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Method for extracting triterpene substances from inonotus obliquus by use of quorum sensing molecules induction

A technology of quorum sensing and Inonotus obliquus, which is applied in the field of microbial fermentation and metabolism, to achieve the effect of promoting production and improving

Inactive Publication Date: 2018-03-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the experiment, it was found that the amount of triterpenoids obtained directly from Inonotus obliquus was very low, so it is hoped to find a more efficient method to increase the production of triterpenoids

Method used

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  • Method for extracting triterpene substances from inonotus obliquus by use of quorum sensing molecules induction
  • Method for extracting triterpene substances from inonotus obliquus by use of quorum sensing molecules induction
  • Method for extracting triterpene substances from inonotus obliquus by use of quorum sensing molecules induction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Preparation of solid medium

[0045] The bran is added into water according to the concentration of 5% by mass, and after boiling for 0.5 hour, remove the large particles by filtering with eight layers of gauze to obtain the bran water extract; then add PDA synthetic medium (commercially available), after mixing evenly, the After boiling, it was divided into glass tubes, 10 mL per tube, and then sterilized at 121 ° C for 20 min. After sterilizing, turn the inclined surface until the inclined surface solidifies.

[0046] Wherein, the mass percentage of raw materials is composed of: 1% of potato extract powder, 2% of glucose, 1.8% of agar, 0.01% of chloramphenicol, and the balance is bran water extract.

[0047] 2. Activation of bacteria

[0048] Inonotus obliquus CFCC 83414 was inoculated on the slope, and cultured in an incubator at 25°C for about 15 days until it covered the entire slope.

[0049] 3. Preparation of liquid medium

[0050] The bran is added into w...

Embodiment 2

[0078] Referring to the fermentation process of Inonotus obliquus in Example 1, in the stage of adding farnesol, 10 μL of 1M farnesol working solution was added to each bottle, so that the concentration of farnesol in the fermentation broth was 100 μM. Other conditions are with embodiment 1.

[0079] The detection method of triterpenes and betulinic acid concentration is the same as that in Example 1.

[0080] In the experimental group, farnesol was added in the middle stage of fermentation, and in the control group, DMSO with the same concentration was added. The measurement results showed that the contents of triterpenoids in the freeze-dried cells of the control group and the experimental group added with farnesol were 13.402 and 14.949 mg / g, and the contents of betulinic acid were 4.0265 and 3.2706 μg / g respectively; The contents of triterpenoids in the fermentation broth of the experimental group and the experimental group were 14.225 and 11.473mg / 100mL, and the contents...

Embodiment 3

[0082] Referring to the fermentation process of Inonotus obliquus in Example 1, in the stage of adding farnesol, 15 μL of 1M farnesol working solution was added to each bottle, so that the concentration of farnesol in the fermentation broth was 150 μM. Other conditions are with embodiment 1.

[0083] The detection method of triterpenes and betulinic acid concentration is the same as that in Example 1.

[0084] In the experimental group, farnesol was added in the middle stage of fermentation, and in the control group, DMSO with the same concentration was added. The measurement results showed that the contents of triterpenoids in the freeze-dried cells of the control group and the experimental group added with farnesol were 13.402 and 15.545mg / g, and the contents of betulinic acid were 4.0265 and 13.7273μg / g respectively; The contents of triterpenoids in the fermentation broth of the experimental group and the experimental group were 14.225 and 10.426mg / 100mL, and the contents ...

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Abstract

The invention discloses a method for extracting triterpene substances from inonotus obliquus by use of quorum sensing molecules induction, wherein the method comprises the following steps: adding activated inonotus obliquus CFCC 83414 into a liquid medium, and performing fermentation culture for 8-14 days at 24-26 DEG C; adding quorum sensing molecules in the 5th-8th days of the fermentation; after the fermentation, separating thalli and fermentation liquid; performing aftertreatment to separate and purify triterpene substances from the thalli and fermentation liquid. According to the method disclosed by the invention, quorum sensing molecules are added in the fermentation metaphase to stimulate growth of inonotus obliquus; the method does not need introduction of a gene engineering strainor additional enzyme, is simple in aftertreatment, and can effectively increase the yield of triterpene substances in inonotus obliquus.

Description

technical field [0001] The invention relates to the field of microbial fermentation and metabolism, in particular to a method for extracting triterpenes from Inonotus obliquus by using quorum sensing molecules to induce induction. Background technique [0002] Inonotus obliquus (Inonotus obliquus) is a very rare and valuable medicinal fungus, which belongs to the phylum Fungi, Basidiomycotina, Phytomycetes, Phytomycetes, Polyporaceae, and the genus Phytophthora. It is mainly distributed in Russia, Finland, Poland, Japan's Hokkaido and other regions in the northern hemisphere at latitudes 40°-50°, as well as in the Daxing'an Mountains of Heilongjiang and the Changbai Mountains of Jilin in my country. Since the 16th century, folks in Russia, Poland, Finland and other countries have widely used Inonotus obliquus to treat various intractable diseases, such as various cancers, heart disease and diabetes. [0003] Inonotus obliquus is a wood-rot fungus that grows in cold regions ...

Claims

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Application Information

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IPC IPC(8): C12P33/00C12P5/02C12N1/14C12N1/38C12R1/645
CPCC12N1/14C12N1/38C12P5/026C12P33/00
Inventor 陈启和李豪吴嘉南牛永武
Owner ZHEJIANG UNIV
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