6701 results about "Microfluidic chip" patented technology

Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

A microfluidic device having integrated components for conducting chemical operations. Depending upon the desired application, the components include electrodes for manipulating charged entities, heaters, electrochemical detectors, sensors for temperature, pH, fluid flow, and other useful components. The device may be fabricated from a plastic substrate such as, for example, a substantially saturated norbornene based polymer. The components are integrated into the device by adhering an electrically conductive film to the substrate. The film may be made of metal or an electrically conducting ink and is applied to the device through metal deposition, printing, or other methods for applying films. Methods for reducing bubble formation during electrokinetic separation and methods for heating material in a microfluidic device are also disclosed.

Disclosed are methods, devices and systems for biological and chemical sample processing using microfluidic chips. The disclosed microfluidic chips contain at least two detection zones for interacting with pre-selected RNA sequences, DNA sequences, antibodies, or antigens to determine their presence in the sample. Systems are also described comprising a cassette having at least one port and a sample inlet in fluid communication with a detection zone for interacting with pre-selected RNA sequences, DNA sequences, antibodies, or antigens, or mixtures thereof, if present, in a sample. Methods for concurrent testing of at least two of RNA, DNA, antibody, and antigen in a sample are also described, as are methods for testing for pre-selected pathogens and microfluidic methods.

The invention relates to the fields of analysis and test, in particular to a multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses (SIV) simultaneously. One of the aims of advancing the related diagnosis and treatment technology is to diagnose the SIV quickly and cheaply. The invention provides a diagnosis device, which can fulfill the aims. The key point of the scheme is that: the device, namely the microfluidic chip, comprises pipelines with a parallel structure, wherein the parallel structure comprises three branched pipelines arranged in parallel; three beads-shaped working electrodes are arranged in the three branched pipelines respectively; each beads-shaped working electrode consists of a conductive electrode and a gold colloid sensitive film; the gold colloid sensitive film is attached to the conductive electrode and embeds different subtypes of SIV specific antibodies; the gold colloid sensitive films on the surface layers of the three beads-shaped working electrodes embed three different subtypes of SIV specific antibody substances respectively; and the three different subtypes of SIV specific antibody substances are the subtype H1N1, the subtype H3N2 and the subtype H1N2 SIV specific antibody substances respectively.

Microfluidic systems and components. A microfluidic system includes one or more functional units or microfluidic chips, configured to perform constituent steps in a process and interconnected to form the system. A multi-layer microfluidic system includes a separate dedicated fluid layer and dedicated electromechanical layer connected via through-holes. Electromechanical components are formed on the electromechanical layer.

This invention provides microfluidic devices that comprise a fluidics layer having microfluidic channels and one or more regulating layers that regulate the movement of fluid in the channels. The microfluidic devices can be used to mix one or more fluids. At least a portion of the fluidics layer can be isolated from the regulating layer, for example in the form of a shelf. Such isolated portions can be used as areas in which the temperature of liquids is controlled. Also provided are instruments including thermal control devices into which the microfluidic device is engaged so that the thermal control device controls temperature in the isolated portion, and a movable magnetic assembly including magnets with shields so that a focused magnetic field can be applied to or withdrawn from the isolated portion or any other portion of the microfluidic device. Also provided are methods of mixing fluids. The methods include stacking a plurality of alternating boluses of different liquids in a microfluidic channel, and moving the stacked boluses through the channel. In another method, the boluses are moved into a diaphragm valve having a volume able to accommodate several boluses, and then pumping the liquids out of the valve.

The invention is directed to a method and device for routing, mixing, or reacting droplets or liquid microstreams along the surface of a flat substrate. The flow of liquid microstreams or microdroplets along designated pathways is confined by chemical surface patterning. Individually addressable heating elements, which are embedded in the substrate, can be used to generate flow via thermocapillary effects or to trigger or quench chemical reactions. The open architecture allows the liquid to remain in constant contact with the ambient atmosphere. The device can be used for microfluidic applications or as a surface reactor or biosensor, among other applications.

Disclosed are microflulidic chips that include a plurality of vias; a functionalized porous polymer monolith capable of being in fluid communication with a via; a microarray capable of being in fluid communication with the functionalized porous polymer monolith; and an observation port through which at least one target disposed within the microarray is capable of being detected. The disclosed microfluidic chips contain microarrays that can be effectively coupled to functionalized porous polymer monoliths for capturing and concentrating sample nucleic acids. Also disclosed are microfluidic chips containing microarray probes having observation ports that enable the preparation of microarrays and the detection of targets. These microfluidic chips are capable of capturing and concentrating genetic material for the analysis and identification of biological organisms, such as so-called “threat genes” from chimeric bioweapons.

Microfluidic assay detectors and microfluidic assay detection methods are disclosed. A microfluidic chip is coupled to a light emitting device, emission filters and excitation filters. Excited fluorescent light is detected by a camera and a lens. The correspondent reading allows parallel detection of features such as antigens and biomarkers. A microfluidic filter and related methods are also disclosed. The filter can be used with on-chip fluid filtration such as whole blood filtration for microfluidic blood analysis. The filter is able to filter the necessary volume of fluid and in particular blood in an acceptable time frame.

The invention relates to a special micro-fluidic chip for cholera diagnosis with one-dimensional self-assembly magnetic bead chain electrodes, belonging to the field of testing. Rapid diagnosis of fulminating infectious disease cholera with low cost is one of many expectations in the development of medical technology, and the invention provides a micro-fluidic chip for simultaneously detecting and rapidly diagnosing cholera by using multiple characteristic antibodies of cholera. The chip is provided with three micro liquid storage tanks, and the invention is characterized in that capillary passages in parallel configuration contain in the chip, the parallel configuration contains four micro-passages that are mutually in parallel, four magnetic bead chain working electrodes are respectively installed inside the four micro-passages, the surface substances of the four magnetic bead chain working electrodes are respectively four antibody substances that are cholera TP0821 antibody, cholera TP0319 antibody, cholera TP0624 antibody and cholera O139 mycoprotein antibody. The integrated structure and the appearances of specific magnetic bead chain working electrodes facilitate to improve the diagnosis efficiency of cholera diseases.

This invention provides methods and systems for flushing, washing, and priming microscale devices for reuse. Washing and priming methods include flowing solutions from a manifold to flush wells and microchannels of a microfluidic chip. Systems include manifolds adapted to seal and flow solutions or gasses into chip wells. Devices include microfluidic devices with data storage modules to track the reprocessing status of the microscale devices.

A method for preparing microcapsules using a droplet-based microfluidic chip. Monodisperse microcapsules, which are hollow or can be loaded with a desired material, are prepared using a droplet-based microfluidic chip through the movement of a monomer molecule from the inside of droplets to the interface of droplets, the diffusion of a photoinitiator to the interface of droplets, and the suppression of radical activity by oxygen in droplets. The method involves the use of a simple microfluidic channel and selectively photopolymerizing the shell of the droplets without needing the use of a chemically treated microfluidic channel or a complex microfluidic channel.