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Submerged fermentation technology of producing immunological enhancement active materials of inonotus obliquus

A technology of liquid submerged fermentation and Inonotus obliquus is applied in the field of fermentation engineering to achieve the effects of simple processing, low production cost and good activity

Inactive Publication Date: 2013-06-19
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, there is no effective method for artificially cultivating sclerotia and fruiting bodies at present, so it is a feasible alternative to cultivate mycelia to obtain active ingredients by liquid submerged fermentation.

Method used

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  • Submerged fermentation technology of producing immunological enhancement active materials of inonotus obliquus
  • Submerged fermentation technology of producing immunological enhancement active materials of inonotus obliquus
  • Submerged fermentation technology of producing immunological enhancement active materials of inonotus obliquus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Strain activation: Inonotus obliquus as the strain, streaked and inoculated on the slant strain medium at 27°C, cultivated for 250 hours to obtain the activated strain; the formula of the slant strain medium is: malt extract 40 g / 100mL, peptone 4 g / 100mL, agar 10 g / 100mL, pH value is 5.4-5.6.

[0044] (2) Liquid strain culture: transfer the activated strain into the liquid seed culture medium at 27°C, culture on a shaker for 4.5 days to logarithmic phase to obtain the liquid strain, and the shaker rotates at 80 rpm. The formula of liquid seed culture medium is: glucose 5g / 100mL, peptone 0.5g / 100mL, yeast extract 0.1g / 100mL, KH 2 PO 4 0.1 g / 100mL, MgSO 4 0.15 g / 100mL, CaCl 2 0.01 g / 100mL.

[0045] (3) Liquid submerged fermentation: the liquid strain is connected to the fermenter equipped with liquid submerged fermentation medium for fermentation and culture, the volume ratio of liquid strain to liquid submerged fermentation medium is 1:8, and the process parameters of ...

Embodiment 2

[0048] (1) Strain activation: Inonotus obliquus as the strain, streaked and inoculated on the slant strain culture medium at 28°C, cultivated for 200 hours to obtain the activated strain; the formula of the slant strain culture medium is: malt extract 40 g / 100mL, peptone 4 g / 100mL, agar 10 g / 100mL, pH value is 5.4-5.6.

[0049] (2) Liquid strain culture: transfer the activated strain into the liquid seed culture medium at 28°C, cultivate on a shaker for 3.5 days to the logarithmic phase to obtain the liquid strain, and the shaker rotates at 140 rpm. The formula of liquid seed culture medium is: glucose 5g / 100mL, peptone 0.5g / 100mL, yeast extract 0.1g / 100mL, KH 2 PO 4 0.1 g / 100mL, MgSO 4 0.15 g / 100mL, CaCl 2 0.01 g / 100mL.

[0050] (3) Liquid submerged fermentation: the liquid strain is connected to a fermenter equipped with liquid submerged fermentation medium for fermentation and culture, the volume ratio of liquid strain to liquid submerged fermentation medium is 1:10, and the ...

Embodiment 3

[0054] (1) Strain activation: Inonotus obliquus as the strain, streaked and inoculated on the slant strain culture medium at 28°C, cultivated for 220 hours to obtain the activated strain; the formula of the slant strain culture medium is: malt extract 40 g / 100mL, peptone 4 g / 100mL, agar 10 g / 100mL, pH value is 5.4-5.6.

[0055] (2) Liquid strain culture: Transfer the activated strain into the liquid seed culture medium at 28°C, culture on a shaker for 4 days to logarithmic phase to obtain the liquid strain, and the shaker rotates at 100 rpm. The formula of liquid seed culture medium is: glucose 5g / 100mL, peptone 0.5g / 100mL, yeast extract 0.1g / 100mL, KH 2 PO 4 0.1 g / 100mL, MgSO 4 0.15 g / 100mL, CaCl 2 0.01 g / 100mL.

[0056] (3) Liquid submerged fermentation: the liquid strain is connected to the fermenter equipped with liquid submerged fermentation medium for fermentation and culture, the volume ratio of liquid strain to liquid submerged fermentation medium is 1:9, and the process...

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Abstract

The invention relates to the technical field of fermentation engineering, and discloses a submerged fermentation technology of producing immunological enhancement active materials of inonotus obliquus. The submerged fermentation technology of producing the immunological enhancement active materials of the inonotus obliquus comprises the main steps of culture activation, culture liquid cultivation, submerged fermentation and preparation of the immunological enhancement active materials of the inonotus obliquus and the like. The optimized submerged fermentation technology of producing the immunological enhancement active materials of the inonotus obliquus is high in content of the obtained immunological enhancement active materials, and good in activity of the obtained immunological enhancement active materials, and the obtained immunological enhancement active materials are beneficial for industrial application.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a process for producing immune-enhancing active substances of Inonotus obliquus by liquid submerged fermentation. Background technique [0002] China is the first country to use fungi to prevent and treat diseases, and fungi are an important part of traditional Chinese medicine in my country. Studies in recent years have shown that fungal polysaccharides are the main active substances of medicinal fungi, and have significant immune regulation functions. effect. Fungal polysaccharides are mainly metabolites isolated from basidiomycete and ascomycete fruiting bodies, mycelium and fermentation broth. So far, more than 200 kinds of polysaccharides with biological activity have been obtained from nearly 300 kinds of fungi at home and abroad. Among them, fungal polysaccharides with important value have been found in my country, such as: Yunzhi polysaccharide, Cordyce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A01G1/04C05G3/00
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