The invention discloses a micro flow control chip used for multi-index biochemical detection. The micro flow control chip comprises a micro-fluid channel, a plurality of reaction tanks and a pneumatic micro-valve, wherein one opening end of the micro-fluid channel is a sample inlet while the other opening end is communicated with a chamber arranged in the micro flow control chip; the top wall of the chamber is an elastic film and is exposed in air; reaction tanks are connected in series or in parallel through the micro-fluid channel; the pneumatic micro-valve comprises a control channel; one sidewall of the control channel is an elastic film; and the control channel and the micro-fluid channel are arranged crossly. The micro flow control chip used for multi-index biochemical detection integrates sample introduction with multi-index biochemical reaction based on a micro flow control chip technical platform, so that the micro flow control chip used for multi-index biochemical detection, disclosed by the invention, has the advantages of greatly simplifying operating process, reducing consumption of samples and reagents, having no need to allocate expensive instruments, greatly reducing use cost, making on-site instant detection possible and having great foreseeable economic value and social value.