Microfluidic devices and methods that use cells such as
cancer cells, stem cells, blood cells for preprocessing, sorting for various biodiagnostics or therapeutical applications are described.
Microfluidics electrical sensing such as measurement of field potential or current and phenomena such as immiscible
fluidics, inertial
fluidics are used as the basis for
cell and molecular
processing (e.g., characterizing, sorting, isolation,
processing, amplification) of different particles, chemical compositions or biospecies (e.g., different cells, cells containing different substances, different particles, different biochemical compositions, proteins, enzymes etc.). Specifically this invention discloses a few sorting schemes for stem cells,
whole blood and circulating
tumor cells and also extracting serum from
whole blood. Further medical diagnostics technology utilizing high
throughput single
cell PCR is described using immiscible
fluidics couple with single or multi cells
trapping technology.