The invention discloses a method for rapidly screening microalgae
germplasm with high
grease content. The method comprises the following steps: sampling a
water sample, culturing and separating dominated
algae species; then, adopting an
enzyme-labeling instrument to detect a light absorption value of 490nm to rapidly reflect the growth condition of
algae; adopting a fat-soluble fluorescent dye
Nile red to dye microalgae; utilizing 485nm exciting light and 575nm dissipating light of the fluorescent
enzyme-labeling instrument to quantificationally detect the cytolipin content of the microalgae; and finally, confirming pure algal strains and storing the
algae species. The screening process is simple, convenient and high-efficient and can carry out large-scale, high-efficient and rapid separation of the microalgae
germplasm with high
grease content and high growth rate from a
natural water body while integrating the synchronous monitoring of the
biomass and the
grease content so that suitable algae species are separated by one step; the whole separation, screening and detection process can realize high-throughout screening without changing a culture container; the destination algal strains are obtained by one step through different detection programs, and the working efficiency of the screening is greatly improved.