Inonotus obliquus deep culture method

Abstract

The invention relates to an inonotus obliquus deep culture method, comprising the following steps: 1. slant culture; 2. primary, secondary and tertiary seed culture; 3. deep fermentation culture; wherein, the deep fermentation culture has the following conditions: (1) fermentation culture medium consists of 0.1-10% of corn saccharification liquid, 0.1-10% of soluble starch, 0.1-5% of yeast extract powder, 0.1-5% of peptone, 0.01-1% of KH2PO4, and 0.01-1% of MgSO4; (2) fermentation cycle is 3-15d; (3) culture temperature is 20-35 DEG C; (4) initial pH value of culture medium is 4-8; (5) rotation speed of table concentrator is 50-200r / min; (6) inoculation quantity is 5-20%; and (7) liquid volume is 1 / 10-4 / 5. The invention uses fermentation engineering technology to produce inonotus obliquus, obtains quantities of reproduced strains through the primary, secondary and tertiary seed culture, makes up the defect of insufficient productivity natural bacteria, has obvious economic significance, effectively avoids contamination of microorganism as the fermentation is carried out under the aseptic condition, and provides beneficial condition for preparing the inonotus obliquus.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a deep-layer culture method for Inonotus obliquus. Background technique [0002] Inonotus obliquus (Fr.) Pilat is a very rare and valuable medicinal fungus. Blackbirchtinder (tuchwood), birch mushroom, "カバノアナタケ", etc. taxonomically belong to Basidiomycotina, Hymenomycetes, Aphyllophorales, Polyporaceae, Brown Lyceum Poria hypobrunnea Petch. [0003] The wild species of Inonotus obliquus (Fr.) Pilat has high medicinal value only when it grows on live birch trees for 10-15 years. North America, Finland, Poland, Russia, China (Heilongjiang, Changbai Mountain area of ​​Jilin Province) and Japan (Hokkaido) and other countries. Inonotus obliquus is widely used in Russia, Northern Europe, Japan, the United States, and Korea to treat malignant tumors, diabetes, cardiovascular disease, liver disease, and AIDS. [0004] Due to the very limited resources of wild Inonotus obliquus, it i...

AI Technical Summary

Problems solved by technology

[0004] Due to the very limited resources of wild Inonotus obliquus, it is far from meeting the growing market demand; moreover, solid cultivation has disadvantages such as long cycle, high probability of pollution, and easy to be restricted by the surrounding environment.