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Preparation method of inonotus obliquus polysaccharide aqueous solution

A technology of Inonotus obliquus and its aqueous solution, which is applied in food preparation, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve problems affecting product quality and commodity value, precipitation, turbidity, etc., and achieve low cost and simple operation , the effect of small loss

Active Publication Date: 2012-07-25
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Inonotus obliquus polysaccharide, as an extremely important pharmaceutical ingredient, has broad development prospects, but the aqueous solution of Inonotus obliquus polysaccharides produces precipitation and turbidity during storage, which affects the quality and commodity value of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the preparation of ZTC1+1III type natural clarifier solution

[0024] Preparation of component A solution: Weigh 5.0g of component A of ZTC1+1III type natural clarifier (Tianjin Zhentiancheng Technology Co., Ltd.), first stir it into a paste with a small amount of distilled water, then add distilled water to 500mL, swell for 24 hours, and stir well , double-layer gauze filter, that is, 1% A component viscose solution.

[0025] Preparation of component B solution: Weigh 5.0g of component B of ZTC1+1III clarifier, dissolve it with a small amount of 1% acetic acid solution and stir it into a paste, then add 1% acetic acid to 500mL, swell for 24 hours, stir well, double layer Filter through gauze to obtain a viscous solution of 1% component B.

Embodiment 2

[0027] Add the crushed and sieved Inonotus obliquus to 50 times the volume of water, place it in a microwave extraction equipment (Qingdao Aucma, AM-C23), microwave power 600W, wavelength 1mm-1m, microwave extraction for 20min, and concentrate the extract volume in vacuum to the original 1 / 10, add absolute ethanol to a final ethanol concentration of 80%, let it stand overnight, centrifuge to collect the precipitate, dry and weigh, the obtained Inonotus obliquus extract is the crude polysaccharide of Inonotus obliquus, and the yield is 18%, which contains protein, pigment, polysaccharide of Inonotus obliquus, etc.

[0028] The extracted Inonotus obliquus extract is deproteinized by trichloroacetic acid (TCA) method, the amount of TCA is 20% (volume ratio), the temperature is 60°C, and the heating time is 20min. The resulting solution is analyzed, and the protein removal rate is 50%, and the polysaccharide loss rate of 19.8%.

[0029] The deproteinized Inonotus obliquus extract...

Embodiment 3

[0032] Add the crushed and sieved Inonotus obliquus to 70 times the volume of water, place it in a microwave extraction equipment (Qingdao Aucma, AM-C23), microwave power 800W, wavelength 1mm ~ 1m, microwave extraction for 25min, and concentrate the extract volume in vacuum to the original 1 / 10, add absolute ethanol to the final ethanol concentration of 80%, let it stand overnight, centrifuge to collect the precipitate, dry and weigh, the obtained Inonotus obliquus extract is the crude polysaccharide of Inonotus obliquus, the crude polysaccharide The yield was 24%.

[0033] The extracted Inonotus obliquus extract was deproteinized by trichloroacetic acid (TCA) method, the amount of TCA was 50%, the temperature was 80° C., and the heating time was 50 minutes. The protein removal rate was 48%, and the polysaccharide loss rate was 21%.

[0034] The deproteinized Inonotus obliquus extract was diluted 60 times with water and decolorized with activated carbon, the dosage of activate...

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Abstract

The invention discloses a preparation method of an inonotus obliquus polysaccharide aqueous solution which is a polysaccharide aqueous solution prepared b performing y microwave digestion, deproteinization and decoloring on crushed inonotus obliquus. The preparation method comprises the following steps: clarifying the polysaccharide aqueous solution with a ZTC1+1III type clarifying agent, wherein the clarification condition is as follows: the dosage of the ZTC1+1III type clarifying agent (1 percent of B component / 1 percent of A component) is between 8 percent / 4 percent and 4 percent / 2 percent, the clarification temperature (B / A) is between 90 DEG C / 60 DEG C and 60 DEG C / 30 DEG C, and the temperature preservation time is between 15 minutes and 35 minutes. The preparation method of the inonotus obliquus polysaccharide aqueous solution has the advantages of simplicity in operation, small loss to inonotus obliquus polysaccharide and high content of effective components, thus being applicable to preparation of health-care products or medicines.

Description

technical field [0001] The invention relates to a method for extracting the polysaccharide of the medicinal fungus Inonotus obliquus and preparing an aqueous solution of the polysaccharide. Background technique [0002] Inonotus obliquus (Fr.) Pilat is a rare and valuable medicinal fungus. In taxonomy, Inonotus obliquus belongs to Basidiomycotina-Hymenomycetes-Aphyllophorales-Polyporaceae-Poriahy pobrunnea Peteh ). The scientific name of Inonotus obliquus is Phaeoporus obliquus (pers: Fr.) J.Schroet. In many foreign language materials, Inonotus obliquus is written as Fuscoporia oblique (Pers. Fr) Aoshi or Inontus obliquus (Fr.) Pilat. Among its common names, the Chinese names are chaga, black chaga, chaga, etc., and the foreign names are blaekbirehtuehwood, Malalonmushroom, カバノアナナタケ, etc., and the Russian name is Chaga. Inonotus obliquus is extremely cold-resistant, and the mycelium living in wood such as birch can withstand a low temperature of -40°C. Inonotus obliquus ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C08B37/02A23L1/09A61K31/716A61P3/06
Inventor 李荣杰
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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