat and toxic materials, eliminating phlegm and draining pus, relax bowel to relieve constipation, nourishing five organs and dispelling wind and eliminating dampness, can prevent diseases such as colon cancer, hypertension, hyperlipidemia, high cholesterol and diabetes mellitus.

The invention relates to a health-care red koji wine and a production process thereof. The wine comprises the following raw materials in percentage by weight: 11.5-12.5% of nonglutinous rice, 13-17% of sticky rice, 4.5-5.5% of Chinese herbal medicine, 4.5*10<-2> to 5.5*10<-2>% of yellow wine herbal medicine starter and white rhizopus medical powder, 1-2% of red yeast rice, 4.5*10<-3> to 6.5*10<-3>% of diastase, 1*10<-3> to 1.5*10<-3>% of acid protease and 65-68% of water, wherein the using amount ratio of the yellow wine herbal medicine starter to the white rhizopus medical powder is (1-2):1.The production process provided by the invention comprises the following working procedures of preparing raw materials; preparing a Lin-fan rice wine starter; primarily pickling the sticky rice; secondarily pickling the Chinese herbal medicine and the sticky rice; carrying out post-fermentation at low temperature; squeezing and filtering; decocting the wine and sterilizing; storing and ageing; blending; freezing and flocculating; filtering and filling, and the like; and the prepared health-care red koji wine has low alcoholic strength, salubrious, savoury and mellow taste, and has the effectsof tonifying Yang and replenishing the vital essence, enforcing spleen and nourishing kidney as well as bodybuilding and healthcare.

The invention relates to a rice anther culture improvement technology, and in particular relates to a method for improving the frequency of callus induction and plant differentiation rate in japonica rice anther culture. The method comprises the following steps that: 200g/667m<2> of 10% of paclobutrazol wettable powder and 50-60L of water are mixed for spraying treatment before sampling, the spraying of the paclobutrazol and low-temperature treatment cause multiple pollen cells in the anther to be in the later monokaryotic stage, novel cytokinin 4-FA is introduced into a callus inducing medium, under the synergistic action of the 4-FA, KT, and 2,4-D, division is easily started in the dark culture after inoculation, and the frequency of callus induction is greatly improved. By using the anther culture method, the frequency of callus induction in the japonica rice anther culture is obviously improved to exceed 85.0%. A differential medium comprises the following ingredients: an MS (Murashige and Skoog) basic culture medium, 0.5mg/L of NAA, 0.5mg/L of ZTO, 0.8mg/L of TDZ, 0.2mg/L of FA, 0.2mg/L of MET, 30g/L of saccharose, and 8g/L of agar powder; and after the novel hormones of ZT, 4-FA and TDZ are introduced, light cultivation is carried out, and plant differentiation rate can reach more than 86%.

The invention belongs to the agriculture technical field, used for rice high yield nutrition efficient utilization cultivation, in particular relates to a japonica rice nitrogen, phosphorus and potassium fertilizers applying method. The method includes the following steps: fertilizer applying total weight is determined; leaf colour is determined; breed and fringe type are determined; and corresponding fertilizer applying management is carried out according to the determined fertilizer applying total weight, basic soil fertility, leaf colour and fringe type. Compared with the local high yield fertilizer applying method, the japonica rice high yield 'three-factor' nutrition management method in the invention has the advantages that mean nitrogen fertilizer application rate of the 'three-factor' nutrition management method is reduced by 15-22%, yield is improved by 6.6-10.6%, agricultural utilization ratio of nitrogen fertilizer is improved by 47-53%, and production capacity of nitrogen fertilizer is improved by 30-33%.

The present invention provides polyploid rice selectively breeding and identifying method with polyploid meiosis stability (PMeS) gene. The rice selectively breeding process includes: selecting parents; hybridization or complex hybridization; back crossing; doubling chromosome; identifying polyploid; selecting bearing property; observing meiosis behavior; self-crossing to form stable line; and identifying high bearing property. The identifying indexes include: polyploid line of Asia cultivated indica type rice or japonica rice; bearing rate not lower than 65 %; normal meiosis behavior; normal pollen shape and physiological activity and normal dyed pollution with 0.1 5 I-KI not lower than 95 %; bearing rate of hybrid of PMeS polyploid rice and other polyploid rice variety over 70 % and that of PMeS polyploid rice and wild rice variety over 65 %. The present invention solves the problem of low bearing rate of polyploid rice and may be used in breeding polyploid rice variety.

The invention discloses a wheat composite flour and a preparation method thereof. The flour is prepared from the following raw materials in parts by weight: 200-300 parts of whole wheat flour, 10-15 parts of japonica rice, 5-8 parts of betel nut taro, 6-10 parts of sargassum fusiforme, 6-8 parts of balsam pear, 5-10 parts of green money willow, 2-3 parts of hairyvein agrimonia herb and bud, 1-2 parts of sunflower leaves, 1-2 parts of a snakegourd fruit powder, 2-3 parts of cactus flowers, 1-2 parts of ginseng flowers, 2-3 parts of folium mori, 1-2 parts of potentilla discolor bunge, 2-3 parts of pumpkin vines, 5-8 parts of tremella powder, 4-6 parts of lentil and 3-6 parts of cucumber seed powder. The flour provided by the invention contains nutritional ingredients such as the betel nut taro, the green money willow and the like and has the effects of enriching nutrition, tonifying and nourishing the kidney, strengthening the spleen, nourishing the stomach, and reducing blood pressure and cholesterol, and is beneficial to people's physical health; moreover, various beneficial ingredients of traditional Chinese medicines are added into the formula; the compatibility is reasonable, the efficiency of health protection is enhanced, the processed wheat flour food is enabled to have the functions of easy digestion and absorption, and the cholesterol and blood pressure are lowered.

The invention belongs to the technical field of biotype identification, in particular to a method for identifying indica type rice and japonica rice using rice grain by using a rice grain (rice) and inserting or deleting (InDel) a molecular marker. The method comprises the following steps of: extracting DNA from the rice grain, and comparing full-genome DNA sequences of indica type rice 93-11 andjaponica rice Nipponbare to obtain 40 pairs of specific InDel primers; and performing fragment amplification, electrophoretic separation and electrophoresis pattern analysis on the extracted DNA the in rice seed to identify the characteristics of the indica type rice and japonica rice of a rice sample. The method concretely comprises the following steps of: taking the DNA extracted from the rice grain as a template, and analyzing and counting molecular fingerprint patterns obtained on the basis of a polymerase chain reaction and agarose electrophoresis by using combination of the 40 pairs of specific InDel primers; and determining the characteristics of the indica type rice and japonica rice of the tested rice seed (sample) according to gene frequency (Fi or Fj) calculated by 93-11 genotype and japonica rice Nipponbare genotype molecular fingerprint on 40 InDel loci of the sample. The result can be obtained by only detecting the sample of one grain of seed, and the method is convenient and rapid, accurate in identification, and has good popularization and application prospect.

The invention pertains to the technical field of biological discrimination, in particular to a method for discriminating indica rice from japonica rice by utilizing molecular markers of rice DNA insertion or deletion. The method obtains 34 pairs of differential DNA primers designed by InDel differential fragment by making a comparison of whole genome sequence between indica rice cultivar 93-11 and japonica rice cultivar (Nipponbare). The DNA extraction, the amplification and ionophortic separation of DNA fragments and the analysis and calculation of an electrophoretogram of a target rice cultivar are carried out to discriminate indica rice from japonica rice. Precisely speaking, the 34 pairs of InDel primer groups are utilized and analysis and calculation are carried out according to a finger-print obtained on the basis of a polymerase chain reaction and vertical slab gel electrophoresis; the characteristics of indica rice or japonica rice of rice samples detected are worked out according to the average frequency (Fi or Fj) showing on indica rice alleles and japonica rice alleles on 34 InDel locuses. The invention has the advantages of simple, fast and convenient method, few samples for detection and correct discrimination results, thus having good prospect of promotion and application.

The invention discloses health-care dietary-therapy radix ginseng and hericium erinaceus natural delicacy rice porridge which is prepared from the following raw materials in parts by weight: 15 to 30 parts of rhizoma dioscoreae, 8 to 25 parts of liquorice root, 10 to 20 parts of radix ginseng, 10 to 20 parts of white hyacinth bean, 15 to 30 parts of poria cocos, 10 to 20 parts of lotus seed, 10 to 20 parts of semen coicis, 10 to 20 parts of fructus amomi, 8 to 30 parts of orange peel, 10 to 20 parts of radix platycodon, 5 to 15 parts of hericium erinaceus, 200 to 400 parts of oat and 400 to 600 parts of polished round-grained rice.

The invention relates to an application of a paddy rice temperature sensitive male sterility gene TMS10 and a fertility restoration method. The amino acid sequence of TMS10 is represented by SEQ ID No.1. A conventional method is adopted to knock, change or inhibit TMS10 gene to reduce the expression level of TMS10 gene in common paddy rice species so as to obtain paddy rice male sterility line. The prepared paddy rice sterility line does not have abnormal phenotype during the vegetative growth phase; anther development is abnormal during the reproductive stage; the expressions of the phenotype are the same in the mutants of non-glutinous rice and japonica rice; the paddy rice is sterile at an average temperature of 28 DEG C, and paddy rice is fertile at an average temperature of 22 DEG C. The temperature sensitive characteristic, which is utilized during the process of two line hybrid seed production, is widely used in agricultural production.

The invention discloses a method for breeding high-yield japonica rice species capable of efficiently preventing rice blast. The method comprises the following steps: (1) hybridizing a rice blast preventing parent with a high-yield parent to obtain a hybrid F1 generation, planting the hybrid F1 generation, and harvesting the F2 generation seeds; (2) sowing the F2 generation seeds in the planting area in a proper time, and selecting the single plants to obtain F3 generation plants; (3) mixing the selected F3 generation plants, threshing, sowing in a proper time, planting single plants, in the booting stage, using the rice blast mixed bacterium liquid in the area which is suitable for promotion of the species to carry out inoculation, and choosing the single plants to obtain the F4 generation plants; (4) choosing segregation generation more than F4 generation, choosing the plants, and obtaining the seeds of target species. According to the method, a rice blast inoculation technology and a high-yield species small area selecting technology are organically combined; the rice blast resisting gene and high-yield gene are effectively combined; the yield level and the resistance level are both enhanced, the vitality of the novel species is improved, and the rice blast resistant level of the novel species is effectively enhanced at the same time.

The invention relates to a solid beverage and a preparation method of the solid beverage, wherein the solid beverage consists of the following raw materials in parts by weight: 3-5 parts of yam, 3-5 part of lotus root starch, 2-4 parts of Chinese wolfberry fruit, 1-2 parts of raspberry, 3-5 parts of red jujube, 1-3 parts of sesame, 3-5 parts of sweetener, 4-6 parts of japonica rice and 3-5 parts of maltodextrin. The solid beverage prepared by the method has the effects of nourishing spleen and kidney and replenishing vital energy and blood, is suitable for people with deficiency of spleen and kidney and insufficiency of vital energy and blood, and can be taken as a beverage before or after a meal or between two meals.

The invention discloses an easily digestible health-care rice cake, which comprises japonica rice flour, chicken's gizzard-membrane, astragalus, liquoric root, dwarf lilyturf root, lotus seeds, solomon's seal and sea-buckthorn. The health-care rice cake belongs to a health-care food, and the digestion and absorption of the rice cake are promoted to a certain extent.