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Method for extracting water soluble beta-glucan from inonotus obliquus sporophore

The technology of Inonotus chinensis and Inonotus obliquus is applied in the field of extraction of active components of traditional Chinese medicine, and can solve the problems of poor water solubility, difficult extraction, poor polysaccharide absorption, etc., and achieves improved yield, increased water solubility, and is beneficial to human body absorption. Effect

Inactive Publication Date: 2016-03-09
HEILONGJIANG JOHNSUN BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The large molecular weight part of the polysaccharide of Inonotus obliquus is poor in water solubility, and it is difficult to extract completely with water as a solvent in the separation and extraction. Even if the lye is used for extraction, although the extraction rate is high, the polysaccharide product obtained after drying is still insoluble in water.
The human body does not absorb this macromolecule Inonotus obliquus polysaccharide well

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Weigh 1 kg of Inonotus obliquus fruiting body and pulverize it, pass through a 50-mesh sieve, add 5 liters of 0.1 mol / liter sodium hydroxide solution to the powder and extract for 1 hour, filter the residue with a plate basket of 0.5 micron pore filter cloth, and obtain 4.6 liters of filtrate . Add 18.4 liters of absolute ethanol to the filtrate, let it stand for 2 hours, and filter it with a plate basket with a pore size of 0.5 micron to obtain a precipitate, which weighs a total of 150 grams after drying. Add 750 ml of water to the precipitate and stir at room temperature for 10 minutes, add 1 gram of fungal α-amylase solution (Shandong Jienuo Bioenzyme Co., Ltd.), adjust the pH value to 5.5 with 6 mol / L hydrochloric acid, and incubate at 50 degrees Celsius for 24 hours. Then filter with a plate basket with 0.5 micron aperture cloth, discard the filtrate, add 0.8 liter of 1.0 mol / L sodium hydroxide to the residue and stir for extraction for 2 hours, adjust the acidi...

Embodiment 2

[0026] Weigh 1 kg of Inonotus obliquus fruiting bodies and pulverize them, pass through a 50-mesh sieve, add 10 liters of 1 mol / liter sodium hydroxide solution to the powder and extract for 5 hours, filter through a plate basket with a filter cloth of 0.5 microns in diameter to obtain 7.2 liters of filtrate . Add 28.8 liters of absolute ethanol to the filtrate, let it stand for 2 hours, and filter it with a plate basket with a pore size of 0.5 micron to obtain a precipitate, which weighs a total of 142 grams after drying. Add 1.4 liters of water to the precipitate and stir at room temperature for 10 minutes, add 0.6 g of fungal α-amylase solution (purchased from Wuxi Chengxing Biotechnology Co., Ltd.), adjust the pH value to 5.8 with 2 mol / L hydrochloric acid, and incubate at 50 degrees Celsius for 24 hours. Then filter with a plate basket with 0.5 micron aperture cloth, discard the filtrate, add 0.8 liter of 1.0 mol / liter of sodium hydroxide to the residue and stir for extr...

Embodiment 3

[0028] Weigh 1 kg of Inonotus obliquus fruiting body and pulverize it, pass through a 50-mesh sieve, add 20 liters of sodium hydroxide solution of 0.5 mol / liter to the powder and extract for 10 hours, filter the residue with a plate basket of filter cloth with 2 micron apertures, and obtain 16.8 liters of filtrate . Add 67.2 liters of absolute ethanol to the filtrate, let it stand for 2 hours, and filter it with a plate basket with a cloth of 2 microns to obtain a precipitate, which weighs a total of 143 grams after drying. Add 1 liter of water to the precipitate and stir at room temperature for 10 minutes, add 1 gram of fungal α-amylase solution (Shandong Jienuo Bioenzyme Co., Ltd.), and keep the pH value at 5.5, 50 degrees Celsius for 24 hours. Then filter with a plate basket with 4 micron pore size cloth, discard the filtrate, add 0.6 mol / liter of sodium hydroxide and 0.8 liters of sodium hydroxide to stir and extract for 2 hours, adjust the acidity and alkalinity of the ...

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PUM

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Abstract

A method for extracting water soluble beta-glucan from inonotus obliquus sporophore comprises the following steps: 1, inonotus obliquus crude polysaccharide preparation, to be more specific, taking inonotus obliquus sporophore, crushing to obtain powder, extracting the powder with lye, filtering to remove a residue, adding ethanol into filtrate, standing, filtering to obtain a precipitate to obtain inonotus obliquus crude polysaccharide; 2, inonotus obliquus beta-glucan preparation, to be more specific, taking the inonotus obliquus crude polysaccharide, drying, adding water, mixing, adding alpha-amylase for hydrolysis, filtering hydrolysate, removing filtrate to obtain a residue to obtain inonotus obliquus beta-glucan; and 3, water soluble beta-glucan preparation, to be more specific, taking the inonotus obliquus beta-glucan, adding lye for stirring and extraction, adjusting pH of the extracting solution to 2.0-6.8, heating for hydrolysis, filtering hydrolysate, adding ethanol into filtrate, standing, separating a precipitate and drying to obtain the soluble beta-glucan. According to the method, the yield of inonotus obliquus polysaccharide is improved, water solubility is increased, and the water soluble beta-glucan is more conducive to body absorption.

Description

technical field [0001] The invention relates to the field of extraction of active ingredients of traditional Chinese medicines, in particular to a method for extracting water-soluble β-glucan from fruit bodies of Inonotus obliquus. Background technique [0002] Inonotus obliquus is a rare fungus with high medicinal value, and its active ingredients are widely used in food, medicine and health care products. With the in-depth research on Inonotus obliquus, a large number of active ingredients have been isolated, mainly including: Inonotus obliquus polysaccharide, obliquulin, obliquulin alcohol and various oxidized triterpenoids. Inonotus obliquus has the functions of inhibiting tumor growth, enhancing immune function, anti-oxidation and anti-virus. [0003] With the increasing application of Inonotus obliquus, the extraction of active ingredients from Inonotus obliquus has become a hot topic. Inonotus obliquus polysaccharide is one of the main active components of Inonotus ...

Claims

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Application Information

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IPC IPC(8): C08B37/02
CPCC08B37/0024
Inventor 陈喜军
Owner HEILONGJIANG JOHNSUN BIOLOGICAL ENG CO LTD
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