Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously

Abstract

The invention relates to the fields of analysis and test, in particular to a multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses (SIV) simultaneously. One of the aims of advancing the related diagnosis and treatment technology is to diagnose the SIV quickly and cheaply. The invention provides a diagnosis device, which can fulfill the aims. The key point of the scheme is that: the device, namely the microfluidic chip, comprises pipelines with a parallel structure, wherein the parallel structure comprises three branched pipelines arranged in parallel; three beads-shaped working electrodes are arranged in the three branched pipelines respectively; each beads-shaped working electrode consists of a conductive electrode and a gold colloid sensitive film; the gold colloid sensitive film is attached to the conductive electrode and embeds different subtypes of SIV specific antibodies; the gold colloid sensitive films on the surface layers of the three beads-shaped working electrodes embed three different subtypes of SIV specific antibody substances respectively; and the three different subtypes of SIV specific antibody substances are the subtype H1N1, the subtype H3N2 and the subtype H1N2 SIV specific antibody substances respectively.

Description

technical field [0001] The invention relates to a multi-channel microfluidic chip for simultaneous detection of multiple subtypes of swine influenza virus. The swine influenza virus diagnostic device is a special microfluidic chip for diagnosing swine influenza virus antigens based on antigen / antibody specific reactions, belonging to Analyze test areas. Background technique [0002] Swine influenza virus (Swine influenza vires, SIV) belongs to Orthomyxoviridae Influenza virus A (Influenza virus A), is a single-stranded negative-sense RNA virus, and its genome consists of 8 independent segments of varying sizes. The subtypes of influenza A are classified according to the difference of hemagglutinin and neuraminidase, which can be divided into 15 different H antigen subtypes and 9 different N antigen subtypes. At present, there are three main subtypes of SIV serotypes that are popular in the world: H 1 N 1 、H 3 N 2 and H 1 N 2 . Recent global outbreaks of influenza A H...

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Problems solved by technology

(4) At present, microfluidic sensors are mostly based on optical detection systems. The instruments are expensive and bulky, and full integration is difficult. Moreover, the detection cost is high, which limits its promotion.

[0005] At present, in the field of microfluidic chip technology, the related technologies and methods of using specific antibodies of multiple subtypes of swine influenza virus (SIV) to simultaneously detect and rapidly diagnose multiple subtypes of SIV antigens have not been reported.

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