Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cell culture apparatus, cell culture method, cell culture program and cell culture system

A technology of cell culture and immune cell therapy, applied in the direction of tissue cell/virus culture devices, tissue culture, biochemical equipment and methods, etc., can solve the problems of evaluation of cell culture status, complex operations, and inability to reliably grasp cells Issues such as training

Inactive Publication Date: 2008-11-05
迈世耐特股份公司
View PDF2 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, for the culture of suspension cells used in immunocytotherapy, there is no objective evaluation of the culture status of the cells, a device for culturing cells based on the evaluation, etc.
[0009] In addition, in the artificial cell culture including the cell culture system described in Patent Document 2, the operator engaged in the culture has to keep the collection of culture environment data in the constant temperature bath or tank, medium replacement, etc. in the records. Training operations, etc., form a very complicated operation
In addition, there is a possibility that cell culture abnormalities cannot be reliably grasped

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell culture apparatus, cell culture method, cell culture program and cell culture system
  • Cell culture apparatus, cell culture method, cell culture program and cell culture system
  • Cell culture apparatus, cell culture method, cell culture program and cell culture system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0189] Hereinafter, the best mode for carrying out the present invention will be described based on the drawings.

[0190] [A] The first embodiment ( Figure 1 ~ Figure 2 0)

[0191] figure 1 (a) is a block diagram showing the first embodiment of the cell culture device of the present invention, figure 1 (b) is a structural diagram showing the first embodiment of the cell culture system of the present invention. figure 2 yes means figure 1 A perspective view of a constant temperature bath. Figure 5 is shown by figure 2 Layout diagram of the structure of the culture unit consisting of one tank of the constant temperature tank and the culture box accommodated in the tank.

[0192] figure 1 The cell culture device 10 shown in (a) is a device for specially cultivating suspension cells used in immune cell therapy, and has: a constant temperature tank 11 with a plurality of (for example, 8) culture units 12; An operation control panel 13 for the operation of the culture un...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention intends to provide a cell culture apparatus which is able to realize an adequate culture according to the culture state of cells while alleviating the labor of an operator. The apparatus includes: a culture bag (18) for causing the cells to proliferate; a cell inoculation cassette (19) (or culture bag (242) as an antibody stimulating and proliferation culture vessel) for stimulating the cells by an inducer for the proliferation; a culture medium cassette (20) for storing a culture medium supplied to the culture bag (18) and the cell inoculation cassette (19); a CCD camera (88) for acquiring images of the cells in the cell inoculation cassette; and an image processing computer and an operation control computer for determining the culture state (proliferation capability and proliferation ability of the cells) of the cells from the images of the cells acquired by the CCD camera, and causing a culture operation to be carried out on the basis of the determination.

Description

technical field [0001] The present invention relates to a cell culture device, a cell culture method, a cell culture program, and a cell culture system for culturing cells based on evaluation of the culture state of the cells. Background technique [0002] Cell culture, especially cell culture of suspension cells used in immune cell therapy has been basically carried out by manual manipulation. For example, cells collected from a patient are inoculated in a flask to which an antibody (inducing factor) is attached together with a culture medium, and then placed in a thermostat, and the flask is taken out of the thermostat every day, and the culture status is observed using a microscope or the like ( For example, growth status), when growth or the like is observed, or after a predetermined period of time has elapsed from cell inoculation, a medium is added to the above-mentioned flask to culture (for example, proliferate) cells. [0003] In artificial cell culture as describe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/06C12N5/02
CPCC12M41/46C12M23/14C12M41/36
Inventor 幡多德彦神宫司英雅野口敦崇佐藤志保佐藤和聪有吉直子细井亮宏
Owner 迈世耐特股份公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products