618 results about "Biochemical detection" patented technology

A detecting device for biochemical detections is provided. The detecting device includes a first substrate, a magnetic layer located on the first substrate, an isolation layer located on the magnetic layer, at least a first electrode located on the isolation layer, a first dielectric layer located on the first electrode, a first hydrophobic layer located on the first dielectric layer, a second substrate, at least a second electrode located on the second substrate and having a cathode and an anode, a second dielectric layer located on the second electrode and a second hydrophobic layer located on the second dielectric layer. The first electrode is zigzag-shaped, and the cathode and the anode of the second electrode are comb-shaped and interlaced with each other.

The invention provides a medical laboratory clinical biochemical detection automatic checking method and system. The method employs a computer software and hardware system and programs multiple checking rules to form a multiple checking rule execution module to automatically check the detection results of various biochemical immunity detection items. The detection results of the various biochemical immunity detection items cannot be sent to an automatic checking unit for automatic checking until they pass the multiple checking rules; if the detection results of the various biochemical immunity detection items cannot pass the above-mentioned checking rules, the detection results are transferred to a manual checking unit for checking; only after the detection results are checked by the manual checking unit or checked again after automatic treatment of dilution, reexamination, test item adding, unqualified sample return and the like by an assembly line can a detection report be issued; the multiple checking rules mainly include a clinical information judging rule, a sample state judging rule, an indoor quality control judging rule, an instrument state judging rule, a range judging rule, a difference judging rule, and a logical relationship judging rule. The checking rules are reasonable in design and cover the pre-analysis, analysis and post-analysis processes, thereby realizing whole-process quality control on a detection analysis process and guaranteeing the accuracy of detection results.

The invention discloses a micro flow control chip used for multi-index biochemical detection. The micro flow control chip comprises a micro-fluid channel, a plurality of reaction tanks and a pneumatic micro-valve, wherein one opening end of the micro-fluid channel is a sample inlet while the other opening end is communicated with a chamber arranged in the micro flow control chip; the top wall of the chamber is an elastic film and is exposed in air; reaction tanks are connected in series or in parallel through the micro-fluid channel; the pneumatic micro-valve comprises a control channel; one sidewall of the control channel is an elastic film; and the control channel and the micro-fluid channel are arranged crossly. The micro flow control chip used for multi-index biochemical detection integrates sample introduction with multi-index biochemical reaction based on a micro flow control chip technical platform, so that the micro flow control chip used for multi-index biochemical detection, disclosed by the invention, has the advantages of greatly simplifying operating process, reducing consumption of samples and reagents, having no need to allocate expensive instruments, greatly reducing use cost, making on-site instant detection possible and having great foreseeable economic value and social value.

Various embodiments provide devices, methods, and systems for high throughput biomolecule detection using transducer arrays. In one embodiment, a transducer array made up of transducer elements may be used to detect byproducts from chemical reactions that involve redox genic tags. Each transducer element may include at least a reaction chamber and a fingerprinting region, configured to flow a fluid from the reaction chamber through the fingerprinting region. The reaction chamber can include a molecule attachment region and the fingerprinting region can include at least one set of electrodes separated by a nanogap for conducting redox cycling reactions. In embodiments, by flowing the chamber content obtained from a reaction of a latent redox tagged probe molecule, a catalyst, and a target molecule in the reaction chamber through the fingerprinting region, the redox cycling reactions can be detected to identify redox-tagged biomolecules.

The present invention provides for stable nicotinamide adenine dinucleotide (NAD / NADH) and nicotinamide adenine dinucleotide phosphate (NADP / NADPH) derivatives of formula (I), enzyme complexes of these derivatives and their use in biochemical detection methods and reagent kits.

The invention provides an optical fiber F-P multifunctional sensor based on a microporous optical reflection diaphragm. The optical fiber F-P multifunctional sensor based on the microporous optical reflection diaphragm is characterized in that the design of an F-P cavity structure formed by sequentially connecting a single-die optical fiber, an optical capillary tube and the microporous optical reflection diaphragm and a special microporous optical reflection diaphragm structure is adopted to develop a built-in optical fiber F-P multifunctional sensor which can highly sensitively detecting liquid refractive index, air humidity and ultrasound under common air pressure and external high pressure conditions. The sensor has a micro size, is simple in structure, integrated, high in sensitivity, fast to respond, high in resistance to electromagnetic interference, applicable to the fields of environmental monitoring, and biochemical detection and ultrasonic detection, has a good market prospect, and is high in market value.

The present invention relates to magnetic particle separators using micromachined magnetic arrays and more particularly, to magnetic particle separators or manipulators using controlled magnetization on micromachined magnetic arrays for the separation of cells and other biological materials. The present invention also pertains to using such devices for the separation and analysis of biological materials for immunoassays, DNA sequencing, protein analysis, and biochemical detection applications. The present invention can also be viewed as a novel method for fabricating fully integrated permanent magnet components within any microelectromechanical system (“MEMS”) structures. The present invention also provides a magnetic particle separation and manipulation system for rapid separation and accurate manipulation of magnetic particles in two-dimensional electromagnetic arrays, which utilize high throughput biological analyses. A disposable cartridge can be produced in low cost using a low cost substrate such as plastic or other polymer, glass, or metal. Magnetic flux is generated by conventional or micromachined electromagnets a platform system consisting of magnetic flux sources, magnetic flux guidance, and a microprocessor control interface. By controlling direction of electric currents into inductors on the platform system, arbitrary magnetic poles can be generated on Permalloy structures of the cartridge to separate and manipulate magnetic particles. The magnetic particle separator and manipulator in the present invention can be easily combined with automated detection systems such as a fluorescent monitoring system.

The invention relates to a preparation method of a crack-free large-area optical crystal, in particular to a preparation method of a crack-free large-area opal structure and an inverse opal structural optical crystal. A support substrate of the optical crystal selected in the invention is a hydrophilic flexible substrate, and the crack-free large-area opal structure or a composite opal structural optical crystal can be obtained by performing self-assembly on monodispersed colloid particles on the support substrate or performing co-assembly and polymerization on the monodispersed colloid particles and a water-soluble polymer monomer on the hydrophilic flexible substrate. A crack-free large-area inverse opal structural optical crystal can be obtained by further dissolving the monodispersed colloid particles in the composite opal structural optical crystal. The preparation method related to the invention has the advantages of low cost, easiness for operating, environmental friendliness and high generality. The prepared optical crystal can be practically applied to the fields of high-performance optical devices, chemistry, biochemical detection, and the like.

The invention belongs to the technical field of the preparation methods of the nanomaterials and specifically discloses a method for preparing water-soluble fluorescent silicon quantum dots by using a hydrothermal process. The method comprises the steps of firstly introducing an amino silane and a reducing agent into a solvent in which nitrogen is introduced and mixing, continuing introducing the nitrogen for protection for a while, next, transferring to a hydrothermal reaction kettle, and heating for reacting for a while, thereby obtaining the fluorescent silicon quantum dots having excellent chemical properties. The method has the advantages of low raw material cost due to the adoption of the amino silane as a silicon source, simple operation steps, direction synthesis in water phase, easy large-scale production, and being green and environment; the obtained quantum dots have high quantum yield and excellent chemical characteristics, and are small in particle size, evenly in distribution, non-toxic, good in biocompatibility and acid resistance; besides, the quantum dots have pH sensitive characteristic in a certain range, and can be widely applied to the biochemical and biomedical sensing fields such as biochemical detection, drug analysis, cell and living imaging and targeting tracing and also can be used as photovoltaic conversion and light-emitting display materials.

The invention relates to an angle adjustable multichannel optical fiber surface plasmon resonance sensing probe and belongs to the technical field of biochemical detection and optical fiber sensing. The angle adjustable multichannel optical fiber surface plasmon resonance sensing probe is characterized by consisting of surface plasmon resonance (SPR) sensing surfaces and a reflective surface; the opposite SPR sensing surfaces and the reflective surface form an included angle of 90 degrees, and form a sensing channel; a Cr membrane with the thickness of between 2 and 7 nanometers is adhered to an optical fiber core tightly and is plated on each SPR sensing surface, and an Au membrane or an Ag membrane which is 35 to 65 nanometers thick is adhered to the Cr membrane tightly and is plated on each SPR sensing surface; the Au membrane or the Ag membrane is plated on the reflective surface to form a micro-reflector; and an angle of each SPR sensing surface relative to an optical fiber axis is adjustable, a plurality of groups of SPR sensing surfaces which are complementary mutually and the reflective surface concentrate at one end of the same optical fiber and meet at a point to form a multi-surface pyramid structure, and a plurality of groups of SPR sensing surfaces generate surface plasmon resonance sensing simultaneously. The angle adjustable multichannel optical fiber surface plasmon resonance sensing probe has the advantages that: incidence angles in the optical fibers can be controlled to realize the portable and parallel detection of multichannel multi-parameter; and the application value is high.

The present invention discloses a conformal light-sensing structure integrated digital microfluidic chip which can monitor a chemical reaction between droplets carrying different components in a digital microfluidic chip in real-time, in the detection application field of digital microfluidic biochemical detection on consumption for a low level sample. According to the chip disclosed by the present invention, on the basis of no change of a current structure and property of electrowetting, a subwavelength scale optical sensing structure is embedded between electrowetting adjacent electrode gaps; a co-planar conformal structure with a hydrophobic layer of the electrowetting structure is kept in structure; by detecting a change of a light-sensing structure light field which is caused by a small change in a refractive index occurring during a process of the droplets reaction, determination for composition and location of a microdroplet is realized. Compared with light sensing composed of a conventional capacitor, an electrical impedance sensor, an electrochemical sensor and a discrete element, the chip disclosed by the present invention has the advantages of being simple in structure, standard CMOS compatible process which is easier to integrate, low in cost and high in accuracy. In addition, a characteristic that a sensing structure is conformal with an electrowetting structure enables a sensing function to be added without reducing the electrowetting performance.

A biochemical detecting device for separating a reagent, a plurality of magnetic beads and a target from a mixture, and detecting the target is provided. The biochemical detecting device includes a first substrate, at least two first electrode sets located on the first substrate, a second electrode set located on the first substrate and between the two first electrode sets, and a second substrate covering the first substrate, each of the first electrode sets and the second electrode set. Accordingly, the movement of the mixture is digitally controlled by the provided biological detecting device.

The invention provides an apparatus and methodology to carry out biochemical testing on a centrifugal platform using flow splitting technique. In conventional biochemical testing, reagents need to be loaded individually into each reservoir. By using the flow splitting technique in this invention, one reagent only need to be loaded once, then, it can be evenly distributed into each reaction chambers in single or multiple layers format. The invention greatly reduces the required manpower when large numbers of assays are integrated on one platform. Because of the invention, many medical examinations can be performed efficiently, thus reduce the waste of manpower, time and cost.

A liquid micro drop control chip used in virus detection consists of top and bottom substrates as well as support structure. It is featured as preparing said substrates and said structure by micromechanical means; forming bottom substrate by bottom base plate, electrode array and insulated hydrophobic layer; forming top substrate by top base plate and hydrophobic layer; setting liquid channel, liquid storage region and detection region between top and bottom substrates.

The invention discloses an intelligent water closet with automatic display of urine test information, comprising a water closet main body, an intelligent urine detecting unit for biochemical urine detection and an intelligent urine biological information database management system. The intelligent urine detecting unit is arranged in the water closet main body and is connected with the intelligent urine biological information database management system which is used for judging the health condition of a person by comparing data obtained after biochemical urine detection of the intelligent urine detecting unit with the standard data. By adopting the intelligent water closet with automatic display of urine test information, biochemical detection can be carried out on urine each time so as to lead people to learn about the health condition at any time and carry out prevention and treatment according to the detection result; in addition, the water closet can also distinguish whether metabolic biological information is in balance, in negative balance or out of balance, so as to lead people to timely regulate diets and live a healthy life every day.

A miniature biochemical analyzer using dual-spectrum detection is composed of the first optical channel consisting of adaptive regulatable light source, optical attenuator, the first specimen chamber, the first integrated spectrometer and the first A / D converter, the second optical channel consisting to the second specimen chamber, the second integrated spectrometer and the second A / D converter, and the computer data processing system. Its advantages include wide dynamic range and more functions of adaptive dynamic regulation, self scaling, and real-time error correction.

The invention relates to a sample detecting system of a micro biochemical detection instrument. The sample detecting system comprises an optical system, a spectrum scanning system and a temperature control system, wherein the detecting system is controlled by an embedded type ARM9S3C2410 in real time, so that the optical system, the spectrum scanning system and the temperature control system coordinately cooperate to rapidly detect biochemical indexes in real time. The sample detecting system can realize pulse positioning and precise and random positioning of various sample chambers, has the characteristics of simple structure, small size, convenience to carry, low power consumption and the like, and is widely applied to national defense, after-calamity emergency treatment, biochemistry, clinical medicine, environment science and the like.

The invention relates to a multiparameter immunity chromatographic test strip quantitive detection instrument, which relates to the biochemical detection technology, adopts optical fibers as optical transmission media, simultaneously detects optical signals of a detection line and a quality control line through a CCD, analyzes the absorptivity, and obtains the concentration of an object to be detected in sample solution. The invention comprises a photoelectric detection module, a signal acquisition processing module, a control and operation module, a temperature compensation module and so on. The multiparameter immunity chromatographic test strip quantitive detection instrument adopts the method of simultaneously measuring the detection signals and the quality control signals to eliminate the interference caused by the external environment in order to improve the stability and the sensibility of detection; the photoelectric detection module adopts the optical fibers as the transmission media and the CCD as a photoelectric detecting element on the basis of the light reflection principle, and can perform quantitive measurement on a multiplex immunity chromatographic test strip; simultaneously, due to great affection of the ambient temperature on immunoreaction, the temperature compensation module is arranged; the quantitive detection instrument increases a parameter set item on the software design and can set related parameters aiming at the immunity chromatographic test strip to be detected so as to realize measurement of different parameters.

The invention provides a method for simultaneously detecting 13 kinds of steroid hormones in serum. The method includes the following steps: a to-be-tested serum sample is mixed with an internal standard solution, a detection liquid is obtained by liquid-liquid extraction, and detection is performed by ultra-high-performance liquid chromatography-tandem mass spectrometry; liquid chromatography conditions are that: a mobile phase A is a formic acid aqueous solution of 0.1%, and a mobile phase B is a formic acid methanol solution of 0.1%; mass spectrometry conditions are that: a positive ion mode employs a multi-reaction monitoring mass spectrometry scanning mode; and the 13 kinds of steroid hormones include pregnenolone, progesterone, 17-hydroxypregnenolone, 17[alpha]-hydroxyprogesterone, 11[alpha]-hydroxyprogesterone, 21-deoxycortisol, 11-deoxycorticosterone, corticosterone, cortisol, 11-deoxycortisol, cortisone, aldosterone, and estrone. The method simultaneously detects the 13 kindsof steroid hormones with the detection time of only 6 minutes, and is easy to operate and takes less time; the sensitivity is high, the accuracy is good, the specificity is strong, a detection range is wide, and an application prospect in medical and biochemical detection fields is broad.

The invention discloses a microstructural resonant sensor based on micro-electro-machinery, which is characterized in that two micro torsional pendulum resonators are arranged side by side; a sensitive layer film for detection is arranged on one micro torsional pendulum resonator; a non-sensitive layer film for difference detection is arranged on the other micro torsional pendulum resonator; each micro torsional pendulum resonator consists of a pendulum shaft and pendulum plates distributed on the two sides of the pendulum shaft; an electromagnetic drive coil and a vibration pickup coil are arranged on the pendulum plates. When being electrified, the drive coil under the action of lorentz force of an external magnetic field generates a torque around the pendulum shaft to drive the torsional pendulum resonators to vibrate; vibrating signals are picked up by an induced electromotive force generated by the vibration pickup coil by cutting the lines of magnetic force, and fed back to the drive coil to form closed-loop self-excitation and output a resonant frequency. The microstructural resonant sensor operates under a torsion mode and increases the merit factor; the difference output inhibits errors caused by the effect of external elements such as temperature; the microstructural resonant sensor has simple process and high detection sensitivity, and can be used as a biochemical sensor for detecting micro biochemical molecules, particularly for biochemical detection and sensing under liquidoid conditions.

The invention discloses a skin condition quantitative evaluation method based on a gray level co-occurrence matrix. The method mainly comprises the following steps: reading a human skin image acquired by a high-definition camera instrument; calculating the average gray level of the skin image; correcting hair pixels; calculating a gray level co-occurrence matrix; calculating the feature parameters of the skin image according to the gray level co-occurrence matrix, including angular second moment, contrast, entropy and correlation; calculating the comprehensive index of skin condition; and finally, comprehensively evaluating the skin image according to the calculation result. The gray level co-occurrence matrix based on the skin image and the feature parameters of the skin image can truly and objectively reflect the condition and physical properties of skin. The comprehensive index can be used to quantitatively analyze and evaluate the skin texture. The evaluation result is basically the same with the trend of effect based on biochemical detection. A quantitative, fast, scientific and convenient evaluation method is realized. The method can be used to quantitatively analyze and evaluate the state of skin.

The present invention relates to an immunological and biochemical detection system, which includes a mobile terminal, an immunological and biochemical detector and a cloud server; the mobile terminal is provided with a scan code module, a first main control module, a first wireless communication module and a display module; the immunological and biochemical detector includes a second main control module, a second wireless communication module, a detection module, a test paper box and an image acquisition module, a two-dimensional code is arranged on the test paper box; the mobile terminal scans the two-dimensional code on the test paper box to get test paper information, and sends a corresponding biochemical substance detection control signal to the immunological and biochemical detector; the immunological and biochemical detector tests a sample on test paper in the test paper box, and sends detected test paper image information to the cloud server; and the cloud server analyzes the image information to obtain the content of a biochemical substance, and sends, by network, the analyzed content of the biochemical substance to the mobile terminal for displaying. The beneficial effects are that: the immunological and biochemical detection system has the ability to intelligently network, and is highly integrated and easy to use.

The invention discloses a multifunctional medical detector which comprises a sample adding mechanism, an optical detection mechanism, a reaction bin, a driving mechanism, an electrode tip and a detection result processing module, wherein the driving mechanism is provided with a driving output shaft, and the driving output shaft is located in the middle of the reaction bin and used for driving a reagent plate in the reaction bin to rotate; the optical detection mechanism is located below the reaction bin, sequentially projecting light rays to at least one dry reagent pieces, used for biochemical detection, of the reagent plate along with rotation of the reagent plate, and receiving the light rays reflected by the at least one dry reagent piece to obtain at least one reflection response voltage value for biochemical detection; the electrode tip is located at the upper part of the reaction bin, provided with at least two telescopic electrode contacts, used for sequentially contacting the electrode on the reagent plate through the electrode contacts along with rotation of the reagent plate to read biochemical detection and / or blood routine examination voltage detection values of samples. The invention further discloses a multifunctional medical detection system.

The invention belongs to the field of crop pesticide residue detection and biochemical detection, and discloses an automatic portable paper-based micro-fluidic photoelectric detection device and method of pesticide residue. A paper-based chip carrying an indophenol acetate reagent in a first test paper box is conveyed by a chip replacing and conveying belt to a chip installing strip, a paper-based chip carrying an acetylcholin esterase reagent in a second test paper box is conveyed by a chip outgoing and conveying belt to a lower installing strip of an acetylcholin esterase paper-based micro-fluidic chip; a reagent enters a sampling tank through a sampling port, enters an acetylcholin esterase fixed groove under the effect of a paper-based chip sampling channel and is mixed with the acetylcholin esterase; the chip installing strip is driven to move downwards by pressing a component, so that the indophenol acetate reagent and the acetylcholin esterase reagent are in full contact with each other and generate color reaction, and a color region is detected by a photoelectric detection device. The automatic portable paper-based micro-fluidic photoelectric detection device and method are based on an enzyme inhibition reaction principle, obviously improve the detection resolution and sensitivity, and realize an automatic process from inputting a sample to be detected to displaying the amount of pesticide residue.

The invention discloses a protein covalent coupling method on the surface of magnetic beads. The method comprises the steps that: (1) the magnetic beads and an activating agent are subjected to co-incubation, such that activated magnetic beads are obtained; (2) the activated beads are subjected to co-incubation with protein, such that magnetic beads coupled with the protein are obtained; (3) the magnetic beads coupled with the protein are treated by using a blocking solution, such that surface active sites are blocked; and (4) the magnetic beads obtained in the step (3) are subjected to simultaneous vibration and sieving, and the sieved magnetic beads are collected. The method provided by the invention has the advantages of high protein coupling efficiency, good repeatability, and versatility. With the method, biological activities of protein coupled on the magnetic beads are not influenced. With the method provided by the invention, the obtained magnetic beads with covalently coupled protein can be used in fields such as immunoassay, biochemical detection, molecular detection, cell typing, and the like.

The invention discloses a dual-detection biochemical sensing detector integrated with optofluidics. The dual-detection biochemical sensing detector comprises a light source system, a light path reshaping system, a sensing chip, a three-dimensional control system, a surface-enhanced Raman SERS (Surface Enhanced Raman Scattering) detection system, a local surface plasma resonance LSPR (Localised Surface Plasmon Resonance) detection system and an automatic sampling control system. According to the sensing chip disclosed by the invention, two optical detection means of the LSPR and SERS are simultaneously adopted and are combined with microfluidics to form a novel microfluidics optical sensing chip; the application of traditional LSPR or SERS is extended by the technology; the sensing chip based on a double plasma structure has great attraction for biochemistry detection based on a liquid sample; and the research and application of the microfluidics optical sensing system are accelerated. The sample is detected by using two complementary modes of the LSPR and SERS; and the combination of the LSPR and SERS analytic technologies on the same substrate is realized.

The invention provides a seminal plasma compound quality control product which comprises freeze-dried powder prepared by mixing a quality control preserving fluid and a seminal plasma stock solution, wherein the quality control preserving fluid is a water solution containing 0.47-0.67 percent of sugar, 0.07-0.17 percent of salt and 0.63-0.83 percent of enzyme stabilizing agent; the seminal plasma stock solution is seminal plasma obtained by directly centrifuging human fresh seminal fluid which is completely liquefied; a mixing volume ratio of the quality control preserving fluid to the seminal plasma stock solution is (1:100)-(100:1). According to the seminal plasma compound quality control product, a special quality control preserving fluid is adopted and freeze-dried after being mixed with the seminal plasma in a proper volume ratio, and then various biochemical criterions in the seminal plasma are more stable, thus the defects of instability and difficulty in preservation of materials in the seminal fluid in vitro are effectively prevented; the prepared seminal plasma compound quality control product is stable in performance, is simple and convenient to use, and can be used in quality control of seminal plasma biochemical detection items.