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Creatine kinase isoenzyme detection reagent kit

A detection kit and creatine kinase technology, applied in the field of medical testing, can solve the problems of unreliable measurement results, low reagent stability, large measurement errors, etc., achieve good clinical application value, improve bottle opening stability, improve The effect of shelf life stability

Inactive Publication Date: 2019-06-04
曾宪亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently, immunosuppression is the most commonly used clinically. It may be that there are giant CK, CK-BB, AK and other influencing reagents in the serum. The subunit participates in the enzyme reaction with the B subunit at the same time, and because the calculation result is multiplied by 2, the error is further enlarged; moreover, the temperature drift of the detection instrument and the interference of the fat-turbid specimen will cause a large measurement error, so that the measurement result Unreliable, and the stability of the reagents is not high

Method used

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  • Creatine kinase isoenzyme detection reagent kit

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Embodiment 1

[0037] A creatine kinase isoenzyme detection kit, comprising reagent R1 and reagent R2; wherein reagent R1 is made up of components of the following concentrations: 10g / L biological buffer (imidazole buffer, pH value is 7), 3g / L magnesium acetate, 6.2g / L N-acetylcysteine, 2KU / L hexokinase, 1.8g / L surfactant (alkyl glucoside) and 2mL / L creatine kinase Isozyme antibody (anti-human CK-M monoclonal antibody);

[0038] Reagent R2 is composed of components at the following concentrations: 20g / L creatine phosphate, 1.5g / L adenosine diphosphate and 15KU / L glucose-6-phosphate dehydrogenase.

Embodiment 2

[0040] A creatine kinase isoenzyme detection kit, comprising reagent R1 and reagent R2; wherein reagent R1 is made up of components of the following concentrations: 13.8g / L of biological buffer (imidazole buffer, pH value is 6) , 1.25g / L magnesium acetate, 5g / L N-acetylcysteine, 1.8KU / L hexokinase, 2.5g / L surfactant (propylene oxide-ethylene oxide block copolymerization substance) and 2.5mL / L creatine kinase isoenzyme antibody (anti-human CK-M monoclonal antibody);

[0041] Reagent R2 is composed of components at the following concentrations: 10g / L creatine phosphate, 5g / L adenosine diphosphate and 8KU / L glucose-6-phosphate dehydrogenase.

Embodiment 3

[0043] A creatine kinase isoenzyme detection kit, comprising reagent R1 and reagent R2; wherein reagent R1 is made up of components of the following concentrations: 15.2g / L of biological buffer (imidazole buffer, pH 6.7) , 1.24g / L magnesium acetate, 8.1g / L N-acetylcysteine, 1KU / L hexokinase, 1g / L surfactant (alkyl glucoside 0.5+ propylene oxide-epoxy Ethane block copolymer 0.5) and 1.2mL / L creatine kinase isoenzyme antibody (anti-human CK-M monoclonal antibody);

[0044] Reagent R2 is composed of components at the following concentrations: 15g / L creatine phosphate, 2.5g / L adenosine diphosphate and 12KU / L glucose-6-phosphate dehydrogenase.

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Abstract

The invention is suitable for the technical field of medical inspection, and provides a creatine kinase isoenzyme detection reagent kit. The reagent kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises the following components of 10-20g / L of biologic buffering agents, 1-3g / L of magnesium acetate, 5-10g / L of N-broncholysin, 0.5-2KU / L of hexokinase, 0.5-2.5g / L of a surfactant and 0.8-2.5mL / L of creatine kinase isoenzyme antibodies, and the reagent R2 comprises the following components of 5-20g / L of creatine phosphate, 1.5-5g / L of adenosine diphosphate and 8-18KU / L of glucose-6-phosphate dehydrogenase. The reagent kit disclosed by the invention can be applied to detection of the activity of the creatine kinase isoenzymes in serum of human bodies, the reagent stabilityis high, and the detection result is high in precision and accuracy.

Description

technical field [0001] The invention belongs to the technical field of medical testing, in particular to a creatine kinase isoenzyme detection kit. Background technique [0002] Creatine kinase MB isoenzyme (CK-MB) is a hybrid dimer composed of M and B subunits, mainly present in the outer plasma layer of cardiomyocytes, and is the most specific enzyme in the myocardial enzyme spectrum. [0003] Most of normal human serum is the activity of CK-MM (the main active part of creatine kinase), only a small amount of CK-MB, CK-MB accounts for less than 5% of the total activity of CK, and the absolute value does not exceed 25U / L, and The measured value of CK-MB increased with the measured value of CK. When myocardial cells are damaged or necrotic due to inflammation or infarction, a large amount of CK is released into the blood. At this time, CK-MB in the serum accounts for more than 5% of the total CK activity, which is positive, and the highest value reaches 12%-38%. 3 hours af...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/48C12Q1/32
Inventor 曾宪亮
Owner 曾宪亮
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