The invention relate to the field of medical detection, particularly relates to a micro-fluid cytometer and a manufacture method thereof, and solves the technical problems that the traditional cytometer at present is huge in size, high in price and particularly high in mutual
infection rate. The micro-fluid cytometer mainly comprises a micro-fluid
chip and a specially-made external detection component, wherein a sample channel, a focusing detection channel and a sheath flow
liquid storage tank are arranged on the micro-fluid
chip; a sample preprocessing region and a buffer region for carrying out concentrating
processing on to-be-detected
cell sap are integrated in the sample channel; and a micro-column array is arranged in the sample preprocessing region. The manufacture method of the micro-fluid cytometer comprises the following steps of: manufacturing micro-fluid channels, and manufacturing and bonding a cover plate of the micro-fluid
chip. According to the micro-fluid cytometer and the manufacture method thereof, the products are integrally microminiaturized, the cost is low, the concentrating
processing on the to-be-detected
cell sap in the chip can be realized, the micro-fluid cytometer is disposable, each sample is measured at one time, and the problem of interaction effects among the samples is completely avoided.