93 results about "Cells isolation" patented technology

The present technology relates to protocols relative to utility meters associated with an open operational framework. More particularly, the present subject matter relates to protocol subject matter for advanced metering infrastructure, adaptable to various international standards, while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field. The present subject matter supports meters within an ANSI standard C12.22/C12.19 system while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field, all to permit cell-based adaptive insertion of C12.22 meters within an open framework. Cell isolation is provided through quasi-orthogonal sequences in a frequency hopping network. Additional features relate to Outage notification system features, and corresponding methodology and apparatus subject matters, both at the network and device level.

The present technology relates to protocols relative to utility meters associated with an open operational framework. More particularly, the present subject matter relates to protocol subject matter for advanced metering infrastructure, adaptable to various international standards, while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field. The present subject matter supports meters within an ANSI standard C12.22/C12.19 system while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field, all to permit cell-based adaptive insertion of C12.22 meters within an open framework. Cell isolation is provided through quasi-orthogonal sequences in a frequency hopping network. Additional features relate to apparatus and methodology subject matters relating to broadcast acknowledgement features.

Systems and methods for generating a high voltage pulse. A series of voltage cells are connected such that charging capacitors can be charged in parallel and discharged in series. Each cell includes a main switch and a return switch. When the main switches are turned on, the capacitors in the cells are in series and discharge. When the main switches are turned off and the return switches are turned on, the capacitors charge in parallel. One or more of the cells can be inactive without preventing a pulse from being generated. The amplitude, duration, rise time, and fall time can be controlled with the voltage cells. Each voltage cell may also include a balance network to match the stray capacitance seen by each voltage cell. Droop compensation is also enabled. Isolation diodes ensure that a discharge current can bypass inoperable voltage cells.

Embodiments of the present invention provide methods for fabricating a solar cell on a substrate that have proportionally reduced current to minimize or reduce the likelihood of shading of a portion of the solar cell causing damage to the formed device. In one embodiment, a method for fabricating a series of solar cell arrays on a substrate includes providing a substrate having a TCO layer formed thereon, forming a first plurality of vertical scribing lines and a first plurality of horizontal scribing lines in the TCO layer, forming a film stack and a back metal layer on the scribed TCO layer, and forming a second plurality of the horizontal scribing lines in the film stack and the back metal layer, wherein the second plurality of horizontal scribing lines comprise pairs of scribing lines formed adjacent to each respective one of the first plurality of the horizontal scribing lines formed in the TCO layer.

The present invention is apllied in fields like biological medicine and tissue engineering. A fluid control system in a cell isolation and culture system is used to automatically process sample preparation, circulating tumor cell (CTC) isolation, plate changing and cell culturing. By using the present invention, time and labor are saved; moreover, the present invention has a small size and is easily carried.

The invention discloses a cell-culture micro-fluidic chip with a self-antibacterial function. The cell-culture micro-fluidic chip comprises a flexible polymer surface-layer thin film, an upper-layer chip, a lower-layer chip, a micro valve and a guide pipe, wherein the flexible polymer surface-layer thin film is provided with a cell culture chamber, a culture-solution guide-pipe through hole, a waste-culture-solution micro-valve through hole, a buffer-solution guide-pipe through hole and an enrichment-cavity micro-valve through hole; the upper-layer chip is provided with a cell-culture-chamber bottom groove, a waste-culture-solution micro-valve bottom groove, a waste-culture-solution outlet, a buffer-solution guide-pipe bottom groove, a cell-separation flow channel, an enrichment cavity, an enrichment-cavity micro-valve bottom groove and a waste-separation-solution outlet; the upper surface of the lower-layer chip is paved with driving electrodes corresponding to the positions of the cell-culture-chamber bottom groove, the cell-separation flow channel and the enrichment cavity; the guide pipe comprises a culture-solution guide pipe and a buffer-solution guide pipe; and the micro valve comprises a waste-culture-solution micro valve and an enrichment-cavity micro valve. The cell-culture micro-fluidic chip disclosed by the invention has the advantages of minimization, high efficiency, low cost, repeatable use and self-antibacterial function and the like, and has large use prospect in the biomedicine.

The embodiment of the invention provides a micro-fluidic chip for isolating and capturing a single cell. The micro-fluidic chip comprises a chip substrate; the chip substrate is provided with a sampleinjection hole and a plurality of sampling holes; the sampling injection hole is located in the center of the chip substrate, wherein the sample injection hole communicates with at least two groups of multistage flow channels, each group of multistage flow channel comprises a main flow channel, a first branch flow channel and a plurality of second branch flow channels; one end of the main flow channel communicates with the sample injection hole, and the other end of the main flow channel communicates with the first branch flow channel; and the plurality of second branch flow channel are symmetrically arranged at two sides of the first branch flow channel, one end of each of the plurality of second branch flow channels communicates with the first branch flow channel, the other end of eachof the second branch flow channels is connected with at least one of the sampling holes, and each of the sampling holes communicates with one of the second branch flow channels. By using the micro-fluidic chip, the single chip can be rapidly and simply isolated and captured with low cost and low threshold. The invention further provides a preparation method and application of the micro-fluidic chip.

A method for analyzing single cells by mass spectrometry includes the steps of providing a plurality of cells in a liquid medium and placing the cells and liquid medium in a single cell isolation and ejection system. Liquid medium containing a single cell is released from the single cell isolation and ejection system. The liquid medium and single cell are captured in a capture probe containing a flowing capture probe solvent. The cell is lysed by a lysis inducer in the capture probe to disperse single cell components into the medium. The lysed single cell components are transported to a mass spectrometer, where the lysed single cell components entering the mass spectrometer are spatially and temporally separated from any dispersed components of another single cell from the sample entering the mass spectrometer. Mass spectrometry is conducted on the lysed single-cell components. A system for analyzing single cells by mass spectrometry is also disclosed.

The invention discloses a microfluidic chip for accurately controlling and pairing single particles. The chip comprises a channel layer and a control layer. The channel layer comprises a plurality ofunits for capturing and transferring single particles, and each unit consists of a capture flow channel, a capture chamber, a capture gap, a transfer flow channel, a pairing chamber and a pairing gap.The control layer is positioned below the capture flow channel and a pairing flow channel, is vertical to the capture flow channel and the pairing flow channel, and is isolated by a diaphragm. The chip can efficiently and accurately control the capture and transfer of single particles, and can realize high-throughput and high-efficiency single-particle pairing after different rounds of single-particle capture and transfer, and the number and the type of the paired particles are controllable. The chip can be widely applied to single-cell isolation and culture, single-cell heterogeneity analysis, multi-cell co-culture, multi-cell interaction, potential mechanism disclosure and the like.