Active ferment with high SOD content and preparation method thereof
A technology of active enzymes and fruit and vegetable enzymes, applied in the field of fermentation engineering, can solve problems such as difficult control of product quality, complicated process technology, and easy contamination by bacteria, and achieve the effects of removing intestinal garbage, inhibiting reproduction, and enhancing immunity
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Embodiment 1
[0041] 1. Seed culture of Inonotus obliquus
[0042] The formula of Inonotus obliquus seed medium is: glucose 2%, soybean cake powder 1%, beef extract 0.3%, peptone 0.05%, KH 2 PO 4 0.05%, MgSO 4 0.02%, pH 6.0; the culture medium was sterilized by damp heat at 121°C for 20 minutes, cooled to room temperature, inoculated with 1-2 rings of Inonotus obliquus slant seeds, cultivated for 7 days at 28°C, with a rotation speed of 160rpm, to obtain shake flask seeds.
[0043] 2. Seed culture of yeast
[0044] The formula of the yeast seed medium is: 2% glucose, 0.5% yeast extract, 0.5% peptone, 0.05% NaCl, pH5-7, the medium is sterilized by damp heat at 121°C for 20 minutes, and after cooling, inoculate 1-2 rings of yeast test tube strains , 28-30° C., on a shaker at 150 rpm, and cultured for 3 days to obtain shaker flask seeds.
[0045] 3. Seed culture of Acetobacter
[0046] The formula of the Acetobacter seed medium is: 3% glucose, 3% yeast powder, PH5.5, 4% alcohol (food grad...
Embodiment 2
[0055] 1. Seed culture of Inonotus obliquus
[0056] The formula of Inonotus obliquus seed medium is: glucose 5%, soybean cake powder 2%, beef extract 0.1%, peptone 0.01%, KH 2 PO 4 0.01%, MgSO 4 0.01%, pH 6.0; the culture medium was sterilized by damp heat at 121°C for 20 minutes, cooled to normal temperature, inoculated with 1-2 rings of Inonotus obliquus slant seeds, cultured at 30°C, rotating speed 160rpm, for 6 days to obtain shake flask seeds.
[0057] 2. Seed culture of yeast
[0058] The formula of the yeast seed medium is: 2% glucose, 0.5% yeast extract, 0.5% peptone, 0.05% NaCl, pH5-7, the medium is sterilized by damp heat at 121°C for 20 minutes, and after cooling, inoculate 1-2 rings of yeast test tube strains , 28-30° C., on a shaker at 150 rpm, and cultured for 3 days to obtain shaker flask seeds.
[0059] 3. Seed culture of Acetobacter
[0060] The formula of the Acetobacter seed medium is: 2% glucose, 5% yeast powder, PH5.5, 4% alcohol (food grade) is adde...
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