Automatic portable paper-based micro-fluidic photoelectric detection device and method of pesticide residue

Abstract

The invention belongs to the field of crop pesticide residue detection and biochemical detection, and discloses an automatic portable paper-based micro-fluidic photoelectric detection device and method of pesticide residue. A paper-based chip carrying an indophenol acetate reagent in a first test paper box is conveyed by a chip replacing and conveying belt to a chip installing strip, a paper-based chip carrying an acetylcholin esterase reagent in a second test paper box is conveyed by a chip outgoing and conveying belt to a lower installing strip of an acetylcholin esterase paper-based micro-fluidic chip; a reagent enters a sampling tank through a sampling port, enters an acetylcholin esterase fixed groove under the effect of a paper-based chip sampling channel and is mixed with the acetylcholin esterase; the chip installing strip is driven to move downwards by pressing a component, so that the indophenol acetate reagent and the acetylcholin esterase reagent are in full contact with each other and generate color reaction, and a color region is detected by a photoelectric detection device. The automatic portable paper-based micro-fluidic photoelectric detection device and method are based on an enzyme inhibition reaction principle, obviously improve the detection resolution and sensitivity, and realize an automatic process from inputting a sample to be detected to displaying the amount of pesticide residue.

Description

technical field [0001] The invention belongs to the fields of crop pesticide residue detection and biochemical detection, and applies microfluidic technology to a detection device and method for pesticide residue detection. Background technique [0002] Crop pesticide residues have become a hidden killer that endangers life and health. At present, the pesticide residues in fruits, vegetables, aquatic products, soil and other agricultural products are most prominent in the excess of organophosphorus pesticides and carbamate pesticides. For the detection and management of organophosphorus and carbamate pesticides, avoiding the entry of products containing excessive pesticides into the market has become the most important issue at present. [0003] At present, the rapid detection methods of pesticide residues can be roughly divided into enzyme inhibition method, spectral detection method and chromatographic detection method according to the principle. Spectrometry and chromat...

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Problems solved by technology

[0005] The purpose of the present invention is to design an automatic and portable paper-based microfluidic pesticide residue photoelectric detection device and method for the shortcomings of the current pesticide residue detection method that cannot perform automatic processing of the detection object and the equipment is not easy to carry, with automatic sample pretreatment With detection function, simple structure, easy operation and high degree of automation

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