The invention discloses a hexagonal surface wave acoustic tweezer chip for cell arrangement and assembly. The hexagonal surface wave acoustic tweezer chip includes a hexagonal acoustic tweezer and a microfluidic cavity, a Z-cut lithium niobate piezoelectric substrate is used, six interdigital transducers are made on the substrate, through the combination of different beams and individual phase modulation of the beams, sound field patterns far more than traditional acoustic tweezers can be produced, a sound field structure is also more flexible and adjustable, and when the chip is used for cellmanipulation and assembly, a more diverse assembly structure and more powerful control ability are provided. Through the individual modulation and combination application of the transducers, multi-wave interference can be carried out in the way, a variety of sound field structures and flexible regulation are realized. The hexagonal surface wave acoustic tweezer chip can be more suitable for various application scenarios that require cell manipulation and assembly without damage and contact, a more flexible surface wave sound field pattern is realized, the hexagonal surface wave acoustic tweezer chip is more suitable for various cell manipulation needs of biological research and tissue engineering, and the application prospects are huge.