The invention discloses a dual Tem-PCR quick detection method for salmonella and escherichia coli O78. According to the dual Tem-PCR quick detection method for the salmonella and the escherichia coli O78, invA gene primer of the salmonella and O-antigen gene primer of the escherichia coli are used for conducting dual Tem-PCR amplification directly on extracted mixing DNA after bacteria enrichment is conducted on a complex sample, and the result is interpreted through gel electrophoresis. A Tem-PCR technology and a dual PCR technology are combined, the problem of enrichment preference of the PCR is solved, the sensitivity degree is improved by 2 to 3 orders of magnitudes compared with an ordinary dual PCR detection, the detection level that the number of sample contaminated bacteria is a single digit can be reached, and the problem that a traditional dual PCR technology is prone to lead to false negative result. According to the dual Tem-PCR quick detection method for the salmonella and the escherichia coli O78, the salmonella and the escherichia coli O78 can be detected quickly, conveniently, excellently and sensitively, wide-range popularization is easy, the salmonella and the escherichia coli O78 can be used for bacteria identification, disease diagnosis and epidemiological investigation, and wide market prospect and comparatively large economic benefits are achieved.