387 results about "3D cell culture" patented technology

Methods for obtaining a protein by culture of hybridoma cells, wherein said protein is an immunoglobulin, are disclosed. The methods involve culturing animal hybridoma cells in continuous presence of an alkanoic acid or salt thereof, which enhances protein production, wherein said alkanoic acid or salt thereof is present at 2 concentration range of 0.1 mM to 200 mM.

Microfluidic devices and methods that use cells such as cancer cells, stem cells, blood cells for preprocessing, sorting for various biodiagnostics or therapeutical applications are described. Microfluidics electrical sensing such as measurement of field potential or current and phenomena such as immiscible fluidics, inertial fluidics are used as the basis for cell and molecular processing (e.g., characterizing, sorting, isolation, processing, amplification) of different particles, chemical compositions or biospecies (e.g., different cells, cells containing different substances, different particles, different biochemical compositions, proteins, enzymes etc.). Specifically this invention discloses a few sorting schemes for stem cells, whole blood and circulating tumor cells and also extracting serum from whole blood. Further medical diagnostics technology utilizing high throughput single cell PCR is described using immiscible fluidics couple with single or multi cells trapping technology.

We introduce a new method for implementing cell-based assays and long-term cell culture. The method is based on digital microfluidics (DMF) which is used to actuate nanoliter droplets of reagents and cells on a planar array of electrodes. DMF method is sutable for assaying and culturing both cells in suspension and cells grown on surface (adherent cells). This method is advantageous for cell culture and assays due to the automated manipulation of multiple reagents in addition to reduced reagent use and analysis time. No adverse effects of actuation by DMF were observed in assays for cell viability, proliferation, and biochemistry. These results suggest that DMF has great potential as a simple yet versatile analytical tool for implementing cell-based assays and cell culture on the microscale.

The embodiments of the invention described herein relate to systems and methods for culturing and / or maintaining intestinal cells, tissues and / or organoids in vitro. The cells, tissues and / or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora.

An apparatus and method for a modular cell culture bioreactor comprises a plurality of chambers for cell culture; at least one reservoir containing a cell support medium; a plurality of conduits fluidly connecting the at least one reservoir with the plurality of chambers; and at least one pump fluidly connected through the plurality of conduits with the at least one reservoir and with the plurality of chambers to pump cell support medium therethrough; wherein each individual chamber of the plurality of chambers includes at least one three-dimensional matrix comprising polyethylene terephthalate, a plurality of channels carrying the cell support medium and having the matrix positioned in fluid communication therebetween, and at least two openings into each the channel, wherein a first the opening is in fluid connection with the pump and a second the opening is in fluid connection with the reservoir.

The present invention provides methods for in vitro production of clinically useful quantities of differentiated human blood cells. In various embodiments of the present invention, immortal pluripotent cells are used to produce differentiated blood cell populations using a cell production device. In a specific embodiment, the device is a sequential series of bioreactors utilizing growth media containing specific combinations of maintenance-, proliferation- or differentiation-promoting factors that maintain, expand and promote the maturation and differentiation of the desired cell types. The immortal pluripotent cells can optionally be genetically modified so as to remove histcompatibility or blood group antigens.

Devices and methods for implementing cell-based assays and long-term cell culture. The device and method are based on digital microfluidics (DMF) which is used to actuate nanoliter droplets of reagents and cells on a planar array of electrodes. DMF method is suitable for assaying and culturing both cells in suspension and cells grown on surface (adherent cells). This method is advantageous for cell culture and assays due to the automated manipulation of multiple reagents in addition to reduced reagent use and analysis time. No adverse effects of actuation by DMF were observed in assays for cell viability, proliferation, and biochemistry. These results suggest that DMF has great potential as a simple yet versatile analytical tool for implementing cell-based assays and cell culture on the microscale.

An improved system for screening a multiple of candidate therapeutic or chemotherapeutic agents for efficacy as to a specific patient, in which a tissue sample from the patient is harvested, cultured and separately exposed to a plurality of treatments and / or therapeutic agents for the purpose of objectively identifying the best treatment or agent for the particular patient. Specific method innovations such as tissue sample preparation techniques render this method practically as well as theoretically useful. One particularly important tissue sample preparation technique is the initial preparation of cohesive multicellular particulates of the tissue sample, rather than enzymatically dissociated cell suspensions or preparations, for initial tissue culture monolayer preparation. With respect to the culturing of malignant cells, for example, it is believed (without any intention of being bound by the theory) that by maintaining the malignant cells within a multicellular particulate of the originating tissue, growth of the malignant cells themselves is facilitated versus the overgrowth of fibroblasts or other cells which tends to occur when suspended tumor cells are grown in culture. Practical monolayers of cells may thus be formed to enable meaningful screening of a plurality of treatments and / or agents. Growth of cells is monitored to ascertain the time to initiate the assay and to determine the growth rate of the cultured cells; sequence and timing of drug addition is also monitored and optimized. By subjecting uniform samples of cells to a wide variety of active agents (and concentrations thereof), the most promising agent and concentration for treatment of a particular patient can be determined. For assays concerning cancer treatment, a two-stage evaluation is contemplated in which both acute cytotoxic and longer term inhibitory effect of a given anti-cancer agent are investigated.

A device for minimizing the formation of bubbles or foam in cell culture is disclosed. The device has a manifold which directs the inflow of cells and cell culture media into a cell culture vessel so as to allow for displaced air or gas to vent from the cell culture vessel without mixing with the incoming cell culture media, thereby preventing the mixing of air and cell culture media and minimizing the formation of bubbles or foam inside the cell culture vessel.

An apparatus and method for a modular cell culture bioreactor comprises a plurality of chambers for cell culture; at least one reservoir containing a cell support medium; a plurality of conduits fluidly connecting the at least one reservoir with the plurality of chambers; and at least one pump fluidly connected through the plurality of conduits with the at least one reservoir and with the plurality of chambers to pump cell support medium therethrough; wherein each individual chamber of the plurality of chambers includes at least one three-dimensional matrix comprising polyethylene terephthalate, a plurality of channels carrying the cell support medium and having the matrix positioned in fluid communication therebetween, and at least two openings into each the channel, wherein a first the opening is in fluid connection with the pump and a second the opening is in fluid connection with the reservoir.

The invention provides a method for preparing a perfusion based 3D cell culture system, a recellularized matrix culture system, and methods of using the culture system.

A well-based flow system for cell culture is described which provides for flow of culture containing compounds for drug screening to be exposed to cells seeded on a membrane. The flow of medium may be planar or radial and means are provided for the removal of waste media through fluid outlets in fluid communication with the assay well plates through conduits. Methods of using the system for cell culture and drug toxicity screening are also provided including coculturing cells such as hepatocytes, stem cells, fibroblasts and smooth muscle cells and selectively exposing cells to test compounds.

A micro device for cell culture is disclosed, which cooperates with a fluid and includes: a top plate having an inlet port; a orifice plate having a plurality of orifices; a culture plate having a plurality of culture wells and a plurality of injection ports; and a bottom plate having at least one collecting well and at least one collecting flow channel, wherein, the culture plate is placed between the orifice plate and the bottom plate. The collecting flow channel connects to all regulating orifices in the culture wells and guides the fluid from the culture wells, then receives the fluid in the collecting well. The fluid flows into the orifice plate from the inlet port of the top plate, and then diversifies into the culture plate, then arrives at each culture well by way of the injection ports, and finally collects in the collecting well of the bottom plate.

A carrier for cell culture comprising an alginic acid gel layer laminated with a gel layer containing a cell adhesion substance, wherein the gel layer containing a cell adhesion substance has a dry thickness of less than 0.3 mum. The carrier for cell culture enables reproducible and convenient cell layer lamination without inhibiting growth and proliferation of cells upon solubilization of the carrier for preparation of a cell sheet.

A device for cell culture capable of automatically performing operations for cell culture over several days to several months while minimizing the risk of contamination. A new medicine can be supplied to an incubator means by using a medicine supply means or unnecessary wastewater can be discharged from the incubator means by using a wastewater discharge means without taking out the incubator means disposed in heat insulation box means from a heat insulation box, and the state of the cell culture can be observed with the incubator means formed in the heat insulation box means. Accordingly, the outside air does not enter directly into the incubator means during culturing, and the risk of contamination is completely eliminated. As a result, the culturing operations can be automatically performed over a long period.

The invention intends to provide a cell culture apparatus which is able to realize an adequate culture according to the culture state of cells while alleviating the labor of an operator. The apparatus includes: a culture bag (18) for causing the cells to proliferate; a cell inoculation cassette (19) (or culture bag (242) as an antibody stimulating and proliferation culture vessel) for stimulating the cells by an inducer for the proliferation; a culture medium cassette (20) for storing a culture medium supplied to the culture bag (18) and the cell inoculation cassette (19); a CCD camera (88) for acquiring images of the cells in the cell inoculation cassette; and an image processing computer and an operation control computer for determining the culture state (proliferation capability and proliferation ability of the cells) of the cells from the images of the cells acquired by the CCD camera, and causing a culture operation to be carried out on the basis of the determination.

The invention relates to a bioreactor (1) for cell culture on a three-dimensional substrate, comprisinga culture chamber (2), the inner walls of which form a vertical duct, preferably, tapered, with a diameter that widens regularly form the duct inlet to the duct outlet, means (3, 4) enabling the culture medium to flow in said vertical duct.The invention also relates to the advantageous use of these bioreactors in tissue engineering, for the production of tissue grafts, notably a bone or cartilage graft.

The automated cell culture arrangement according to the invention comprises at least one closed cell culture module with at least one bioreactor. The closed cell culture module is a closed system, which means that within the closed cell culture module a closed sterile environment can be maintained. The automated cell culture arrangement according to the invention, further comprises at least one pump for pumping liquids within the closed cell culture module and at least one additional tool module, which is configured or configurable to act upon or to monitor the contents of a bioreactor and is movable relative to the at least one closed cell culture module or it is movable relative to one or several components of the at least one closed cell culture module.

Microfluidic devices and methods that use cells such as cancer cells, stem cells, blood cells for preprocessing, sorting for various biodiagnostics or therapeutical applications are described. Microfluidics electrical sensing such as measurement of field potential or current and phenomena such as immiscible fluidics, inertial fluidics are used as the basis for cell and molecular processing (e.g., characterizing, sorting, isolation, processing, amplification) of different particles, chemical compositions or biospecies (e.g., different cells, cells containing different substances, different particles, different biochemical compositions, proteins, enzymes etc.). Specifically this invention discloses a few sorting schemes for stem cells, whole blood and circulating tumor cells and also extracting serum from whole blood. Further medical diagnostics technology utilizing high throughput single cell PCR is described using immiscible fluidics couple with single or multi cells trapping technology.

The invention relates to a device and method for noninvasive continuous monitoring of quantity or concentration of dynamic cells, in particular to a device and method for noninvasive continuous on-line or off-line monitoring of quantity or concentration of dynamic cells in adherent cell cultures (usually as stem cell cultures or other therapeutic cell cultures) or cell and tissue cultures. The device comprises: a cell culture medium supply system, one / one type of or multiple / multiple types of upstream biomedical indicator detection head(s) or connector(s), a cell culture , one / one type of or multiple / multiple types of downstream biomedical indicator detection head(s) or connector(s), one or multiple speed controllable sterile driving unit(s) of fluid, a waste or harvested liquor system, liquid conveying pipeline systems that are driven and controlled in speed by the speed controllable sterile driving unit(s) of fluid for connecting the above components in order. And the upstream and downstream biomedical indicator detection head(s) or connector(s) are connected to a monitoring and controlling system and / or a computer so as to obtain, process and monitor data, and furthermore provide feedbacks for the whole cell culture system through a signal feedback system for realizing control.

A device for cell culture capable of automatically performing operations for cell culture over several days to several months while minimizing the risk of contamination. A new medicine can be supplied to an incubator means by using a medicine supply means or unnecessary wastewater can be discharged from the incubator means by using a wastewater discharge means without taking out the incubator means disposed in heat insulation box means from a heat insulation box, and the state of the cell culture can be observed with the incubator means formed in the heat insulation box means. Accordingly, the outside air does not enter directly into the incubator means during culturing, and the risk of contamination is completely eliminated. As a result, the culturing operations can be automatically performed over a long period.

A self-assembling peptide constituted by D-amino acid is named as d-RAD16 and has an amino acid sequence represented by SEQ ID NO.1 in a sequence list. Test shows that hydrogel formed by the self-assembling peptide can be used for preparing a moisture keeping and water locking agent, and has the function of stopping bleeding rapidly for wound, so that the hydrogel can be used for preparing a hemostatic drug suiting clinical application by adding a pharmaceutically acceptable carrier or excipient. Nanofiber scaffold formed by the self-assembling peptide supports the growth of various cells and can be used as the substrate material for 3D cell culture. The substrate material for 3D cell culture can simulate the in-vivo environment to support the 3D growth of cells in the substrate, thereby providing a cell 3D culture drug screening mode capable of simulating the in-vivo environment and improving the success rate for the development of new drugs.

The systems and methods disclosed herein are generally related to a cell culture system. More particularly, the systems and methods enable the culturing and interconnecting of a plurality of tissue types in a biomimetic environment. By culturing organ specific tissue types within a biomimetic environment and interconnecting each of the organ systems in a physiologically meaningful way, experiments can be conducted on in vitro cells that substantially mimic the responses of in vivo cell populations. In some implementations, the organ systems are fluidically connected with a constant-volume pump.

The present teachings provide a practical cell culture support by which a cell culture with a high degree of freedom can be realized. More specifically, the cell culture support includes a polymer layer exhibiting thermoresponsiveness and a cell culture region obtained by plasma-treating a surface layer portion thereof with a reactive gas, whereby a cell culture support having thermoresponsiveness and cellular adhesiveness while avoiding or limiting the use of cell adhesion factors is provided.

The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.

An automated and robotized platform includes a battery of miniature reactors to contain cell cultures. The platform includes an external sensor to measure an optical property of each cell culture contained in each miniature reactor. The platform also includes a mobile sensor-holder to receive the external sensor. The sensor-holder includes a sensor driving element to move the external sensor from one miniature reactor to another and to measure in real time the at least one optical property. The platform further includes a controlling and processing element to receive real time measurements of the optical property from the external sensor and to provide real time control of a movement of the mobile sensor-holder.