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Method for preparing inonotus obliquus (Fr.)Pilat crude polysaccharides by utilizing resin AB-8 with weak polarity

A technology of AB-8, Inonotus obliquus, applied in the field of biology

Active Publication Date: 2014-09-17
哈尔滨神桦汇康生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Macroporous resin used to remove crude polysaccharides from Inonotus obliquus has not been reported in the literature

Method used

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  • Method for preparing inonotus obliquus (Fr.)Pilat crude polysaccharides by utilizing resin AB-8 with weak polarity
  • Method for preparing inonotus obliquus (Fr.)Pilat crude polysaccharides by utilizing resin AB-8 with weak polarity
  • Method for preparing inonotus obliquus (Fr.)Pilat crude polysaccharides by utilizing resin AB-8 with weak polarity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 The method for preparing the crude polysaccharide of Inonotus obliquus by utilizing AB-8 weak polarity resin comprises the steps:

[0019] A. Degreasing pretreatment of raw materials: take the dried Inonotus obliquus sclerotia, crush them, soak them in 4 times the volume of 60% ethanol solution for 16 hours to remove fat, some mono (oligo) sugars and fat-soluble pigments, and keep the residue in a cool place Let dry.

[0020] B. Preparation of crude polysaccharide by hot water extraction: After the pretreated residue is air-dried, add 10 times distilled water (v / w), extract in boiling water for 1 h, filter with 80-mesh gauze, concentrate the filtrate, centrifuge, and spray-dry to obtain crude polysaccharide.

[0021] C. Crude polysaccharide enzymatic hydrolysis: dissolve the crude polysaccharide in water, prepare a 0.5% (w / w) aqueous solution, add papain, the amount of papain added is 3% (w / w) of the crude polysaccharide aqueous solution, and react at 35°...

Embodiment 2

[0025] Example 2 The method for preparing the crude polysaccharide of Inonotus obliquus by utilizing AB-8 weak polarity resin comprises the steps:

[0026] A. Degreasing pretreatment of raw materials: Take the dried Inonotus obliquus sclerotia, crush them, soak them in 8 times the volume of 95% ethanol solution for 24 hours to remove fat, some mono (oligo) sugars and fat-soluble pigments, and keep the residue in a cool place Let dry.

[0027] B. Preparation of crude polysaccharide by hot water extraction: After the pretreated residue is air-dried, add 20 times distilled water (v / w), extract with boiling water for 3 hours, filter with 120-mesh gauze, repeat the extraction of the filter residue twice, combine the filtrate, concentrate, centrifuge, and spray Dried crude polysaccharides.

[0028] C. Crude polysaccharide enzymatic hydrolysis: dissolve the crude polysaccharide in water to prepare a 2.0% (w / w) aqueous solution, add papain, the amount of papain added is 5% (w / w)...

Embodiment 3

[0033] Example 3 Utilize AB-8 weak polarity resin to prepare the method for crude polysaccharide of Inonotus obliquus, it comprises the steps:

[0034] A. Degreasing pretreatment of raw materials: take the dried Inonotus obliquus sclerotia, crush them, soak them in 7 times the volume of 90% ethanol solution for 20 hours to remove fat, some mono (oligo) sugars and fat-soluble pigments, and keep the residue in a cool place Let dry.

[0035]B. Preparation of crude polysaccharide by hot water leaching: After the pretreated residue is air-dried, add 18 times distilled water (v / w), extract with boiling water for 2.5 hours, filter with 100-mesh gauze, repeat the extraction of the filter residue 3 times, combine the filtrate, concentrate, centrifuge, and spray Dried crude polysaccharides.

[0036] C. Crude polysaccharide enzymatic hydrolysis: dissolve the crude polysaccharide in water to prepare a 1.8% (w / w) aqueous solution, add papain, the amount of papain added is 4.5% (w / w) ...

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Abstract

The invention discloses a method for preparing inonotus obliquus (Fr.)Pilat crude polysaccharides by utilizing resin AB-8 with weak polarity. The method comprises the following steps: A. carrying out degreasing pretreatment on raw materials; B. carrying out hot water extraction to prepare crude polysaccharides; C. carrying out enzymolysis on the crude polysaccharides; D. carrying out treatment with the macroporous adsorption resin AB-8; E. carrying out ultrafiltration to remove small molecules; F. carrying out vacuum freeze drying, thus preparing the inonotus obliquus (Fr.)Pilat polysaccharides. The discolored refined inonotus obliquus (Fr.)Pilat polysaccharides from which most protein and small molecule impurities are removed, which are finally obtained by using the process, have higher polysaccharide retention rates and high decolorization rates and protein removal rates. The method is suitable for industrially producing the refined inonotus obliquus (Fr.)Pilat polysaccharides in a large scale.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing crude polysaccharides from Inonotus obliquus by using AB-8 weak polarity resin. Background technique [0002] Inonotus obliquus[ Inonotus obliquus (Fr.) Pilat] is a famous folk wild medicinal fungus. Studies have shown that Inonotus obliquus polysaccharide is one of the important active ingredients, which has various pharmacological activities such as enhancing immune regulation, anti-tumor, anti-oxidation, lowering blood sugar and anti-virus, and has become one of the hot research fields at home and abroad. However, there are relatively few reports on the isolation and purification of polysaccharides from Inonotus obliquus. [0003] Due to the complexity of the composition of polysaccharides, the molecular weight distribution of the crude polysaccharide extract is wide, and there are many types of polysaccharides, and previous experiments have s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 陈文灵
Owner 哈尔滨神桦汇康生物有限公司
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