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Active ferment with high SOD content and preparation method thereof

A technology of active enzymes and fruit and vegetable enzymes, applied in the field of fermentation engineering, can solve problems such as difficult control of product quality, complicated process technology, and easy contamination by bacteria, and achieve the effects of removing intestinal garbage, inhibiting reproduction, and enhancing immunity

Inactive Publication Date: 2016-01-13
江苏阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzymes usually use fruits, vegetables and edible fungi as raw materials. By adding different probiotics, the process can range from a few months to a few years. The process is complex and takes a long time. It is easy to be contaminated by bacteria and the product quality is difficult to control.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Seed culture of Inonotus obliquus

[0042] The formula of Inonotus obliquus seed medium is: glucose 2%, soybean cake powder 1%, beef extract 0.3%, peptone 0.05%, KH 2 PO 4 0.05%, MgSO 4 0.02%, pH 6.0; the culture medium was sterilized by damp heat at 121°C for 20 minutes, cooled to room temperature, inoculated with 1-2 rings of Inonotus obliquus slant seeds, cultivated for 7 days at 28°C, with a rotation speed of 160rpm, to obtain shake flask seeds.

[0043] 2. Seed culture of yeast

[0044] The formula of the yeast seed medium is: 2% glucose, 0.5% yeast extract, 0.5% peptone, 0.05% NaCl, pH5-7, the medium is sterilized by damp heat at 121°C for 20 minutes, and after cooling, inoculate 1-2 rings of yeast test tube strains , 28-30° C., on a shaker at 150 rpm, and cultured for 3 days to obtain shaker flask seeds.

[0045] 3. Seed culture of Acetobacter

[0046] The formula of the Acetobacter seed medium is: 3% glucose, 3% yeast powder, PH5.5, 4% alcohol (food grad...

Embodiment 2

[0055] 1. Seed culture of Inonotus obliquus

[0056] The formula of Inonotus obliquus seed medium is: glucose 5%, soybean cake powder 2%, beef extract 0.1%, peptone 0.01%, KH 2 PO 4 0.01%, MgSO 4 0.01%, pH 6.0; the culture medium was sterilized by damp heat at 121°C for 20 minutes, cooled to normal temperature, inoculated with 1-2 rings of Inonotus obliquus slant seeds, cultured at 30°C, rotating speed 160rpm, for 6 days to obtain shake flask seeds.

[0057] 2. Seed culture of yeast

[0058] The formula of the yeast seed medium is: 2% glucose, 0.5% yeast extract, 0.5% peptone, 0.05% NaCl, pH5-7, the medium is sterilized by damp heat at 121°C for 20 minutes, and after cooling, inoculate 1-2 rings of yeast test tube strains , 28-30° C., on a shaker at 150 rpm, and cultured for 3 days to obtain shaker flask seeds.

[0059] 3. Seed culture of Acetobacter

[0060] The formula of the Acetobacter seed medium is: 2% glucose, 5% yeast powder, PH5.5, 4% alcohol (food grade) is adde...

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PUM

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Abstract

The invention discloses an active ferment with a high SOD content and a preparation method thereof. The preparation method comprises the following steps: (a) cutting different fruits and vegetables into slices, grinding the slices, and adding white sugar; (b) pulping edible fungus, extracting cordyceps militaris sporocarp and hericium erinaceus sporocarp to obtain extracts, and mixing extracts and edible fungus pulp to obtain edible / pharmaceutical fungus mixed liquid; (c) culturing inonotus obliquus to obtain inonotus obliquus fermentation broth; (d) individually using saccharomyces, bacillus aceticus , and lactobacillus to ferment fruits and vegetables and the edible / pharmaceutical fungus mixed liquid to obtain fruit and vegetable ferment original liquor and edible / pharmaceutical fungus ferment original liquor; (e) merging the inonotus obliquus fermentation broth, fruit and vegetable ferment original liquor, and edible / pharmaceutical fungus ferment original liquor to obtain the active ferment with a high SOD content. The reutilization of inonotus obliquus fermentation waste liquid is achieved, and the waste liquid can be effectively chelated with ferment to obtain the active ferment with a high SOD content. A long time fermentation, which lasts for about 30 days, is adopted and the active ferment can prominently improve the bioavailability of human body.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, in particular to an enzyme with high SOD activity and a preparation method thereof. Background technique [0002] Inonotus obliquus (Inonotus obliquus), is a rare large medicinal fungus, also known as: Chaga, English name: Chaga, etc. or Polyporacea), Polyporaceae, Brown Poreus (Cycloporus genus). Mainly parasitize under the bark of birch, silver birch, elm, alder, etc. or under the bark of vitality wood or on the withered trees after felling. The fungus rarely forms fruiting bodies, and its sterile fruiting bodies are tumor-shaped, hard in appearance, yellowish brown to black, and have obvious uneven and cracked small pieces. First born under the bark, exposed after peeling, flat spread, different sizes, initially white, gradually turning yellow, light brown to brown, basidiospores are colorless to light brown, and the flesh tissue consists of brown bristly hyphae and light yellow Comp...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23L1/03A23L1/30
CPCA23V2002/00A23V2200/30
Inventor 厉文成曾化伟王文风徐国华王英燕张芙蓉
Owner 江苏阜丰生物科技有限公司
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