114 results about "Blood culture" patented technology

A fluid transfer system has a holder that is made of a cylindrical member having a closed end and an open end. The closed end has fitted thereto a luer and a cannula that extends into the interior of the holder. The holder has a distal portion that has a first cross section and a proximal portion that has a second cross section. The cross section of the distal portion is smaller than the cross section of the proximal portion. The distal and proximal portions are joined by a shoulder. The fluid transfer system also includes an adapter that is inserted to the holder from the open end of the holder. The adapter has a base having a central opening and a cylindrical tube that extends from the central opening. The diameter of the cylindrical tube is slightly smaller than the diameter of the distal portion and is configured to accept a first type of fluid collection store such as a vacuum tube. With the adapter removed, the holder can accept a differently dimensioned fluid collection store such as for example a blood culture collection bottle.

A fluid transfer system has a holder that is made of a cylindrical member having a closed end and an open end. The closed end has fitted thereto a luer and a cannula that extends into the interior of the holder. The holder has a distal portion that has a first cross section and a proximal portion that has a second cross section. The cross section of the distal portion is smaller than the cross section of the proximal portion. The distal and proximal portions are joined by a shoulder. The fluid transfer system also includes an adapter that is inserted to the holder from the open end of the holder. The adapter has a base having a central opening and a cylindrical tube that extends from the central opening. The diameter of the cylindrical tube is slightly smaller than the diameter of the distal portion and is configured to accept a first type of fluid collection store such as a vacuum tube. A non-continuous wall extends from the base of the adapter to surround the cylindrical tube. The cross section of the wall is slightly smaller than the diameter of the proximal portion. At each section of the non-continuous wall there is formed a protuberance. A groove is formed about the inner surface of the proximal portion. When the adapter is fully inserted to the holder, the protuberances would snap fit to the groove so that the adapter is prevented from being inadvertently removed from the holder. With the adapter removed, the holder can accept a differently dimensioned fluid collection store such as for example a blood culture collection bottle.

A sample is tested for the presence of bacteria, such as in an automatic blood culturing apparatus. If bacteria are determined to be present, a bacteriophage-based bacteria identification process is performed to identify the bacteria present. A plurality of bacteria detection processes, such as a blood culture test and Gram stain test may be carried out prior to the bacteria identification process. A bacteriophage-based antibiotic resistance test or antibiotic susceptibility test is also conducted on the sample.

The present invention is directed to a method for separating, characterizing and / or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and / or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for the use of one or more identifier agents and interrogating the microorganism sample and / or said one or more identifier agents to produce measurements which characterizing and / or identifying the microorganism based on the produced measurements and / or the presence or absence of the identifier agent or a metabolized form of the identifier agent in the microorganism sample.

Generally provided are methods for rapid culture of microorganisms in a sample, including methods for growth and recovery of live microbial cells directly from a sample. Various features include enabling growth of microorganisms in a sample along with a reduction of sample debris that may interfere with microorganism detection, and reduction in toxicities that may inhibit microorganism growth. Further methods for selectively degrading non-viable microbial cells, are provided, for enhanced detection of viable microbial cells following a growth period.

The invention discloses a blood bacteria proliferation culture bottle used for a full automatic blood culture system, including: a bottle body of a sealed culture bottle, a sensing level arranged at the bottom of the bottle body, and polymeric adsorbent used for adsorbing antibiotic and culture medium which are placed on the sensing level, wherein, the culture medium includes 5 to 75g / L of TSB, 0.5 to 30g / L of yeast extract, 0.1 to 20g / L of glucose, 0.01 to 20g / L of sucrose, 0.001 to 0.5g / L of chlorhematin, 0.0001 to 0.05g / L of Vitamin K3, 0.001 to 5g / L of Vitamin B6, 0.1 to 30g / L of SPS, 1 to 75g / L antibiotic neutralizing agent, and the residue is distilled water or deionized water; and the sensing level contains 1 to 100g / L of an indicator. The culture bottle of the invention can eliminate the inhibitory action of the residual antibiotic in the blood to the growth of the microorganism, and simplify the production operation.

The invention discloses an in-vitro separation culture method for hippocampal neurons of an adult rat. The in-vitro separation culture method comprises the following steps of coating a culture plate; separating a rat brain; separating a hippocampus; preparing a cell suspension; further purifying the neurons; carrying out cell inoculation; and carrying out in-vitro primary culture. The invention aims at providing a high-purity and high-activity acquiring (separating and purifying) method for hippocampal neurons and an establishing method for a flow culture system suitable for long-term in-vitro culture of hippocampal neurons of the adult rat.

The invention discloses a tissue culture and rapid propagation method of oriental cherries. The tissue culture and rapid propagation method of the oriental cherries comprises the following steps: obtaining oriental cherry stem explants, carrying out induced culture on the oriental cherry stem explants, carrying out multiplication culture on oriental cherry cluster buds, carrying out rooting culture of the oriental cherries, carrying out seedling hardening on oriental cherry tissue culture seedlings and transplanting the oriental cherry tissue culture seedlings. Culture media prepared by the invention are used in induced culture, multiplication culture and rooting culture processes, and obtained seedlings easily take root, are healthy and strong and have resistance and strong disease and pest resistance; the seedlings are ordered and consistent, have high quality and are relatively easy to cultivate and manage. The tissue culture seedlings obtained by the invention have a strong adaptive capacity on a hot and humid environment and straight stems; and the quality is improved by 1-2 grades and the value is increased by 20%-40%.

The invention relates to asparagus parent breeding method, mainly solving the problems that rooting rate is low, root quality is poor and the survive rate of the regeneration plant is low in asparagus organ tissue culture process. The method includes the process that high quality asparagus stem section is selected, culture is carried out in culture medium solution, and asparagus seedling meeting transplant standard is bred. The culture medium solution is confected by the way that hormones corresponding to various culture stages are added on the basis of the basic culture medium solution, the culture includes induction culture, propagation culture, rooting pre-differentiation culture and rooting culture sequentially. Compared with the existing organ culture, the invention has high propagation rate, rooting rate reaches 100%, root system quality is good, and differentiation rate of strong fleshy tap root is improved to 96%, thus ensuring survival rate after transplantation.

The invention discloses an in vitro conservation method of a color calla idioplasm resource, pertaining to the vegetable idioplasm resource conservation and tissue cultivation technical field. The method comprises such steps as preparation of a culture medium, disinfection of an explant and inoculation and induction culture, multiplication culture, root culture, a corm intumescence culture, idioplasm conservation culture, restore culture, and the detection and utilization of genetic stability, etc. The method adopts the meristem tissue of a 0.5mm stem tip of the color calla as the explant, a large quantity of elements in the culture medium are reduced and mannitol and penicillin are added, and a protocorm seedling is conserved at 10 to 15 DEG C under the surroundings of light intensity of 1000Lx for 10h / d, and after 15 months, the contamination of Erwinia is eliminated, the survival rate reaches 100 percent; the growth and multiplication are still normal without genetic variation. The method of the invention is characterized by low cost and simple operation etc., and can be popularized and applied in the idioplasm conservation field of the color calla.

The invention relates to a fast propagation method for aquatic plant lotus flowers. According to the method, in vitro tissue culture is firstly adopted for obtaining tissue culture seedlings, and the in vitro tissue culture comprises the following steps that: (1) lotus flower seeds are used as initial raw materials, after the lotus flower seeds are subjected to sterilization and disinfection treatment, plumules are taken out under the aseptic condition and are inoculated into a basic culture medium to be cultured for 2 to 3 weeks, and aseptic seedlings are obtained; (2) the hypocotyl upper parts and cotyledons of the aseptic seedlings containing growth point parts are cut off and are inoculated into a propagation culture medium, the light illumination culture is carried out for 3 to 4 weeks, and tissue culture calluses are obtained; (3) buds with the length being 2 to 3cm in the tissue culture calluses obtained in the second step are cut off and are inoculated into a strong seedling culture medium to be cultured; and (4) after the seedlings in the third step grow to 4 to 6cm, the seedlings are transplanted onto a rooting culture medium for carrying out rooting culture, and the tissue culture seedlings are obtained. The fast propagation method provided by the invention solves the problems of few lotus root resources and long propagation period in the prior art, and a new path is provided for meeting the market requirements.

The invention relates to worm grass fungus and the artificial culture method thereof, which belongs to the micro organism technology field. The worm grass fungus disclosed by the invention is high essence worm grass fungus Paecilomyces tenuipes(Peck)Samson=P. tenuipes, the artificial culture method comprises the strain activation, the mycelium culturing through liquid culture method and the fruiting body culturing with silkworm pupa. The invention adopts the main steps that the silkworm pupa is adopted as the host plant of the artificial cultured worm grass, and the fruiting body culturing with silkworm pupa is divided into two steps as follows: earlier stage (o to 5-15 days) and later stage (5 to 15 days until collection), lucifuge culturing is adopted in the earlier stage, illuminantion, abundant oxygen supply and more than 75 percent of ambient humidity are ensured in the later stage, and compared with the earlier stage, the ambient temperature of the later stage is obviously reduced; the invention can meet the scale production of the artificial worm grass.

The invention discloses a blood microorganism culture flask which comprises a flask body, a flask cover, chemoreceptors, a liquid culture medium and an absorption resin, wherein an asepsis environment is provided in the culture flask; the flask body is a cylindrical plastic container; a layer of chemoreceptors are arranged at the bottom of the flask body; the chemoreceptors are high-molecular polymer semipermeable membranes which are solidly adhered to the flask bottom. By utilizing the culture flask provided by the invention, a technical difficult problem of a full-automatic blood culture instrument is solved, full-automatic culture and detection on blood microorganism are realized, the detection sensitivity is improved, and the positive detection time is shortened.

The invention discloses a tissue culture method for improving the in-vitro regeneration efficiency of a common head cabbage. The tissue culture method comprises the steps of at first, taking explant with high regeneration and induction rate; sterilizing by using sodium hypochlorite; carrying out dark culture on a tender leaf on an induction culture medium for 16-20 hours, and then carrying out light culture; directly carrying out the light culture on a tender stem section with an axillary bud on the induction culture medium; transferring induced bud or cluster buds on an enrichment culture medium for enrichment culture; taking a strong single bud for rooting culture; removing a sealing film at room temperature for plantlet hardening after the strong single bud is rooted; washing off the culture medium from the root; and transplanting the bud in a prepared hole plate. With the adoption of the tissue culture method provided by the invention, the problem that explant sterilization time is different can be solved, and the problem that the regeneration coefficient in an in-vitro regeneration and induction process of the common head cabbage is low can be solved. With the adoption of the tissue culture method provided by the invention, the most efficient regeneration system of the common head cabbage can be established on the aspects of the sterilization time, an explant taking part, and different hormone combinations, and lays the foundation for the storage of genetic resources of the common head cabbage and the development of breeding of common head cabbage genetic engineering.

The invention relates to a bartonia body solid-liquid double-phase slant culture medium as well as a preparation method and an application method thereof. The slant culture medium comprises a slant solid culture medium and a liquid culture medium, wherein the slant solid culture medium comprises beef leach liquid, starch, sodium chloride, agar, defibrinated sheep blood and distilled water; and the liquid culture medium comprises trypticase hydrolysate, soybean protein and papayotin hydrolysate, glucose, sodium chloride, di-potassium phosphate and distilled water. The bartonia body solid-liquid double-phase slant culture medium prepared by the method approximately requires 48 hours to reach 107 CFU / ml, the growth speed is increased by 2.5 times, the cost is saved by 500 times (blood plate culture approximately requires 5 days, and reaching 107 CFU / ml requires 100 flat plates), and the defects of slow bacteria growth, time taking, labor taking, high cost and the like of the traditional bartonia body invitro culture method are remedied .

The invention provides a reagent for separating bacteria from positive blood. The reagent comprises an agent A, an agent B and an agent C, wherein the agent A is used for dissolving the hemocyte froma blood sample of a blood culture; the agent B is used as a cleaning agent; the agent C is used for breaking the pathogenic bacteria cytoderm and promoting the protein release in the cells; and the agent A is at least one of saponin solution and SDS or other surface active agent capable of cracking the hemocyte. The invention also provides a method for separating bacteria from positive blood and the method for identifying bacteria. The method comprises the following steps: cracking the hemocyte by using the agent A; cleaning the cell fragments and impurities by using the agent B; breaking thepathogenic bacteria cytoderm by using the agent C, releasing the bacteria protein, taking the supernate containing the bacteria protein and settling a sample to be identified; dropping the sample to be identified into a vacant side on a target plate; drying under room temperature and then covering a substrate on the sample; drying under room temperature; and putting onto a machine for detecting. The bacteria can be quickly identified, the result can be acquired within half an hour, the detection result is high in reliability and the interference is less.

The invention relates to equipment for carrying out bacterial culture and automatic implantation on a blood sample and particularly relates to an automatic implantation type blood culture instrument. According to the automatic implantation type blood culture instrument, three culture tanks are arranged, a first culture tank is used for arranging blooding culture bottles and corresponding detection devices, and a streak inoculation site, a delivery mechanical arm and a streak mechanical arm are arranged among the first culture tank, a second culture tank and a third culture tank, so that the timed implantation capacity of a positive sample is greatly improved. Compared with products in the prior art, the automatic implantation type blood culture instrument has the characteristics that by virtue of the delivery mechanical arm and the streak mechanical arm, the streak inoculation and isolated culture procedures of a positive blood sample can be automatically finished by the instrument; the implantation of the positive sample is realized under a culture environment, and the timed implantation capacity of the sample is improved; by implanting the sample to a solid culture medium under the culture environment, the positive rate of the implantation is increased; the labor force of professionals in a microbiological lab is liberated, and the blood culture quality is improved.

The invention mainly relates to the mass spectrometric identification of pathogens in blood cultures from blood-stream infections (septicemia). The invention provides a method with which microbial pathogens can be separated in purified form from blood after a relatively brief cultivation in a blood culture flask, without any interfering human proteins or any residual fractions of blood particles such as erythrocytes and leukocytes, and can be directly identified by mass spectrometric measurement of their protein profiles. The method is based on the use of relatively strong tensides to destroy the blood particles by dissolving the weak cell membranes and most of the internal structures of the blood particles; in spite of the fact that tensides are regarded as strong ionization inhibitors in MALDI and other ionization processes required for mass spectrometric measurements. This method allows unknown pathogens to be obtained in their pure form by centrifuging or filtration and to be identified on the taxonomic level of species or subspecies. Problems with DNA from high levels of leukocytes can be resolved by special measures. After sufficient cultivation, the identification in a mass spectrometric laboratory takes only half an hour.

The invention relates to methods and instruments for the rapid detection and rapid mass spectrometric identification of microbial infective agents in blood or other body fluids. The invention recognizes that blood is not a good environment for the cultivation of microbes and provides a method which (a) largely destroys or dissolves the human particles in body fluids, such as erythrocytes and leukocytes in blood, without impairing the ability of the microbes to reproduce, (b) separates the microbial pathogens from the fluid, (c) cultivates them in a nutrient broth which contains none of the antimicrobial components of the body fluids, (d) separates them from the nutrient broth, and (e) identifies the microbes by a mass spectrum of the microbial proteins. The dissolution of the human particles also releases the microbes nesting in macrophages. The cultivation in an optically clear nutrient broth with optimum composition not only accelerates the propagation of the microbes compared to all other cultivation methods, but also makes it possible to continuously measure their quantitative growth starting from a low microbe density. This firstly allows the mass spectrometric identification to be carried out at the earliest possible time, secondly provides a positive detection of microbes far ahead of their identification, which can be lifesaving for the patient; and thirdly makes it possible to start the determination of resistances early.

A system that indicates the presence or absence of microorganisms in fluid food products. The system has a bottle for receiving sample to be tested. The bottle has a sensor that will monitor and detect changes in at least one sample parameter, but no additives that contain nutrients that support microbial growth. The bottle is placed in an incubator and the sensor in the bottle is monitored for changes. The incubator is programed so that, if the sensor detects that the value of the monitored parameter has reached a certain value, then the sample is determined to be positive for microbial growth.

The invention relates to a tissue culture and rapid propagation method of curcuma longa. The method comprises the following steps: taking an underground tuber of the curcuma longa as an explant, cleaning, disinfecting, cleaning again, absorbing the water on the surface of the tuber, and putting the explant into an MS germination culture medium for inducing germination to obtain sterile test-tube plantlets; putting the test-tube plantlets into an MS clustered bud culture medium for breeding cultivation to obtain clustered buds; putting the clustered buds into an MS strong seedling culture medium for cultivation to obtain robust plants; moving the robust plants into a 1 / 2 MS rooting culture medium for rooting culture to obtain complete seedlings with roots; exercising the complete seedlings with roots for 6-10 days, transplanting the seedlings into a sand bed to grow for 27-33 days at first, and then transplanting the seedlings into a big field. The multiplication coefficient of the clustered buds obtained by the culture method is 10-15 times, the rooting percentage of the obtained tissue culture seedlings is 100%, and the survival rate of the transplanting seedling bed is more than 95%; the problem of industrialized seedling production of the curcuma longa can be effectively solved.

The invention provides a primer group and a method for detecting four kinds of bacteria by means of multiple polymerase chain reaction (PCR). The primer group comprises phoA gene primer pairs of escherichia coli, mdh gene primer pairs of klebsiella pneumoniae, femA gene primer pairs of staphylococcus aureus and mecA gene primer pairs of methicillin resistant staphylococcus aureus. A detection method comprises the steps of firstly, extracting a DNA target template; performing multiple PCR amplification on the extracted DNA target template by using the primer group; finally, detecting and analyzing the obtained multiple PCR amplification product by means of gel electrophoresis. According to the detection method, the four kinds of bacteria can be rapidly detected in the same reaction system; compared with the traditional unique PCR or blood culture detection method, the detection method provided by the invention has the advantages of time saving, simpleness and convenience.

The invention discloses a full-automatic blood culture instrument. The full-automatic blood culture instrument comprises at least one full-automatic blood culture module that can be cultured independently; the full-automatic blood culture module includes a box, a temporary culture bottle storage module, a bottle push module, a bottle rotating module, an XYZ triaxial manipulator, a culture detection module, a positive bottle output area, a negative bottle drawer and culture bottles. By the arrangement, the full-automatic blood culture function is realized, time is saved, the work flow is standardized, and manual error is avoided; meanwhile, positive results can be output at the first time, so that patients can get corresponding treatment in time; the full-automatic blood culture instrumentis simple in structure and can reduce the probability of failure; the full-automatic blood culture instrument has strong expansibility which is performed by simply connecting the full-automatic bloodculture modules with only one hose computer equipped with a control system, so that needs of larger scale culture can be met.

The invention relates to a blood culture instrument comprising a fixing frame (13), a swinging frame (8), a motor (4) and a speed reducer (5), wherein the fixing frame (13) is arranged on a base (12); the swinging frame (8) is articulated in the fixing frame (13), and the motor (4) and the speed reducer (5) are fixedly arranged on the base (12); the swinging frame (8) is of a hollow rectangle structure, the swinging frame (8) is arranged in the fixing frame (13) in a swinging manner, a first connection rod (6) is articulated with the swinging frame (8) through a second connection rod (7) on the swinging frame (8) and is also articulated on the speed reducer (5); an optical detection sensor is arranged on the swinging frame (8) and swings along the swinging of the swinging frame (8). Therefore, according to the blood culture instrument disclosed by the invention, a situation of inaccurate detection caused by nonalignment in the movement of the motor and difference in positions of detection holes is avoided; in addition, each detector is mutually independent, and the position of each detector is found out without depending on the movement of the motor, so that the time required by the movement of the motor is saved, and the detection speed is increased.

The present invention belongs to the technical field of cell culture and particularly relates to a Chinese mitten crab blood cell culture medium and a culturing method. The Chinese mitten crab blood cell culture medium comprises a basic culture medium and added components, wherein the basic culture medium is selected from a TC-100 basic culture medium; and the added components and concentrations thereof are as follows: 1%-10% of a buffer solution, 1g / L-2g / L of calcium chloride, 1g / L-2g / L of alkali metal chloride, 1.5g / L-2.5g / L of glucose and 1%-1.5% of antibiotic. The culture medium significantly improves survival rate of Chinese mitten crab blood cells in in-vitro culture and prolongs duration of the blood cell culture in vitro, and the blood cells cultured in vitro can maintain good cellmorphology.

The invention relates to methods and instruments for the rapid detection and rapid mass spectrometric identification of microbial infective agents in blood or other body fluids. The invention recognizes that blood is not a good environment for the cultivation of microbes and provides a method which (a) largely destroys or dissolves the human particles in body fluids, such as erythrocytes and leukocytes in blood, without impairing the ability of the microbes to reproduce, (b) separates the microbial pathogens from the fluid, (c) cultivates them in a nutrient broth which contains none of the antimicrobial components of the body fluids, (d) separates them from the nutrient broth, and (e) identifies the microbes by a mass spectrum of the microbial proteins. The dissolution of the human particles also releases the microbes nesting in macrophages. The cultivation in an optically clear nutrient broth with optimum composition not only accelerates the propagation of the microbes compared to all other cultivation methods, but also makes it possible to continuously measure their quantitative growth starting from a low microbe density. This firstly allows the mass spectrometric identification to be carried out at the earliest possible time, secondly provides a positive detection of microbes far ahead of their identification, which can be lifesaving for the patient; and thirdly makes it possible to start the determination of resistances early.

The invention discloses a tissue culture method for Punica granatum and a culture medium. The culture method comprises explants picking, induction of differentiated seedlings, proliferation culture of differentiated seedlings, rooting culture of differentiated seedlings and transplantation of tissue culture seedlings to the field. The culture medium comprises an induction medium, a proliferation medium and a rooting medium. A Punica granatum tissue culture induction method is also included. The three mediums are respectively prepared and applied correspondingly. By the method of the invention, propagation coefficient of Punica granatum is raised; some insufficiencies of a traditional tissue culture technology are compensated; contamination rate of explants is reduced; and differentiation rate and transplanting survival rate of buds are enhanced. by the preparation method of the induction medium, the proliferation medium and the rooting medium, culture period of tissue culture seedlings can be shortened, there is no variation, and uniformity of seedlings is high. In addition, proliferation is rapid, propagation coefficient is raised. Rooting rate is high, there is no callus between roots and seedlings, and roots are all normal roots. By the cooperation of the three mediums, survival rate of domestication and proliferation coefficient can be greatly raised, and cost of tissue culture seedlings is reduced.