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Micro-fluidic chip for in-vivo on-line simultaneous detection of ascorbic acid and magnesium ion and preparation method thereof

A microfluidic chip and liquid flow technology, which is applied in diagnostic recording/measurement, medical science, instruments, etc., can solve the problems of immaturity and late start of microfluidic chip technology, so as to improve the analysis speed and consume samples and reagent electrode, liquid flow controllable effect

Active Publication Date: 2011-09-14
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But after all, microfluidic chip technology started relatively late and is not yet mature in many aspects. As an emerging cross-technology, its perfection requires the joint efforts of workers in chemistry, microelectronics and micromechanics, materials and life sciences. accomplish

Method used

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  • Micro-fluidic chip for in-vivo on-line simultaneous detection of ascorbic acid and magnesium ion and preparation method thereof
  • Micro-fluidic chip for in-vivo on-line simultaneous detection of ascorbic acid and magnesium ion and preparation method thereof
  • Micro-fluidic chip for in-vivo on-line simultaneous detection of ascorbic acid and magnesium ion and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Preparation of a microfluidic chip capable of simultaneously detecting ascorbic acid and magnesium ions in vivo

[0031] 1) In 6×6cm 2 A "one"-shaped channel is designed in the center of the plexiglass, and its length, width and height are 4cm, 1mm, and 500μm, respectively. A polydimethylsiloxane template with a height of 1 cm was obtained after polydimethylsiloxane was molded twice, and it was cut into a rectangle with a length of 5 cm and a width of 1.5 cm without affecting the use of the channel.

[0032]2) Corrode the indium tin oxide conductive glass with a length of 6 cm and a width of 1.5 cm into two independent centered rectangular base electrodes in the same direction as its length. The electrodes are 1.5 cm and 2.5 cm in length and 0.5 cm in width.

[0033] Firstly, toluidine blue was electropolymerized on a 2.5 cm long substrate electrode, and in a 100 μM toluidine blue solution dissolved in a phosphate buffer solution with a pH of 7, at a scannin...

Embodiment 2

[0038] Example 2. In vitro cross-interference experiment of a microfluidic chip capable of simultaneously detecting ascorbic acid and magnesium ions in vivo

[0039] Under the premise that the liquid flow rate is fixed at 1.0 μL / min, the secondary liquid inflow inlet is continuously fed with β-nicotinamide adenine dinucleotide (abbreviated as NADH) solution (first, NADH is used as a system for detecting magnesium ions. It must exist, and secondly, it has interference in the detection system of ascorbic acid and must be eliminated. Therefore, NADH is a substance that must exist in the downstream detection system but interferes with the upstream detection system and should be excluded. Substances like NADH in the sub-detection can be designed with a secondary liquid flow inlet and sampled from the secondary liquid flow inlet. This can not only ensure the detection of the downstream system, but also eliminate its interference in the upstream system, so as to realize the two-compon...

Embodiment 3

[0044] Example 3. In vitro quantitative detection experiment of a microfluidic chip capable of simultaneously detecting ascorbic acid and magnesium ions in vivo

[0045] Under the premise that the liquid flow rate was fixed at 1.0 μL / min, the NADH solution was continuously fed into the secondary liquid inlet, and the main liquid inlet was mixed with magnesium-free aCSF and different concentrations (mixtures of different concentrations of ascorbic acid and magnesium ions). liquid) between alternate injections.

[0046] image 3 It shows the time-current curves of the microfluidic chip detecting different concentrations of ascorbic acid and magnesium ions in vitro. The black line is the I-t curve of the upstream carbon tube modified working electrode (measurement voltage 0.03V), and the red line is the downstream toluidine blue modification. The I-t curve of the electrode (measurement voltage 0V). The secondary liquid inflow port was fed with 2mM NADH solution continuously, an...

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Abstract

The invention discloses a micro-fluidic chip for in-vivo on-line simultaneous detection of ascorbic acid and magnesium ion and a preparation method and applications thereof. The micro-fluidic chip comprises: a template with a micro channel and a conductive glass substrate which are connected closely; working electrodes which are disposed at positions corresponding to the micro channel on the substrate in a direction of the micro channel, and are arranged in turn with an amount being the same as the amount of components to be detected; a liquid introducing channel and an inlet are disposed at one port of the micro channel on the template, and a liquid extracting channel and an outlet are disposed at the other port; a counter electrode is disposed at the port of the micro channel where the liquid outlet is disposed; on the template, a reference electrode is disposed at a position corresponding to the micro channel between the main liquid introducing channel and the liquid extracting channel. With the micro-fluidic chip, cross interference between ascorbic acid and magnesium ion during the simultaneous detection is eliminated; furthermore, a micro-dialysis in-vivo on-line detection system is combined so as to realize the simultaneous on-line electrochemical detection of ascorbic acid and magnesium ion in rat brains.

Description

technical field [0001] The invention relates to a microfluidic chip capable of simultaneously measuring ascorbic acid and magnesium ions online in a living body and a preparation method thereof. Background technique [0002] Since Manz et al. first proposed the concept of a micro-total analysis system in 1990, microfluidic chip technology has developed rapidly and has become the forefront of the development of the current micro-total analysis system. The microfluidic chip originally originated in the field of analytical chemistry. It is a kind of micro-channel network structure and other functional units made on a substrate of several square centimeters using fine processing technology to realize the collection of micro-sample preparation, sample injection, reaction, A rapid, efficient, and low-cost micro-analysis experimental device that integrates separation and detection. Compared with capillary tubes, microfluidic chips have the advantages of less sample loading, more i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00G01N27/48A61B5/1468
Inventor 毛兰群高霞张自品杨丽芬
Owner INST OF CHEM CHINESE ACAD OF SCI
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