416 results about "Inonotus obliquus" patented technology

The invention belongs to the field of biofermentation engineering. A pharmaceutical medium and a fungal strain which have blood sugar lowering efficacy are used as bidirectional fermentation raw materials, wherein the pharmaceutical medium is mainly composed of the following raw materials: cornel, gynostemma, momordica grosvenori, winged euony twigs, balsam pear and polyrhachis vicina; the fungalstrain is composed of the following strains: inonotus obliquus, cordyceps, phellinus igniarius, polystictus versicolor, and grifola frondosa; and bi-directional multi-fungi fermentation is carried out between multiple edible and pharmaceutical fungi and Chinese herbal medicines by a bidirectional fermentation process, thus organisms in two kingdoms or three kingdoms are organically combined to obtain an entirely new blood-sugar-lowering pharmaceutical mycoplasm product. The obtained product can generate 1+1>2 physiological function efficiency for hypoimmunity, has the actions of obviously lowering blood sugar but not increasing insulin concentration, has significant positive effects on helping treating and controlling diabetic complications, and can be used as an oral medicament for preventing and treating diabetes or a health-care product for adjusting blood sugar.

The invention discloses a culture medium and method for submerged fermentation of inonotus obliquus, relating to a culture medium and method for microbial submerged fermentation, and solving the problems of low yield of traditional inonotus obliquus submerged fermentation mycelia and low output of intracellular polysaccharide and extracellular polysaccharide of the inonotus obliquus as the activeingredient for the submerged fermentation. The culture medium for submerged fermentation is prepared from the following components: carboxymethyl cellulose, glucose, soybean meal, yeast extract, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 and the balance of water. The method for the submerged fermentation comprises the following steps of: (1) preparing a culture medium for the submerged fermentation of inonotus obliquus; (2) inoculating the inonotus obliquus; and (3) carrying out submerged fermentation at two stages. By means of the culture medium and the method for the submerged fermentation of the inonotus obliquus in the invention, higher mycelium yield and intracellular and extracellular polysaccharides can be obtained. The mycelia polysaccharide obtained by the invention is 1.4 times higher than that of wild fruiting bodies, and the content of other active ingredients is higher than or equal to that of the wild fruiting bodies. The culture medium and method provided by the invention can be used for providing a rapid and abundant seed liquid for the large-scale artificial culture of the inonotus obliquus.

The invention relates to the technical field of fermentation engineering, and discloses a submerged fermentation technology of producing immunological enhancement active materials of inonotus obliquus. The submerged fermentation technology of producing the immunological enhancement active materials of the inonotus obliquus comprises the main steps of culture activation, culture liquid cultivation, submerged fermentation and preparation of the immunological enhancement active materials of the inonotus obliquus and the like. The optimized submerged fermentation technology of producing the immunological enhancement active materials of the inonotus obliquus is high in content of the obtained immunological enhancement active materials, and good in activity of the obtained immunological enhancement active materials, and the obtained immunological enhancement active materials are beneficial for industrial application.

The invention relates to a method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus. The method mainly comprises the following steps: high-pressure treatment, ethanol extraction, concentration, centrifugation, deionized water extraction, concentration, ethanol precipitation, separation, purification, freezing and drying. Compared with the conventional extraction method, the provided method has the biggest difference in that high-pressure treatment is carried out to the raw material for preliminary treatment under the appropriate time condition, then an extract containing steroids is obtained by an ethanol extraction method, the steroidal compounds are purified by a water washing method and a centrifugation method, extracts of polysaccharides and polyphenols are obtained by a water extraction method, and then the polysaccharides and polyphenols are separated through ethanol precipitation. The tissue structure of inonotus obliquus changes due to high-pressure treatment, therefore, the yield of the steroidal compounds, polysaccharides and polyphenols is high, the molecular structure of active components in inonotus obliquus changes under high pressure, and the study verifies that the activity of inonotus obliquus can be enhanced.

The invention discloses an active ferment with a high SOD content and a preparation method thereof. The preparation method comprises the following steps: (a) cutting different fruits and vegetables into slices, grinding the slices, and adding white sugar; (b) pulping edible fungus, extracting cordyceps militaris sporocarp and hericium erinaceus sporocarp to obtain extracts, and mixing extracts and edible fungus pulp to obtain edible / pharmaceutical fungus mixed liquid; (c) culturing inonotus obliquus to obtain inonotus obliquus fermentation broth; (d) individually using saccharomyces, bacillus aceticus , and lactobacillus to ferment fruits and vegetables and the edible / pharmaceutical fungus mixed liquid to obtain fruit and vegetable ferment original liquor and edible / pharmaceutical fungus ferment original liquor; (e) merging the inonotus obliquus fermentation broth, fruit and vegetable ferment original liquor, and edible / pharmaceutical fungus ferment original liquor to obtain the active ferment with a high SOD content. The reutilization of inonotus obliquus fermentation waste liquid is achieved, and the waste liquid can be effectively chelated with ferment to obtain the active ferment with a high SOD content. A long time fermentation, which lasts for about 30 days, is adopted and the active ferment can prominently improve the bioavailability of human body.

The invention discloses a total triterpenoid extract of inonotus obliquus with a hypoglycemic function, which is prepared through the following steps: (1) crushing and sieving inonotus obliquus, adding ethanol aqueous solution for immersion, carrying out ultrasonic extraction, separating the supernatant, and concentrating and drying the supernatant, thereby obtaining ethanol extract; (2) dispersing the ethanol extract into distilled water, oscillating and extracting with petroleum ether, and separating the extract from water-phase residues generated in extraction; and (3) oscillating and extracting the water-phase residues with n-butanol, separating the extract, mixing, concentrating and drying, thereby obtaining the total triterpenoid extract of the inonotus obliquus with the hypoglycemic function, wherein the content of the triterpenoid is 70-300mg / g. the total triterpenoid extract of the inonotus obliquus with the hypoglycemic function provided by the invention can obviously reduce the fasting blood glucose level of mouse with hyperglycemia, can significantly ease the diabetes symptoms of the mouse with hyperglycemia, and has no significant toxicity to major organs.

The invention discloses a preparation method of an inonotus obliquus polysaccharide aqueous solution which is a polysaccharide aqueous solution prepared b performing y microwave digestion, deproteinization and decoloring on crushed inonotus obliquus. The preparation method comprises the following steps: clarifying the polysaccharide aqueous solution with a ZTC1+1III type clarifying agent, wherein the clarification condition is as follows: the dosage of the ZTC1+1III type clarifying agent (1 percent of B component / 1 percent of A component) is between 8 percent / 4 percent and 4 percent / 2 percent, the clarification temperature (B / A) is between 90 DEG C / 60 DEG C and 60 DEG C / 30 DEG C, and the temperature preservation time is between 15 minutes and 35 minutes. The preparation method of the inonotus obliquus polysaccharide aqueous solution has the advantages of simplicity in operation, small loss to inonotus obliquus polysaccharide and high content of effective components, thus being applicable to preparation of health-care products or medicines.

The present invention relates to a compound extract of fomes yucafensis and betulin, its preparation method and preparation. Said preparation method includes the following steps: (1), respectively pulverizing fomes yucafensis and betulin; (2), mixing (by weight portion) 2-8 portions of fomes yucafensis powder and 3.5-11.5 portions of betulin powder; soaking the obtained mixturein water, heating to make water extraction, taking out supernatant fluid to obtain the supernatant fluid A; (3), adding water into the mixture extracted by step 2 and making water extraction, taking out supernatant fluid to obtain supernatant fluid B; (4), mixing the above-mentioned two supernatant fluids to obtain their mixed liquor, removing protein, lipid and impurity; and (5), concentrating and drying said mixed liquor so as to obtain the invented compound extract of fomes yucafensis and belutin.

The invention relates to Inonotus obliquus and a method for extracting triterpennoids from the same. The adopted technical scheme is that the Inonqqus obliquus LNUF 008 has the collection number of CCTCC M 209280. The method for extracting the triterpennoids from the inonotus obliquus comprises the following steps of: activating the inonotus obliquus to prepare seed liquor; inoculating the seed liquor in an inoculation amount which accounts for 2 percent of the volume of fermentation liquor into a fermentation tank liquid synthetic medium, fermenting, performing suction filter on the fermentation liquor, drying at the temperature of 45 DEG C, filtering out mycelia, pouring the mycelia into a mortar, grinding the mycelia into powder, adding an organic solvent in a volume which is 10 times mass of the powder, and immersing for 24 hours; performing ultrasonication at the temperature of 50 DEG C and the frequency of 77 KHz for 60 minutes; and centrifuging for 10 minutes under the condition of 5,000 revolutions / minute, removing sediment, collecting supernatant, and concentrating under reduced pressure to obtain a target product. The triterpennoids extracted from the inonotus obliquus have the advantages of high content and high recovery rate, and provide the feasibility for large-scale production.

The invention discloses a piece of functional bread for treating the cardiovascular disease, and a making method of the functional bread. The bread comprises 70 parts to 100 parts of coarse grain powder, 30 parts to 50 parts of composite functional powder, 6 parts to 8 parts of vegetable oil, 10 parts to 15 parts of egg white, 1.5 parts to 2.5 parts of nutrition additives, 0.002 part to 0.003 part of bread improver, 0.01 part to 0.02 part of corrigent, 0.3 part to 0.5 part of active dry yeast, 0.6 part to 1.0 part of edible salt and 5 parts to 10 parts of acacia honey. The coarse grain powder is the mixture of corn flour, green bean powder, rice bran, wheat bran, adlay powder, whole wheat flour and strong flour. The composite functional powder is the mixture of inonotus obliquus powder, celery seed powder, locust flower powder, kelp powder, hawthorn powder, wild salviae miltiorrhizae powder, yam flour and onion powder. The vegetable oil is the mixture of olive oil, perilla oil and garlic oil. The bread is good in taste, abundant in nutrient and low in fatty acid content, contains a certain number of Chinese medicine components and has a good dietary therapy effect on patients with the cardiovascular disease.

The invention discloses a composition containing inonotus obliquus extract and application of composition, and aims to provide a composition which can form a layer of air breathable film on the surface of skin, effectively reducing the pollutant adhering, enhancing the natural barrier function of skin, effectively resisting irritation, allergy and other problems of outside pollutants to skin; the composition is mainly prepared from, by weight, 10-25 parts of inonotus obliquus extract, 8-20 parts of moringa pterygosperma seed extract, 5-15 parts of Bordeaux leaf extract, 3-10 parts of undaria pinnatifida extract. The composition belongs to the field of cosmetics.

The invention discloses large seaweed extract sunscreen skin care emulsion and a preparation method thereof. The large seaweed extract sunscreen skin care emulsion is prepared by mixing the following components in parts by weight: 30-35 parts of decolorized brown algae extract solution, 30-35 parts of decolorized red alga extract solution, 3-5 parts of nanoscale barium sulfate powder, 3-5 parts of nanoscale silicon dioxide powder, 3-5 parts of glycerin monostearate, 5-7 parts of white oil, 7-8 parts of Vaseline, 2-3 parts of beewax, 1-3 parts of chitosan, 1-3 parts of polysaccharide of inonotus obliquus, 0.3-0.6 part of royal jelly, 1-2 parts of olive oil, 0.5-0.6 part of lauryl sodium sulfate, 0.5-0.6 part of triethanolamine and 15-25 parts of deionized water. The large seaweed extract sunscreen skin care emulsion has high stability and safety, is good in sunscreen property and has the effects of whitening, efficiently supplementing water, nourishing and moisturizing, balancing oil and resisting skin aging.

The invention discloses application of Inonotus obliquus polysaccharide components in preventing and controlling chronic pancreatitis and belongs to the technical field of medicaments. The Inonotus obliquus polysaccharide components are applicable to preventing and controlling chronic pancreatitis. The Inonotus obliquus polysaccharide components which are 0.4g / kg of mouse weight are effective in increasing the content of superoxide dismutase in pancreatic glands of mice with chronic pancreatitis and decreasing accumulation of malondialdehyde and meanwhile are capable of reducing IL-1Beta and IFN-Nu in serum, and effective in decreasing the contents of amylase and lactic dehydrogenase and decreasing synthetic hydroxyproline. An application method of Inonotus obliquus polysaccharide components alleviate the condition of chronic pancreatitis in terms of immunity and metabolic regulation; compared with methods such as accumulated heat and dyspepsia conditioning in traditional Chinese medicine and medicated analgesia and surgery in western medicine, the method has the advantages that no toxic or side effect occurs, surgical pain is avoided, a treatment course is short, treatment is safe and convenient, and the effect of preventing and treating chronic pancreatitis in mice is significant.

The invention discloses a method for extracting triterpene substances from inonotus obliquus by use of quorum sensing molecules induction, wherein the method comprises the following steps: adding activated inonotus obliquus CFCC 83414 into a liquid medium, and performing fermentation culture for 8-14 days at 24-26 DEG C; adding quorum sensing molecules in the 5th-8th days of the fermentation; after the fermentation, separating thalli and fermentation liquid; performing aftertreatment to separate and purify triterpene substances from the thalli and fermentation liquid. According to the method disclosed by the invention, quorum sensing molecules are added in the fermentation metaphase to stimulate growth of inonotus obliquus; the method does not need introduction of a gene engineering strainor additional enzyme, is simple in aftertreatment, and can effectively increase the yield of triterpene substances in inonotus obliquus.

The invention relates to an extraction method of inonotus obliquus polysaccharide. A high-voltage pulse electric field-assisted composite enzyme extraction method is mainly adopted for extracting inonotus obliquus powder multiple times, and the influence of the high-temperature environment of a traditional extraction method on the biological activity of the inonotus obliquus polysaccharide is overcome, and the extraction rate of the inonotus obliquus polysaccharide is improved.

The invention discloses lucid ganoderma tea, lucid ganoderma teabag and a processing technology thereof. The lucid ganoderma tea comprises the following four components in parts by weight: 50-60 parts of inonotus obliquus powder, 10-20 parts of phellinus igniarius powder, 10-20 parts of phellinus linteus powder and 5-30 parts of lucid ganoderma extract. Thus, the lucid ganoderma tea disclosed by the embodiment of the invention has perfect mouthfeel, can give full play to the medicinal effect and healthcare effect of lucid ganoderma, improves the body immunity, and realizes an effect of adjusting and protecting the central nervous system, digestive system, endocrine system and respiratory system and the organs such as liver, prostate and the like.

The invention relates to the technical field of medicament, in particular to inonotus obliquus extracellular and intracellular mixing crude polysaccharide with a function of strengthening immunity, which is extracted from a submerged fermentation product of medical fungus inonotus obliquus liquid. The method comprises the following steps: taking slant strain under an asepsis condition, and inoculating the slant strain into a plate filled with fresh comprehensive solid culture medium; pumping and filtering a secondary fermentation product through primary fermentation and secondary fermentation to obtain inonotus obliquus mycelium and fermentation supernatant; and preparing the inonotus obliquus extracellular and intracellular mixing crude polysaccharide through concentration and water extraction and alcohol precipitation. In vivo experiments prove that the inonotus obliquus extracellular and intracellular mixing crude polysaccharide can prolong the surviving time of mice hepatocellular carcinoma H22 and S180 ascites tumor mice, and can obviously improve the thymus gland index and spleen index of the mice. Furthermore, the inonotus obliquus extracellular and intracellular mixing crude polysaccharide can obviously improve secretion of IL-2, TNF-alpha cell factors in the blood serum of the tumor-bearing mice, and can be used for preparing new immunity intensifying preparation. The invention opens up a new way for development and utilization of medical fungus resources.

The invention provides inonotus obliquus health tea and a production method thereof. The inonotus obliquus health tea provided by the invention is prepared from the following raw materials in parts by weight: 4-8 parts of inonotus obliquus and 4-8 parts of tea leaves. In a production process, the inonotus obliquus is washed, dried and crushed, and then is mixed with the tea leaves to prepare the inonotus obliquus health tea. In the inonotus obliquus health tea, the added tea leaves give a rich aroma to the health tea, and enrich and improve the drinking taste of the inonotus obliquus health tea; and on the aspect of effects, the inonotus obliquus and the tea leaves are scientifically matched to have the synergistic interaction effect, so that the health effects of the tea leaves and the inonotus obliquus are obtained, and the inonotus obliquus health tea also has the remarkable effects of lowering blood lipid, lowering blood pressure and improving the immunity of the organism.

The invention relates to an instant molecular health-care tea, particularly an molecular health-care tea which is prepared from Inonotus obliquus, radix astragali, wolfberry fruit, kudzu root, rhizoma polygonati, radix rehmanniae, radix psuedostellariae, cornel and cinnamon. The health-care traditional Chinese medicines are prepared into the instant tea according to the formula, thereby enhancing the absorption availability; and thus, while people drink tea and chat, the instant tea can control the blood sugar, relieve diabetes, enhance the sensitivity of the insulin receptor and improve the immunity, thereby creating a novel quick method for health care by drinking tea.

The invention belongs to the technical field of microorganisms, and relates to a method for producing inonotus obliquus exopolysaccharides through submerged fermentation, particularly to a method for industrial-scale production of the inonotus obliquus exopolysaccharides. The method comprises the steps as follows: activated inonotus obliquus strains are inoculated into a seed tank for propagation, then propagated liquid seeds are inoculated into a specially-made polysaccharide-producing liquid culture medium for fermentation, and a fermentation liquid is separated to extract the exopolysaccharides. According to the method, research is conducted to overcome defects in the prior art, on the premises that a fermentation culture medium (especially a carbon source) is continuously optimized through small and medium tests and the biomass and the exopolysaccharides are increased, the problems of fermentation process parameter control, extracting equipment model selection, optimization and the like in the process of enlargement from the small tests to industrial production are solved, the strains are successfully inoculated into fermentation tanks of 10-20 t finally for industrial production, the high-quality inonotus obliquus polysaccharides are produced smoothly, and the continuous, automatic and industrial production is actually realized.

The invention relates to a makeup composition, an application thereof and a makeup essence thereof. The makeup composition is prepared from the following active components in parts by weight: 10-60 parts of bio-saccharide gum-4, 1-20 parts of inonotus obliquus plant peptide and 1-10 parts of oat polypeptide, wherein the bio-saccharide gum-4 forms a physical barrier membrane on the surface of skin to prevent dust or fine particle adhesion, so that generation of free radicals on the surface of the skin can be reduced; the inonotus obliquus plant peptide is beneficial to the control of excessive secretion of skin grease and adjustment of the balance of skin water-oil secretion, so that the adhesion of fine particles to the skin is further reduced, and the generation of the free radicals on the skin can be reduced; the oat polypeptide can eliminate free radicals on the skin generated by dust, fine particles and UV (Ultraviolet) radiation and avoid collagen in the skin from being decomposed and natural anti-oxidant from being consumed. The active components have synergistic protection actions on the skin, and play roles in resisting exogenous environmental harm through multiple protections on the skin.

The invention discloses a preparation and conversion method of inonotus obliquus protoplast. The preparation method comprises the following steps that a mixed enzyme solution composed of lywallzyme and driselase is adopted for enzymolysis of an inonotus obliquus hypha membrane and filtered through eight layers of lens wiping paper, filtrate collection and centrifugation are carried out, and sediment is obtained and is the inonotus obliquus protoplast. The conversion method comprises the steps that plasmid pAN7-1 is induced by PEG / CaC12 to be converted into inonotus obliquus protoplast, and inonotus obliquus containing plasmid pAN7-1 is obtained. According to the inonotus obliquus protoplast obtained through the method, the yield reaches up to 5*107 / mL, and the regeneration rate is 7%. The preparation and conversion method lays a foundation for carrying out genetic manipulation modification on the inonotus obliquus in the later period, improving the yield of a triterpene compound and a precursor thereof, then improving the medical value of the inonotus obliquus and meeting market requirements.

The invention provides an inonotus obliquus tea bag. The inonotus obliquus tea bag is characterized by being prepared from inonotus obliquus as a main material and proper amount of added tea, astragalus membranaceus and medlar. The tea bag not only is convenient to drink, clean and sanitary, but also contains a plurality of nutritional ingredients, accords with modern fast-paced life characteristics of people, and has the effects of adjusting sugar content of blood, preventing cardiovascular and cerebrovascular diseases and improving the immunity of the organism after being drunk for a long period of time.

The invention discloses immature tangerine tea capable of preserving health and strengthening the stomach and a preparation method thereof. The immature tangerine tea capable of preserving health andstrengthening the stomach is prepared from the following raw materials in parts by weight: 10-25 parts of immature tangerine, 10-15 parts of black tartary buckwheat tea, 8-15 parts of black tea, 10-15parts of inonotus obliquus and 3-5 parts of clove flower tea. The preparation method of the immature tangerine tea capable of preserving health and strengthening the stomach comprises the following steps: cutting an opening in the top of the immature tangerine after pre-treatment, and forming vent holes in a bottom surface and side surfaces of the immature tangerine to obtain a tangerine fruit husk; mixing the black tea, the clove flower tea, the inonotus obliquus and the black tartary buckwheat tea to obtain a tea mixture; and putting the tea mixture into the tangerine fruit husk through theopening of the tangerine fruit husk, sealing the opening of the tangerine fruit husk with a cover, and then, performing baking and airing to obtain a finished product. The immature tangerine tea capable of preserving health and strengthening the stomach, disclosed by the invention, is simple in preparation technology and convenient to carry; and by virtue of synergistic action of all components,the immature tangerine tea can achieve the effects of improving gastritis, tranquilizing and allaying excitement and enhancing immunity after being drunk for a long term.

The invention discloses a method of extracting and preparing polysaccharide from inonotus obliquus. The method is characterized by comprising the following steps: A, degreasing pre-treatment of raw materials; B, preparation of coarse polysaccharide by hot water extraction; C, enzymolysis of coarse polysaccharide; D, treatment by macroporous resin DM301; E, ultrafiltration for removing small molecules; and F, vacuum freeze-drying. The decolored refined polysaccharide of inonotus obliquus with most proteins and small molecular impurities removed is finally prepared by using the process; and the method is suitable for mass industrial production.

The invention discloses an inonotus obliquus submerged fermentation product and application. The inonotus obliquus submerged fermentation product comprises a composition A, a composition B and a composition C. By the utilization of a biological fermentation technology, inonotus obliquus strains are fermented to obtain the new composition, and the composition belongs to a natural alpha-glucosidase inhibitor, and is low in price and environmentally friendly; the preparation technology is easy to industrialize. The inonotus obliquus submerged fermentation product is suitable for a broad crowd of people, convenient to eat and capable of serving as a daily drink to be drunk by people with diabetes, will not generate adverse reactions such as abdominal discomfort, flatulence and exsufflation, and can achieve a health-care effect after being drunk by average persons.

The invention discloses a method for preparing inonotus obliquus (Fr.)Pilat crude polysaccharides by utilizing resin AB-8 with weak polarity. The method comprises the following steps: A. carrying out degreasing pretreatment on raw materials; B. carrying out hot water extraction to prepare crude polysaccharides; C. carrying out enzymolysis on the crude polysaccharides; D. carrying out treatment with the macroporous adsorption resin AB-8; E. carrying out ultrafiltration to remove small molecules; F. carrying out vacuum freeze drying, thus preparing the inonotus obliquus (Fr.)Pilat polysaccharides. The discolored refined inonotus obliquus (Fr.)Pilat polysaccharides from which most protein and small molecule impurities are removed, which are finally obtained by using the process, have higher polysaccharide retention rates and high decolorization rates and protein removal rates. The method is suitable for industrially producing the refined inonotus obliquus (Fr.)Pilat polysaccharides in a large scale.

The invention relates to fermented birch juice and a production method thereof. The fermented birch juice is obtained through fermentation by taking inonotus obliquus as a strain and birch juice as asubstrate. The method comprises the steps of performing fermentation by taking the inonotus obliquus as a strain and the birch juice as a substrate.

A method for extracting water soluble beta-glucan from inonotus obliquus sporophore comprises the following steps: 1, inonotus obliquus crude polysaccharide preparation, to be more specific, taking inonotus obliquus sporophore, crushing to obtain powder, extracting the powder with lye, filtering to remove a residue, adding ethanol into filtrate, standing, filtering to obtain a precipitate to obtain inonotus obliquus crude polysaccharide; 2, inonotus obliquus beta-glucan preparation, to be more specific, taking the inonotus obliquus crude polysaccharide, drying, adding water, mixing, adding alpha-amylase for hydrolysis, filtering hydrolysate, removing filtrate to obtain a residue to obtain inonotus obliquus beta-glucan; and 3, water soluble beta-glucan preparation, to be more specific, taking the inonotus obliquus beta-glucan, adding lye for stirring and extraction, adjusting pH of the extracting solution to 2.0-6.8, heating for hydrolysis, filtering hydrolysate, adding ethanol into filtrate, standing, separating a precipitate and drying to obtain the soluble beta-glucan. According to the method, the yield of inonotus obliquus polysaccharide is improved, water solubility is increased, and the water soluble beta-glucan is more conducive to body absorption.

The invention relates to an inonotus obliquus deep culture method, comprising the following steps: 1. slant culture; 2. primary, secondary and tertiary seed culture; 3. deep fermentation culture; wherein, the deep fermentation culture has the following conditions: (1) fermentation culture medium consists of 0.1-10% of corn saccharification liquid, 0.1-10% of soluble starch, 0.1-5% of yeast extract powder, 0.1-5% of peptone, 0.01-1% of KH2PO4, and 0.01-1% of MgSO4; (2) fermentation cycle is 3-15d; (3) culture temperature is 20-35 DEG C; (4) initial pH value of culture medium is 4-8; (5) rotation speed of table concentrator is 50-200r / min; (6) inoculation quantity is 5-20%; and (7) liquid volume is 1 / 10-4 / 5. The invention uses fermentation engineering technology to produce inonotus obliquus, obtains quantities of reproduced strains through the primary, secondary and tertiary seed culture, makes up the defect of insufficient productivity natural bacteria, has obvious economic significance, effectively avoids contamination of microorganism as the fermentation is carried out under the aseptic condition, and provides beneficial condition for preparing the inonotus obliquus.