128 results about "Rat brain" patented technology

The invention provides a robot navigation map construction method based on rat brain hippocampus spatial cells. According to an information transfer loop of spatial navigation related cells in a hippocampus structure of a mammal, a robot acquires a current autonomic movement clue and color depth image information through environment exploration. The autonomic movement clue progressively forms coding to the spatial environment through path integration and characteristic extraction of the spatial cells in the hippocampus structure. The place field of place cells is gradually formed in an exploration process and furthermore covers the whole spatial environment, thereby forming a cognitive map. Meanwhile, Kinect acquires color depth image information of a view scene right in front of the current position as an absolute reference for performing closed-loop detection on the path and correcting a path integration error. At a closed-loop point, a system resets spatial cell discharging activity and performs correction on the path integration error. Nodes on a final navigation map comprise place cell mass coding information, corresponding visual cues and place topological relationships.

The invention discloses a robot simulated navigation method based on rat brain-hippocampal navigation, and belongs to the technical field of robot motion navigation. The method is characterized by that a robot CPU (Central Processing Unit) predefines a grid cell layer cell layer G and a place cell layer P by imitating rat brain and hippocampal tissues, motion information including a motion direction phi and a velocity v is collected when a robot explores an environment, the collected motion information is input into the grid cell layer, the motion information is integrated by using a shock interference model in the grid cell layer to form a grid cell layer response, a connection weight value W of the grid cell layer cell layer G and a place cell layer P is calculated by using a neural network algorithm to form a location cell-grid cell response network, and the robot perception for a space namely is stored in the form of a neural network response. The robot controls a motion decision module to implement motion decision-making according to space information stored in a cognitive map, and the navigation method disclosed by the invention independently obtains a better spatial cognitive effect during an exploration motion of the robot, and can be applied to industrial robots, service robots and the like.

The invention discloses a device used for rat brain tissue slicing and layering. The device comprises a slicing module, wherein the slicing module is provided with a plurality of cutter grooves with equal spacing, and fresh rat brain tissues are placed on the slicing module in slicing operation. The device is characterized in that the slicing module is embedded in a rectangular cavity on an operation desk, a cutter with a tool rest is arranged above the slicing module, two blades which are mutually parallel and vertical downwards are arranged below the tool rest, spacing between the two blades is just equal to distance between two cutter grooves of the slicing module, the cutter is connected with the upper end of a vertical arm, and the lower end of the vertical arm is connected with a mechanism which can move along the front length direction of the operation desk for positioning.

The invention relates to the field of medicine, and discloses a novel application of stilbene compounds in preparation of drugs for curing ischemia stroke in the acute phase. The effective time windows of rhubarb stilbene glucoside and piceatannol can last for 6 hours; the rhubarb stilbene glucoside and piceatannol can decrease the water content in the rat brain tissues during the cerebral ischemia process of a rat, and improve the rat nerve function scores. The mechanism is that rhubarb stilbene glucoside and piceatannol have strong functions of oxidation resistance and clearance of oxygen free radicals, and have a brain protection function. The piceatannol has stronger functions of oxidation resistance and clearance of oxygen free radicals than those of the rhubarb stilbene glucoside in vitro. So the invention provides applications of rhubarb stilbene glucoside and piceatannol in preparation of drugs for curing ischemia stroke in the acute phase.

The invention discloses an in-vitro separation culture method for hippocampal neurons of an adult rat. The in-vitro separation culture method comprises the following steps of coating a culture plate; separating a rat brain; separating a hippocampus; preparing a cell suspension; further purifying the neurons; carrying out cell inoculation; and carrying out in-vitro primary culture. The invention aims at providing a high-purity and high-activity acquiring (separating and purifying) method for hippocampal neurons and an establishing method for a flow culture system suitable for long-term in-vitro culture of hippocampal neurons of the adult rat.

The invention relates to a pharmaceutical composition for preventing and treating Parkinson's disease. The pharmaceutical composition consists of active ingredients and medically acceptable auxiliary materials, and is characterized in that the active ingredients consist of the following components in percentage by weight: rhizoma acori graminei volatile oil extracted from rhizoma acori graminei accounting for 25-45 percent and tortoise plastron water extract extracted from tortoise plastron accounting for55-75 percent. By adopting the pharmaceutical composition, neurological integrals of Parkinson's model rats can be obviously improved, the content of monoamine neurotransmitters in rat brains is increased, reduction of tyrosine hydroxylase in brain is inhibited, and the effect of preventing and treating the Parkinson's disease is obvious.

The invention discloses an experiment method for studying the effect of tenuigenin on synaptic transmission of rat hippocampal neuron. The method adopts a micro control instrument, a microscopic, and a recording electrode to monitor the spontaneous current state of neuron in tenuigenin with different concentrations, and then the monitor current data is analyzed so as to obtain the influence mechanism and effect of tenuigenin on rat hippocampal neuron. The method adopts a whole-cell patch-clamp technology to research the effect of tenuigenin on spontaneous postsynaptic current in the rat brain hippocampal CA1 area so as to disclose the regulating effect and mechanism of tenuigenin on neuron synaptic transmission, thus the requirements of scientific research and drug development are fulfilled, and at the same time a basis is provided for the further research on the signal transmission mechanism of neuron. The method can be widely used in the pharmaceutical theory researches on neuron, the experiment method and equipment design are scientific and reasonable, and requirements of scientific research can be met.

The invention discloses a water-soluble aggregation-induced luminescent probe as well as a preparation method and an application thereof and belongs to the technical field of fluorescent probes. The fluorescent probe has the structural general formula as shown in the description, wherein in the general formula, R1 is tertiary amine, Ar1 is a benzene ring or a naphthalene ring, Ar2 is onium salt containing pyridine or quinoline and Ar3 is an aromatic ring or a heteroaromatic ring. The fluorescent probe provided by the invention can be used for directly observing Abeta deposited plaques and hasexcellent targeting ability and sensitivity, so that the Abeta deposited plaques can be rapidly and accurately detected; and the Abeta deposited plaques of AD rat brain tissues can be subjected to washing-free rapid detection and fibrin, especially egg-white lysozyme fibers, can be quantitatively detected according to the intensity of fluorescent light.

The invention discloses a rat-intracranial-implantation microfilament array electrode suitable for an experiment and a manufacturing method thereof. The electrode is formed by a plurality of electrode microfilaments, a dosing conduit and the like. The electrode microfilaments are arranged in parallel and form a microfilament measurement electrode array and a microfilament stimulation electrode array respectively. The electrode manufactured through using the method of the invention can simultaneously realize three functions of experiment rat brain electric signal recording, electrical stimulation and drug stimulation. According to a specific demand, one or any two functions can be selected. And self-manufacturing can be completed with low cost.

The invention discloses a detection method for determining monoamine neurotransmitters based on derivatization. According to the detection method, the monoamine neurotransmitters and derivative reagent n-butanal and the like generate derivative reaction, and an obtained derivatization product is analyzed and detected by an ultrahigh-phase liquid chromatogram-mass spectrum combined system; the monoamine neurotransmitters include dopamine, noradrenaline and 5-hydroxytryptamine. Derivatization reaction conditions of the method have the advantages of simpleness, gentleness, quickness and environmental friendliness; the method can process samples in batch and has the advantages of high analyzing speed, low analyzing method quantitative lower limit and good linear range, precise and accuracy; simultaneous quantitative analysis on the dopamine, the noradrenaline and the 5-hydroxytryptamine in trace rat brain microdialysate is achieved.

The invention discloses a micro-fluidic chip for in-vivo on-line simultaneous detection of ascorbic acid and magnesium ion and a preparation method and applications thereof. The micro-fluidic chip comprises: a template with a micro channel and a conductive glass substrate which are connected closely; working electrodes which are disposed at positions corresponding to the micro channel on the substrate in a direction of the micro channel, and are arranged in turn with an amount being the same as the amount of components to be detected; a liquid introducing channel and an inlet are disposed at one port of the micro channel on the template, and a liquid extracting channel and an outlet are disposed at the other port; a counter electrode is disposed at the port of the micro channel where the liquid outlet is disposed; on the template, a reference electrode is disposed at a position corresponding to the micro channel between the main liquid introducing channel and the liquid extracting channel. With the micro-fluidic chip, cross interference between ascorbic acid and magnesium ion during the simultaneous detection is eliminated; furthermore, a micro-dialysis in-vivo on-line detection system is combined so as to realize the simultaneous on-line electrochemical detection of ascorbic acid and magnesium ion in rat brains.

The invention discloses a metal nanoparticle composite ICP (inductively coupled plasma) material and application thereof in dopamine content detection. The metal nanoparticle composite ICP material is an infinite coordination polymer comprising an Ag nanoparticle-modified repeating structural unit shown as formula I. By use of highly-sensitive and highly-selective fluorescent recognition functions of the surface modified rough Ag nanoparticle infinite coordination polymer ICP material on dopamine, direct fluorescence detection of the dopamine content in rat brain dialysis fluid can be realized. The method has the advantages of high sensitivity, good selectivity, fast response, low cost, less sample demand, and the like, and more importantly, the method has the advantages of simple operation and high stability, and can be applied to the determination of dopamine basic values in living body.

The invention provides a rat cerebral electrical stimulation device and electrode fixing bases thereof. The electrical stimulation device comprises an electric simulator, two electrode fixing bases, and an electrode wire capable of electrically connecting the electric simulator and the electrode fixing bases, wherein each electrode fixing base comprises an insulated fixing section, an electrode inserting section, a conductive contact section and a bolt; two or more bolt limiting holes are formed in the insulated fixing section in a center symmetry manner; the electrode inserting section is fixedly arranged at the center position of the upper end surface of the fixing section; a conductive electrode inserting hole is formed in the center of the inserting section; the conductive contact section is arranged at the center position on the lower end surface of the fixing section; the upper end of the contact section penetrates through the fixing section and is connected with the electrode inserting hole; the lower end of the contact section is arranged to contact a cerebral dura mater of a rat after passing through the rat brain; and the bolt is matched with the bolt limiting hole and is used for fastening the fixing section on a rat skull. According to the rat cerebral electrical stimulation device, a simulation electrode can be stably arranged on the rat skull in a plug and play manner, and the technical problems that an electrode in the prior art easily falls off and the electrode inserting depth is difficult to control are solved.

The invention relates to the field of medicine, in particular to a polypeptide capable of simulating the brain-derived-neurotrophic-factor physiological function and reducing depressive symptoms. The sequence is SEQ ID NO:1, and the SEQ ID NO:1 is a bran-new sequence. The polypeptide has the advantages that the BDNF content of chronic inscrutable stress model rat brain tissue can be increased. The in-vivo pharmacological result shows that depressive symptoms of acute and chronic depression model animals can be improved through the polypeptide, and the polypeptide can serve as an effective candidate treating method for clinical depression.

The invention relates to a rat brain section microscopic image segmentation method based on a markov random field theory. Gaussian mixed distribution is trained through the existing tagged image and used in characteristic field modeling, image characteristics are simulated correctly, great guiding significance for random field modeling is obtained, convergence times of an iterative algorithm are greatly reduced, and the accuracy of a segmentation result is improved. In addition, in order to solve the problem that local neighborhood characteristics of a traditional 8 neighborhood pixel model traced image are too rough, the method introduces a pixel gray value and a distance between pixels into a Potts model, a new potential-energy function is defined, image local information is described correctly, and the accuracy of a segmentation result is improved.

The invention discloses a method for preparing an immobilized metal affinity material by using a chelating agent 5-pyridoxal phosphate, and an application of the immobilized metal affinity material in sample pretreatment. The method comprises the following steps: firstly selecting several common nano materials to carry out chemical modification so as to prepare amino modified nano materials to obtain Y-NH2; grafting the 5-pyridoxal phosphate on the Y-NH2 to obtain a surface modified phosphate radical Y-NH2-PLP; and finally immobilizing metal ions on the phosphate radical to obtain Y-NH2-PLP-M<n+>. The modification method of the invention is simple and time-saving, and does not destroy the morphological characteristics of the base material, and the modification is used for realizing the functionalization of the base material, thereby increasing the practicability of the base material. The Y-NH2-PLP-M<n+> can be directly used as extraction material for the sample pretreatment of complex biological samples, the material is separated from the sample in the extraction process through centrifugal or external magnetic field action, and the immobilized metal affinity material has been successfully applied to extraction of phosphorylated polypeptide of standard peptide mixed liquor, milk enzymatic hydrolysate, human serum samples and rat brain lysate.

The invention relates to seed selection of high-immunogenicity rabies virus fixed strain and application thereof in vaccine development, which belongs to the field of biotechnology. The method comprises the following steps of: (1) augmentation and purification of rat brain virus strain; (2) culturing of the rat brain virus adaptive to Vero cell; (3) augmentation of the virus in nude rat brain tissue by Vero cell culture; (4) alternate transfer of the virus in nude rat brain tissue and Vero cell; (5) continuous transfer of the virus in Vero cell; (6) monoclonal purification, screening, storage and strain culturing of the virus; and (7) transfer stability analysis of the bred virus strain, wherein the obtained optional virus strain is applied as a virus strain for vaccine production. The new rabies virus fixed strain bred by the method can be augmented at high titer in the Vero cell; the augmented virus has higher immunogenicity than the present rabies virus fixed strain; stabilities of biology, genetics and other characteristics are still maintained after a plurality of times of continuous transfer in Vero cell, so the method provided by the invention can be used for large-scale industrial production of rabies vaccine with better immunoprotection effect and higher safety.

H-ferritin receptors on endothelial cells In rat brain rat brain microvasculature are a means for transporting iron across the blood brain barper. A method for treating an iron deficiency disorder in the brain of a patient comprises administepng to a patient in need an H- ferritin-iron complex. A method for using H-ferritin as a targeting moiety comprises attaching H-ferritin to a liposome targeted to the brain or cells within the brain. A method for treating an iron overload disorder in a patient comprises administering to a patient a multi subunit ferritin complex at less than 100% iron binding capacity.

The invention discloses a rat single-cage fixing device for electromagnetic exposure experiments. The fixing device comprises a rat cage rack, two door control baffles and an adjusting baffle, wherein a cuboid cavity is defined in the rat cage rack, the two ends, in the length direction, of the cavity are open, and the rat cage rack is provided with an installation through hole communicated with the cavity; the two door control baffles can be movably connected with the two ends of the rat cage rack so as to open and close the two open ends of the cavity, and the door control baffles and the rat cage rack are provided with multiple vent holes making the cavity and the outside communicated; the adjusting baffle can be inserted in the installation through hole in a pluggable mode, and the cavity can be divided into two secondary cavities in the length direction of the cavity when the adjusting baffle is located in the installation through hole. The rat single-cage fixing device can not be limited by small radiation sources and narrow uniform fields, the radiation dosage precision can be improved to 25.45%, and animals can be conveniently put into the device. The device is successfully used in the biological effect research that high-frequency short-wave radiation causes rat brain injuries.

The present invention relates to an immortalised human neural precursor cell line, NGC-407. The cell line has been established from human foetal tissue. The cell line has been immortalised using a retroviral vector containing the v-myc oncogene. The cell line is a neural progenitor cell line capable of differentiating into to astrocytes and neurons including dopaminergic neurons. NGC-407 cells are capable of migrating to glioblastoma tumours implanted into rat brains and form gap junctions with the tumour cells. NGC-407 cells expressing a suicide gene can be be used for delivering activated prodrugs in the form of activated nucleoside analogs to tumours.

The invention discloses a micro slow positioning injection device for rat brain experiments. The micro slow positioning injection device comprises a support, a needle pushing mechanism and a needle core pushing mechanism; the support is formed by a bottom plate, retractable supporting columns and a top plate, a fixed platform is also slidably arranged on the bottom plate, and a clamping mechanismis slidably arranged on the fixed platform; the needle pushing mechanism comprises a screw rod, two screw sleeves, two connection rods, a first connection plate and a fixation mechanism, wherein the screw rod is fixedly arranged below the top plate, the screw sleeves are symmetrically arranged on the screw rod, the connection rods are hinged to the screw sleeves, the first connection plate is hinged to both the connection rods, and the fixation mechanism is arranged at the middle of the first connection plate and used for fixing a needle cylinder of an injector; the screw rod is composed of two integrated segments, but the spiral directions of screw threads of the two segments are reverse; the needle core pushing mechanism comprises a pushing boosting cylinder which penetrates through thetop plate and is slidably connected with the top plate, a toothed plate which is fixedly arranged at the top of the pushing boosting cylinder, and a gear which meshes with the toothed plate and can conduct self-locking, the gear is slidably arranged on the top plate, and a first handle and scale lines are arranged on the gear. The micro slow positioning injection device effectively avoids experiment errors caused by long-time manual fixation by a tester, and is reasonable in structure and convenient to operate.

The invention discloses novel application of forsythin belonging to active components of natural medicinal plants, and particularly relates to application of forsythin as the only active ingredient to preparation of a medicine for improving a cognitive function and treating Alzheimer's diseases. Animal and cell research results indicate that the forsythin has the exact function of improving the cognitive function and can remarkably improve the spatial learning and memory ability of animals in an aging and Alzheimer's disease model, the level of a center monoamine neurotransmitter of an old-aged animal is increased, the genetic expression of presenilin 2 in a rat brain of an Alzheimer's disease model is reduced, and the survival rate of neuroblastoma cells induced by beta-amyloid protein is increased. The forsythin is matched with relevant auxiliary materials, a health care product or a medicine for purposefully improving the cognitive function and treating the Alzheimer's diseases can be prepared by a conventional preparation method, and the health care product and the medicine can be used for delaying and improving relevant diseases of cognitive hypofunction such as the course of the Alzheimer's diseases, enhancing the healthy quality and the life quality of old people and realizing a healthy aging social value.

The invention relates to a primary culture method of elderly rat brain vascular endothelial cells. The primary culture method comprises the following steps of firstly, taking out a grey matter part of an elderly rat brain tissue, and making a sample tissue; secondly, putting into a culture dish with a separating medium of fetal calf serum, and carrying out blowing, dispersing and biological enzyme digestion so as to prepare dispersed cells; thirdly, centrifuging so as to prepare a blood capillary; fourthly, re-suspending the blood capillary in the separation medium, and digesting to prepare blood capillary suspension; fifthly, centrifuging, re-suspending the sediment in the separation medium, and carrying out Percoll concentration gradient centrifugation so as to prepare the brain vascular endothelial cells; and sixthly, putting in a culture medium, culturing and carrying out digestion passage or freezing and storing. The primary culture method adopts low-concentration pancreatin or PBS (Phosphate Buffer Solution) digestion cells with 1m M EDTA (Ethylene Diamine Tetraacetic Acid), the cells can be subjected to three times of digestion passage but the characteristics of the brain vascular endothelial cells are still maintained, and the purity and the characteristics of the brain vascular endothelial cells are still maintained after being subjected to freezing and recovery, as a result, a great deal of time and money are saved.

The invention discloses a rat brain stereotaxic fitting device. A work table is provided with a first step surface and a second step surface from top to bottom; two upright posts are arranged at the position, along the width, on the first step surface; limit holders are fixedly connected to the positions, at the same height, of the same sides of the two upright posts; each limit holder is providedwith a through hole of which the axis direction parallel to the width of the first step surface; an ear bar is inserted in each through hole in a sliding adjustable manner; the opposite ends of the two ear bars are tip ends. An adjusting mechanism can realize sliding adjustment along the length of the second step surface and is connected with a front tooth rod; the front tooth rod comprises a horizontal plate and a vertical bar; the vertical bar is arranged on the adjusting mechanism and capable of sliding for adjustment along the vertical direction, one end of horizontal plate is fixedly connected to the top end of the vertical bar, the other end of the horizontal plate is provided with a front tooth hole and extends along a gap between the two upright posts. The fitting device is used for fixing rat's head for biological study tests.

The invention provides a bionic positioning method based on rat brain hippocampus space cells. By means of the method, a robot can achieve the positioning function. The method belongs to the field ofbionics. Firstly, a robot explores the space environment, speed information and direction information are collected through a photoelectric encoder, and a speed cell model and a head orientation cellmodel process the linear speed and the angular speed respectively; then, a bidirectional connection network between the grid cells and the position cells is established, on one hand, the grid cells receive excitability input of an upstream cortex, inhibition input between the grid cells and feedback input of the position cells, and on the other hand, projection information of the grid cells drivesthe position cells to discharge; and finally, an environmental expression calculation model of the position cells is constructed, the correlation of the connection strength between the position cellsand the input cells is calculated, the position cells with the highest correlation with the input cells are selected as final winning cells, the discharge information of the cells is associated withthe position points, and the associated information is stored.

Inhibins and activins are protein hormones that reciprocally modulate a diversity of regulatory pathways. Competitive binding experiments revealed that betaglycan, the type III TGF-β receptor, also functions as an inhibin receptor. Betaglycan augments the binding of inhibin to the ActRII activin receptor. By augmenting inhibin binding to ActRII, betaglycan effectively sequesters ActRII away from activin and thereby reduces activin signaling. In addition, the ActRII-betaglycan complex may generate novel signals distinct from those initiated by activin signaling via ActRII and ALK4. Betaglycan is produced in discrete nuclei of the rat brain and by specific cell types within the adult rat pituitary, testis, and ovary. The presence of betaglycan within inhibin-responsive tissues and cell types, together with the ability of this protoglycan to bind inhibin and to confer inhibin sensitivity, is consistent with a role of betaglycan as an inhibin-specific receptor mediating inhibin responses within various tissues.

The invention provides an evaluation model for rat neurotoxicity of parotitis virus, concretely a device for evaluating the neurotoxicity of the parotitis virus. The model comprises(I) a virus inoculation module for performing virus inoculation of the parotitis virus to be evaluated to the lateral ventricle of a rat; (II) a processing module for carrying out vibration slicing on the fixed rat brain; (III) an imaging module for scanning and imaging an obtained mouse brain slice; and (IV) an analysis module used for calculating a neurotoxicity index through a formula I: the neurotoxicity index =S1 / S0 *100 (formula I) according to the cross sectional area S1 of a cavity formed by hydrocephalus in the longitudinal section of the rat brain and the total cross sectional area S0 of the rat brainin the obtained image. The results show that the result is stable, the stability is high, wild strains can be distinguished from vaccine strains, and compared with an existing monkey body neurotoxicity model, the animal cost and the operation difficulty are greatly reduced.

The invention discloses a medicine for preventing and treating brain stroke, especially ischemic stroke and application of the medicine, and the medicine for preventing and treating the brain stroke is ginsenoside Rb1. The invention relates to the medicine for preventing and treating the brain stroke, especially ischemic stroke, belonging to the field of pharmacy. The invention provides the application of the medicine for preventing and treating brain stroke in brain stroke, especially ischemic stroke; ginsenoside Rb1 has a good therapeutic activity in a rat model of brain injury induced by cerebral ischemia-reperfusion, and the experiment proves that ginsenoside Rb1 can obviously relieve the nerve function loss induced by cerebral ischemia-reperfusion in rats, significantly reduce the area of cerebral infarction caused by cerebral ischemia-reperfusion. Inflammatory reaction in the rat brain caused by cerebral ischemia-reperfusion increases the expression of TNF-alpha, IL-1belta and IL-6 proinflammatory cytokines on the focus cerebral side. Ginsenoside Rb1 can reverse the increasing phenomenon of the inflammatory cytokines in the focus cerebral side of the rat and can be used in the prevention and treatment of brain stroke, especially ischemic stroke.

The invention discloses a conduction function detecting system of primary motor cortex nerve in a spinal cord of a rat brain, including stimulation electrodes, a recording electrode, a pulse stimulator and a nerve signal acquisition system, wherein the stimulation electrodes are connected with pulse stimulator, the recording electrode is connected with nerve signal acquisition system, stimulationelectrode adopts tungsten wire single electrode made by MicroProbes Company, USA, electrode type is WE30030.5 A3, axis diameter is 0.081 mm, tip diameter is 2-3[um]m. The recording electrode adopts tungsten wire single electrode manufactured by MicroProbes Company, USA, electrode type is WE30031.0 A3, axis diameter is 0.081 mm, tip diameter is 2-3[um]m. The pulse stimulator uses a Master-9 pulse stimulator with a frequency of 1 Hz and a wave width of 1 ms. As that magnitude of the stimulation current is regulate by the isolator, the invention can detect the conduction path of the nerve signalof the primary motor cortex of the rat brain in the spinal cord, solves the problem of the probe electrode implantation in the microelectronic nerve bridge scheme in the experiment, and provides practical data for the reconstruction of the motor function lost due to the spinal cord injury by the microelectronic technology.