10225 results about "Insect pest" patented technology

DIG-3 Cry toxins, polynucleotides encoding such toxins, and transgenic plants that produce such toxins are useful to control insect pests.

The present invention relates to a novel strain of Bacillus sp. D747 (deposited as FERM BP-8234) and methods for controlling plant diseases and insect pests, comprising administering cultures of Bacillus sp. D747 (including the viable bacteria) or viable bacteria isolated by culturing, on the plant parts such as roots, stems, leaves, seeds, and the like, or in the culture soil.

Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: disclosed herein, (but not including the forward and reverse primers.) or active variants and fragments thereof, or complements thereof, wherein a decrease in expression of one or more of the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiments, the pest is D. virgifera virgifera, D. barberi, D. virgifera zeae, D. speciosa, D. speciosa, or D. undecimpunctata howardi. Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

Disclosed are methods for increasing the activity of B. thuringiensis delta -endotoxins against Coleopteran insect pests. Also disclosed are methods for mutagenizing nucleic acid sequences encoding these polypeptides, and increasing insect resistance in transgenic plants expressing these genes.

The subject invention pertains to materials and methods useful for monitoring and management of certain pests well as others biotic and abiotic factors. The invention is particularly well suited for the control of social insect pests, and particularly, termites. The invention concerns methods and apparatuses for monitoring pest activity and presenting a toxicant. The invention is useful as part of an Integrated Pest Management Program and can significantly enhance the efficacy, efficiency and convenience of the management program.

Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide expression cassettes and transformed plants, plant cells, and seeds. These compositions find use in methods for protecting a plant from a pest.

Compositions and methods for controlling plant pests are disclosed. In particular, novel nucleic acid sequences encoding modified Cry3A toxins having increased toxicity to corn rootworm are provided. By inserting a protease recognition site, such as cathepsin G, that is recognized by a gut protease of a target insect in at least one position of a Cry3A toxin a modified Cry3A toxin having significantly greater toxicity, particularly to western and northern corn rootworm is designed. Further, a method of making the modified Cry3A toxins and methods of using the modified cry3A nucleic acid sequences, for example in microorganisms to control insects or in transgenic plants to confer protection from insect damage, and a method of using the modified Cry3A toxins, and compositions and formulations comprising the modified Cry3A toxins, for example applying the modified Cry3A toxins or compositions or formulations to insect-infested areas, or to prophylactically treat insect-susceptible areas or plants to confer protection against the insect pests are disclosed.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The present invention relates to genetic control of infestation by insect pest species, particularly prevention and / or control of pest infestation of plants, using interfering ribonucleic acid (RNA) molecules. The invention provides transgenic plants that (i) express or are capable of expressing interfering RNAs of the invention and (ii) are resistant to infestation by insect pest species.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention relates to a composite microbial fertilizer for controlling crop diseases and insect pests, which contains composite microbial fungicide and is prepared by the following raw materials based on parts by weight: 10-35 parts of composite microbial fertilizer fungicide, 3-15 parts of composite microbial pesticide fungicide, 1-5 parts of insecticidal synergist, 1-5 parts of plant growth regulator, 20-60 parts of grass carbon, 5-15 parts of expanded chicken manure, 5-50 parts of inorganic fertilizer and 1-3 parts of binding agent, wherein the composite microbial fertilizer fungicide is prepared by bacillus megaterium and jelly-like bacillus; and the composite microbial pesticide fungicide is prepared by mixing bacillus thuringiensis and bacillus subtilis, adding sodium percarbonate type solid state oxygen-increasing agent for mixing and fermenting and finally absorbing by the grass carbon. The composite microbial fertilizer adopts strains with good suitability, and the strains are compounded according to a certain proportion to obtain the composite fungicide which has durable activity and stable performance; and the four beneficial strains are compatible with each other and performs the own functions, so that the multiple aims of resisting germs as well as diseases and insect pests, and increasing the crop yield can be achieved.

Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barberi, D. speciosa, or D. undecimpunctata howardi. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention discloses controlled-released pesticide fertilizer and preparing method thereof. The controlled-released pesticide fertilizer comprises, by mass, 0.001-15% of effective component A, 4%-45% of nitrogen, 1%-15% of phosphorus, 1%-25% of potassium, 0.1%-5% of microelement, 0.01%-10% of coagulator, 0.01%-10% of binder, 0.15-10% of surfactant and the balance packing. The preparing method of the controlled-released pesticide fertilizer comprises is simple and comprises the steps of preparing of liquid materials, preparing of powder materials and fluidized bed granulating. The controlled-released pesticide fertilizer can control the occurrence of damage caused by diseases, insect pests and weeds can be controlled in all the seasons of crop growth and meet the supply of nutrient substances needed in all the seasons of crop growth, production and use are safe, labor and cost are reduced, and the application amount and the use frequency of pesticides and the fertilizer in the period of crop growth can be reduced.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention discloses a compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and a preparation method of the compound microorganism liquid bacterial fertilizer and belongs to the technical field of microorganisms. The bacterial fertilizer comprises raw materials of, by weight, 10-20 parts of paprika powders, 10-20 parts of chilli powders, 5-15 parts of ginkgo leaf powders, 5-15 parts of chinaberry powders, 5-15 parts of lightyellow sophora root powders, 20-40 parts of mashed garlic, 40-80 parts of molasses, 4-8 parts of soybean meal, 5-15 parts of calcium sulfate, 5-15 parts of magnesium chloride, 5-10 parts of salt, 5-15 parts of photosynthetic bacterium strains, 5-15 parts of saccharomycete strains, 5-15 parts of lactic acid bacterium strains, 5-15 parts of streptomyces jingyangensis strains, 5-15 parts of bacillus subtilis strains and 800 parts of water. According to the compound microorganism liquid bacterial fertilizer, probiotic floras which are screened and are capable of coexisting are used for fermenting plant leaching agents of pepper, chilli, ginkgo leaves, chinaberry, lightyellow sophora roots and garlic, the plant leaching agents are capable of expelling and killing the insect pests and harmful bacteria, the compound microorganism liquid bacterial fertilizer has the advantages of being high in content of live bacteria of probiotics, high in activities, high in stability, small in using amount of plant materials, fast in acting effects, non-toxic and environment-friendly, and has wide application prospects in agricultural planting.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

Traditionally, the primary method for impacting insect pest populations is the application of broad-spectrum chemical insecticides. However, there is increasing concern about the environmental hazards associated with the production and use of synthetic chemical pesticides. Thus, there is substantial interest in developing alternative pesticides, including biological control of insect pests of agricultural significance using a microbial agent or another species of insect. The present invention provides compositions and methods for such biological control. Modified Cry3 pesticidal polypeptides, polynucleotides encoding such polypeptides, and methods of use are disclosed. The modified polynucleotides provided herein can be used to transform organisms and cells of hosts comprising plant, insects, and microorganisms. The expression of modified polypeptides can provide the host with improved insecticidal activity against one or more insect pathogens.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention discloses a pyrazolyl acrylonitrile compound with a novel structure. The structure of the compound is shown as the general formula I, and in the formula, the definitions of substituent groups are specified in the specification. The compound with the general formula I has excellent activity for killing insects and mites and can be used for preventing insect pest and mites.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The present invention provides a microbe composition, tobacco-dedicated biological organic fertilizer containing the composition, its usage method. The microbe composition comprises aztobacter sp., pseudomonas fluorescence, Bacillus megaterium, bacillus mucilaginosus and bacteria capable of preventing and treating plant disease and insect pests. The microbe composition is mixed according to proportion with oligochitosan, lignite, grass charcoal, and bean cake and fermented to make bacteria powder fertilizer, and further added with potassium-enriching shale, bentonite and water-keeping agent to make bacteria granule fertilizer. The tobacco-dedicated biological organic fertilizer contains the above-mentioned bacteria powder fertilizer and granule fertilizer which contains natural mineral and good organic substance, C / N value being large, which fit the growth rule of tobacco plant and promote potassium fertilizer efficiency and possesses long-lasting potassium-supply capability and provides effective ingredient for tobacco.