The invention discloses a quantitative
fructose assay kit which comprises inorganic acid deproteinized extract A, inorganic base deproteinized extract B,
fructose calibration solution, a
reagent 1 containing 0.001-0.1mol / L
adenosine triphosphate
sodium salt, a
reagent 2 containing 1-100KU / L
hexokinase and 1-100KU /
L glucose-6-
phosphate dehydrogenase, and a
reagent 3 containing 0.001-0.1mol / L
nicotinamide adenine dinucleotide. The seminal
plasma fructose assay method comprises the following steps: respectively adding the reagent 1 and the reagent 2 to deproteinized seminal
plasma and the fructose calibration solution, and mixing uniformly; reacting at the temperature of 10-40 DEG C for 5-120 minutes, then reading the
absorbance respectively at the
wavelength of 280-400nm; adding the reagent 3 respectively, and mixing uniformly; reacting under the same conditions and reading the
absorbance; and calculating the difference between the
absorbance read at the first time and the absorbance read at the second time, and comparing or calculating the absorbance of a seminal
plasma specimen and the fructose calibration solution to obtain the concentration of the seminal plasma fructose. The kit and the method can be used for
quantitative determination of fructose in sera, plasma,
body fluid, food and
solid extracting solution, the methodology is special, unique, clean and environment-friendly, manual operation and automatic batch
assay can be realized, and the kit and the method are easy to popularize and apply clinically.