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Method for extracting and preparing polysaccharide from inonotus obliquus

A technology of Inonotus obliquus and polysaccharides, applied in the biological field, to achieve the effect of improving the removal rate, no toxic side effects, and high yield

Inactive Publication Date: 2014-09-17
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Macroporous resin used to remove crude polysaccharides from Inonotus obliquus has not been reported in the literature

Method used

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  • Method for extracting and preparing polysaccharide from inonotus obliquus
  • Method for extracting and preparing polysaccharide from inonotus obliquus
  • Method for extracting and preparing polysaccharide from inonotus obliquus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 The method for extracting and preparing the polysaccharide of Inonotus obliquus from Inonotus obliquus comprises the following steps:

[0020] A. Degreasing pretreatment of raw materials: take the dried Inonotus obliquus sclerotia, crush them, soak them in 4 times the volume of 60% ethanol solution for 16 hours to remove fat, some mono (oligo) sugars and fat-soluble pigments, and keep the residue in a cool place Let dry.

[0021] B. Preparation of crude polysaccharide by hot water extraction: After the pretreated residue is air-dried, add 10 times distilled water (v / w), extract in boiling water for 1 h, filter with 80-mesh gauze, concentrate the filtrate, centrifuge, and spray-dry to obtain crude polysaccharide.

[0022] C. Crude polysaccharide enzymatic hydrolysis: dissolve the crude polysaccharide in water to prepare a 0.5% (w / w) aqueous solution, add papain, the amount of papain added is 3% (w / w) of the crude polysaccharide aqueous solution, react at 3...

Embodiment 2

[0026] Example 2 The method for extracting and preparing the polysaccharide of Inonotus obliquus from Inonotus obliquus comprises the following steps:

[0027] A. Degreasing pretreatment of raw materials: Take the dried Inonotus obliquus sclerotia, crush them, soak them in 8 times the volume of 95% ethanol solution for 24 hours to remove fat, some mono (oligo) sugars and fat-soluble pigments, and keep the residue in a cool place Let dry.

[0028] B. Preparation of crude polysaccharide by hot water extraction: After the pretreated residue is air-dried, add 20 times distilled water (v / w), extract with boiling water for 3 hours, filter with 120-mesh gauze, repeat the extraction of the filter residue twice, combine the filtrate, concentrate, centrifuge, and spray Dried crude polysaccharides.

[0029] C. Crude polysaccharide enzymatic hydrolysis: dissolve the crude polysaccharide in water to prepare a 2.0% (w / w) aqueous solution, add papain, the amount of papain added is 5% (...

Embodiment 3

[0034] Example 3 The method for extracting and preparing the polysaccharide of Inonotus obliquus from Inonotus obliquus, it comprises the steps:

[0035] A. Degreasing pretreatment of raw materials: take the dried Inonotus obliquus sclerotia, crush them, soak them in 7 times the volume of 90% ethanol solution for 20 hours to remove fat, some mono (oligo) sugars and fat-soluble pigments, and keep the residue in a cool place Let dry.

[0036] B. Preparation of crude polysaccharide by hot water leaching: After the pretreated residue is air-dried, add 18 times distilled water (v / w), extract with boiling water for 2.5 hours, filter with 100-mesh gauze, repeat the extraction of the filter residue 3 times, combine the filtrate, concentrate, centrifuge, and spray Dried crude polysaccharides.

[0037] C. Crude polysaccharide enzymatic hydrolysis: dissolve the crude polysaccharide in water to prepare a 1.8% (w / w) aqueous solution, add papain, the amount of papain added is 4.5% (w / ...

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Abstract

The invention discloses a method of extracting and preparing polysaccharide from inonotus obliquus. The method is characterized by comprising the following steps: A, degreasing pre-treatment of raw materials; B, preparation of coarse polysaccharide by hot water extraction; C, enzymolysis of coarse polysaccharide; D, treatment by macroporous resin DM301; E, ultrafiltration for removing small molecules; and F, vacuum freeze-drying. The decolored refined polysaccharide of inonotus obliquus with most proteins and small molecular impurities removed is finally prepared by using the process; and the method is suitable for mass industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting and preparing polysaccharides from Inonotus obliquus. Background technique [0002] Inonotus obliquus[ Inonotus obliquus (Fr.) Pilat] is a famous folk wild medicinal fungus. Studies have shown that Inonotus obliquus polysaccharide is one of the important active ingredients, which has various pharmacological activities such as enhancing immune regulation, anti-tumor, anti-oxidation, lowering blood sugar and anti-virus, and has become one of the hot research fields at home and abroad. However, there are relatively few reports on the isolation and purification of polysaccharides from Inonotus obliquus. [0003] Due to the complexity of the composition of polysaccharides, the molecular weight distribution of the crude polysaccharide extract is wide, and there are many types of polysaccharides, and previous experiments have shown that the extracted c...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 杜秀菊张扬刘珊
Owner LIAOCHENG UNIV
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