1207 results about "Ophiocordyceps sinensis" patented technology

The invention relates to a qualitative and quantitative analysis method for polyoses, which comprises the following steps: (1) the zymohydrolysis and the verification of the polyoses: measuring the content of a polyoses water solution by a colorimetric method; (2) comparing the change of a polyoses mapping before and after zymohydrolysis by HPSEC-ELSD analysis; (3) saccharide ingredient direct HPLC analysis of a polyoses zymohydrolysis solution or HPLC analysis after pre-column derivatization: identifying polyoses mapping and chromatogram peaks by taking a standard monosaccharose, a uronic acid, a disaccharide, and the like as contrasts and taking a chromatogram peak mass-to-charge ratio as reference, and using stable characteristic sections as a quantitative analysis index; and (4) realizing the qualitative and quantitative analysis of the polyoses by establishing polyoses zymohydrolysis responding characteristics and polyoses zymohydrolysis mapping which are based on structural information through the independent or combining application of the steps. The qualitative and quantitative analysis method can be used for the identification and quantitative measurement of the polyoses of traditional Chinese medicine, such as panax, panax pseudoginseng, gen-seng, aweto, cordyceps, ganoderma lucidum, fomes japonica, milk veteh, angelica, and the like and provides an effective method for controlling the quality of the polyoses and the products thereof.

A dietary supplement is created, comprised of material from the following nutrients, vitamins, herbs, minerals, and food and plant substances and food and plant derivatives: lycopene, vitamin E, selenium, green tea, coenzyme Q10, garlic, folic acid, vitamin C, curcumin, seaweed, Cordyceps sinsensis mushroom, Lentinus edodes (shiitake) mushroom, and Ganoderma lucidum (reishi) mushroom. The composition is administered orally for individuals who wish to reduce their risk of disease, particularly cancer-risk.

The invention mainly utilizes different grains or forage such as coix seeds, rice, wheat, oat, etc. as the substrate of the solid state fermentation of cordyceps or ganoderma lucidum. After solid-state cultivation, fermentation production containing high polysaccharide can be gained; the fermentation production is dried and milled into powder. In addition, farina and flavoring agent can be added, then cordyceps or ganoderma lucidum coix seed powder, rice powder, wheat powder, or oat powder with rich health protection components such as dietary fiber and polysaccharide can be produced, thereby being convenient for consumers to brew and drink, or forage contained cordyceps or ganoderma lucidum for fed animals to eat can be produced.

This invention describes the large-scale fermentation and processing of Chinese caterpillar fungus. In this invention, hirsutella sinensis strains are used as the fermentation strain and cultivated in the inclined plate and shaking-bottle. 3-5% of potato, 0.5-2% of soybean powders, 0.01-0.02% of soifnum muicfun, 0.01-0.02% of Chinese yam, 0.5-2% of protein, 0.5-5% of glucose, a small amount of dipotassium hydrogen phosphorus and magnesium sulfate, and water culture medium are successively directed into all levels of fermenter for fermentation and processing. The yield of the fermented product is 20% higher than that in the present processes, the fermentation periodic time is 12 days shorter, the cost is 20% lower and the pollution rate is 2% lower. Through the DNA molecular sequence examination by the institute of microbes, Chinese Academy of Sciences, the constitution of the fermented product is the same with that of natural Chinese caterpillar fungus, and the content of cordycepin, adenosine, Chinese caterpillar fungus polyoses, sterol, biological base, amino acids and trace elements is much higher than that in natural Chinese caterpillar fungus. The pharmacological action of the fermented product is better than that of natural Chinese caterpillar fungus.

The invention provides a method of full-artificial culturing Cordyceps sinensis, including sampling, strain separating, host insect collecting, breeding and feeding, infecting and continuing feeding, on artificial condition, largely culturing Hirsutella sinensis; feeding host insects in an scale room at low altitude, completing the infection of Hirsutella sinensis to the host insects and sexuality of Cordyceps sinensis. By artificial culture, the host grubs are big and good-quality, and the success rate of infection is high, the infection time is short, the quality is stable and controllable, and the pesticide effect test fully indicates that the pesticide effect of the produced Cordyceps sinensis is equivalent to that of the natural Cordyceps sinensis and it can overcomes the disadvantages of long growing time of natural Cordyceps sinensis and low natural infection ratio, and implements the industrialized production of Cordyceps sinensis.

The invention relates to tea drink with main materials of purple potato leaves and blueberry leaves. The tea drink is precisely prepared by inactivating, kneading and drying 10-50 parts by weight of purple potato leaves, 10-50 parts by weight of blueberry leaves, and the materials of purple potato flower, purple potato stem, blueberry flower and the natural material of cordyceps militaris, and thelike. The purple potato leaves, blueberry leaves, purple potato flower, purple potato stem, blueberry flower and the natural material of cordyceps militaris are rich in vitamin C. The anthocyanin ingredients contained in the blueberry leaves can delay aging and improve the immunity of a human body. The tea drink has obvious effect and favorable stability without toxicity. After drunk by most people, the tea drink is proved to have rich nutrition, comfortable mouth feeling, and convenient drinking and be easily absorbed. The tea drink has the characteristics of specific prescription, reasonable formula, simple process, strong health-care function, safety and non-side effect, is good for both children and adults, can be drunk for a long time and has good health-care effect.

The invention discloses a culture medium to ferment and manufacture Cordyceps sinensis mycelium, which consists of bevel culture medium and fermentation culture medium, wherein different culture mediums are adopted in different stages to ensure plain growth of the Cordyceps sinensis and equal quality of fermented and natural Cordyceps sinensis, which modifies the product quality and improves the production with certain effect. The invention supports the character of nourishment with natural Cordyceps sinensis, which is fit for industrial manufacturing need.

A manufacturing method for cordyceps sinensis (Berk.) sacc polysaccharide relates to the technical field of food microorganism application, and comprises the following steps: weighing raw materials, wherein rice bran is 1-3 g per 100 ml, bran is 1-3 g per 100 ml, potassium dihydrogen phosphate is 0.09-0.18 g per 100 ml, and magnesium sulphate is 0.04-0.08 g per 100 ml; material filling with natural pH after adding required water, and sterilizing and cooking at the temperature of 121 DEG C for 30 min; inoculating cordyceps sinensis (Berk.) sacc CCTCCM2013285 liquid seed after cooling, wherein the inoculation amount is 8-10%, the fermentation temperature is 23-28 DEG C, and the fermentation time is 4-7 d; respectively obtaining exopolysaccharides and mycelia polysaccharide after conducting deproteinization, alcohol precipitation, centrifugal separation, and vacuum drying on centrifugal separation liquid extracted through breaking cell wall hot water of fermentation material centrifugal separation liquid and hypha. The method better realizes the purpose that rice bran and bran are efficiently and high-valuedly transformed to cordyceps sinensis (Berk.) sacc polysaccharide after being subjected to liquid state fermentation.

A technique for culturing cordyceps by insect includes such steps as collecting fresh wild cordyceps, tissue separation to obtain its bacterial strain, reproduction, culturing slant strain and liquidbacteria, choosing host insect or host insect surrogate, spraying bacterial liquid for infecting the host insect, and culturing in the condition stimulating the wild environment. Its advantages are low cost and high effect.

The invention discloses a corayceps militaris industrial cultivation method. The corayceps militaris is cultivated in an industrial way by strain preparation, blending, bottling, sterilizing, inoculation, culture, germination and collection; fimilar artificial substitute materials are used as the culture materials, which reduce manufacture cost. In addition, the corayceps militaris obtained by the invention has the characteristics of good quality stability and high biological percent conversion, etc.

The invention discloses a method for extracting and purifying polysaccharide from a fermented mycelium of Cordyceps sinensis. The method comprises the following steps of: using the fermented mycelium of Cordyceps sinensis as a raw material to produce crude polysaccharide of the Cordyceps sinensis via degreasing, aqueous extraction, concentration and alcohol precipitation; then, redissolving the crude polysaccharide into distilled water, deproteinizing the crude polysaccharide by Sevag, decoloring the deproteinized crude polysaccharide by H2O2, dialyzing the decolored crude polysaccharide, and precipitating the dialyzed crude polysaccharide by alcohol to produce primarily purified polysaccharide; finally, purifying the primarily purified polysaccharide by ion-exchange chromatography, collecting different produced compositions stepwisely, dialyzing the compositions, precipitating the dialyzed compositions by alcohol, drying the precipitated compositions to produce the polysaccharide of the Cordyceps sinensis after the precipitated compositions are orderly washed by absolute ethyl alcohol, acetone, aether and so on. In the produced polysaccharide of the Cordyceps sinensis, MPS2 is identified to be homogeneous polysaccharide by ultraviolet absorption spectrum and gel chromatography, with high content of total sugar. The method has the advantages of simple and convenient extraction steps and low cost.

A functional health-care beverage for elongating life of man and taking care of the health of man is prepared from the grains wine and 43 Chinese-medicinal materials including ginseng, pilose antler, genoderma, cordyceps, etc.

The invention provides a method for increasing the yield of natural cordyceps sinensis. The method comprises the following steps of (1) selecting a base for increasing the yield of natural cordyceps sinensis; (2) placing a trapping lamp; (3) preparing trapping liquid; (4) screening and preparing culture medium of hepialidae hirsutella sinensis and paecilomyces hepialid; and (5) controlling yield increase conditions. According to the method, at the adult stage, a high pressure mercury vapor lamp is used for trapping, so the imagoes of hepialus armoricanus around the lamp fly to the base, and the egg-laying amount is increased; ethanol, vinegar and sugar solution are sprayed on meadows beneficial to larva growth, so the larvae gather together; and the hepialidae hirsutella sinensis and the liquefied paecilomyces hepialid are sprayed on the gathering area of larvae to raise the infection rate of the larvae of hepialus armoricanus, so the aim of increasing the yield of cordyceps sinensis is achieved and the industrialized production is realized.

The invention relates to a fermented product which is obtained by fermenting valuable medicinal edible fungi, especially aweto, and a preparation method thereof. Raw materials for preparing the active component of the product comprises 1-2 loops of aweto strains or aweto, sea buckthorn, wild peaches, mulberries, dark plums, oranges, etc. The preparation method comprises the following steps of: culture preparation; aweto ferment raw juice preparation; fruit ferment raw juice preparation; probiotics fermentation. The beneficial effect of the invention is that: functional components are accumulated by aweto fermentation so as to achieve the purposes of multi-efficacy of aweto of lung invigoration and kidney nourishment, immunoenhancement, antioxidation, radiation resistance, and the like.

A method for artificially culturing north cordyceps to increase the contents of its physiological active components (cordyceptic acid, cordycepin and cordyceptic polyose) includes such steps as washing fresh wild north cordyceps, section, regulating pH of culture medium to 6-7, sterilizing, shaping slant, slant culturing at 20-25 deg.C for 7-10 days, regulating pH of liquid culture medium to 6-7,sterilizing, inoculating, culturing, preparing solid culture medium from rice and sorghum grains, inoculating, and culturing at 25-29 deg.C for 40-60 days.

The invention discloses rhodiola crenulata essence with skin softening and anti-wrinkle effects. The rhodiola crenulata essence is mainly prepared from rhodiola crenulata extract, ganoderma lucidum extract, cordyceps sinensis extract, saffron extract, saussurea involucrata extract and corresponding adjuvants. The rhodiola crenulata essence, which is prepared by effectively compounding the natural-activity extracts of rhodiola crenulata, ganoderma lucidum, cordyceps sinensis, saffron, saussurea involucrata and the like together and mixing with the adjuvants, can promote skin metabolism, enhance activity, eliminate injuries caused by oxygen free radicals, improve the skin elasticity, enable the skin to keep tight, young and smooth, and realize effects of excellent deep-moisturizing, whitening, freckle removing and skin softening, thus slowing down the aging of organisms.

An antitussive cream for treating chronic tracheitis, infantal pneumonia, cough, etc. is prepared from 18 Chinese medicinal materials including cordyceps, gecko, ginseng, tangerine peel, etc. throughpretreating of raw materials, mixing them, decocting and shaping. Its advantage is high curative effect.

The invention belongs to the technical field of fungus culture, particularly to a method for culturing mycelia and sporocarps of aweto, which solves the problem that artificial aweto in the prior art can not be cultured in a large scale. The method comprises the following steps of: selecting cephalosporium acremonium and Isaria cicadae; preparing a cephalosporium acremonium liquid strain and an Isaria cicadae liquid strain; preparing a solid culture medium from corn grit, soybean flour, wheat grains, sorghum grains, silkworm chrysalis meal, whole chicken eggs, black bean coarse powder containing hulls, degreased linseed oil and a nutrient solution, wherein the nutrient solution comprises chicken blood, pine needles, carrots, black beans and cattle bone; evenly mixing, and inoculating; and culturing to obtain the sporocarps and the mycelia. The detection indicates that the sporocarps contain at least 0.02% of adenosine and at least 0.1% of cordycepin, and the mycelia contain at least 0.055% of adenosine; the finger-print test result indicates that the degree of similarity of the sporophores and the mycelia is greater than 97%; and acute toxicity experiments and long-term toxicity experiments indicate that the sporophores and the mycelia have no toxicity and can be used as substitutes of natural aweto.