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Serum AFU detection kit

A technology of serum fucosidase and detection kit, which is applied in the field of medical testing and determination, can solve the problems of unstable substrate and insufficient sensitivity, and achieve the effect of simple method and easy operation

Active Publication Date: 2015-01-07
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the substrates used in this method are generally unstable and the sensitivity is not high enough.
Based on this problem, the present invention provides a method for preparing a high-performance serum fucosidase detection kit, and perfectly solves the problems of substrate stability and sensitivity

Method used

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  • Serum AFU detection kit
  • Serum AFU detection kit
  • Serum AFU detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]

[0034]

[0035] The preparation method of Reagent 1 and Reagent 2 is a conventional method, that is, the components of Reagent 1 and Reagent 2 are added to distilled water and then mixed and stirred evenly.

Embodiment 2

[0037]

[0038] The preparation method of the kit in Example 2 is the same as in Example 1.

Embodiment 3

[0040]

[0041]

[0042] The preparation method of the kit of Comparative Example 3 is the same as that of Example 2, but the substrate protection system is removed.

[0043] The performances of Example 2 of the present invention and Comparative Example 3 will be described below in combination with tables and drawings.

[0044] 1. Substrate stability

[0045]The accelerated stability test at 37°C shows (the liquid reagent is prepared for the accelerated stability experiment at 37°C, and the accelerated stability test at 37°C for 7 days can simulate reality and be stored at 4-8°C for 1 year.), the prepared kit in Example 2 has good stability, The substrate was not degraded, and the blank absorbance increased little; while the reagent blank of the kit prepared in Comparative Example 3 increased significantly, showing that under the condition of no substrate protection system, the degradation of the substrate led to a significant increase of the blank. The results are show...

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Abstract

The invention relates to a serum AFU detection kit. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 comprises 50-500 mmol / L of a buffer solution (pH 4.0-4.5), 1-50 g / L of a surfactant, 1-20 g / L of an anti-interference agent and 0.1-100 g / L of a preservative; the reagent 2 comprises 50-500 mmol / L of a buffer solution (pH 7.0-7.5), 2-60 g / L of glucose, 1-20 KU / L of hexokinase, 1-20 KU / L of glucose-6-phosphate dehydrogenase, 0.3-5 g / L of magnesium sulfate, 0.2-5 g / L of a substrate, 1-100 mmol / L of a stabilizer, 0.1-40 g / L of a protective agent and 0.1-100 g / L of a preservative. With the adoption of the kit, problems of the poor substrate stability, the poor anti-interference capacity and the lower sensitivity which exist universally are solved.

Description

technical field [0001] The invention belongs to the technical field of medical examination and measurement, and in particular relates to a serum fucosidase detection kit. Background technique [0002] α-L-Fucosidase (α-L-Fucosidase, AFU), is a lysosomal acid hydrolase, widely distributed in various tissues, cells and body fluids in the human body, especially active in tissues such as liver and kidney higher. As a lysosomal acid hydrolase, AFU can hydrolyze fucose-containing lipids, mucins and mucopolysaccharides. AFU reagents use this characteristic to detect their activity. In 1977, Bauer et al. observed in animal experiments that AFU activity in Morris rat liver cancer tissue was 7 times higher than that in normal liver, and it was associated with tumors for a long time. In 1984, Deugnier et al. found that AFU was elevated in the serum of patients with hepatocellular carcinoma, and proposed AFU as a new marker enzyme for the diagnosis of liver cancer, and subsequent stud...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52
CPCG01N33/52C12Q1/00
Inventor 邹炳德邹继华黎鹏贾江花
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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