111 results about "Immunosorbents" patented technology

Immunosorbents, kits and compositions for diagnosing a central nervous system disorder, particularly paroxysmal cerebral discharges and epilepsy, comprising measuring the concentration of GluR1 or fragment thereof and/or GluR1 antibodies in a biological sample from a human subject. The method is particularly useful for identifying individuals that are at risk for brain related seizures and epilepsy, for distinguishing epilepsy from pseudo-epilepsy and epilepsy-like disorders, for following up after anticonvulsive treatment, and for the adjustment of adequate therapy and doses.

A liquid micro drop control chip used in virus detection consists of top and bottom substrates as well as support structure. It is featured as preparing said substrates and said structure by micromechanical means; forming bottom substrate by bottom base plate, electrode array and insulated hydrophobic layer; forming top substrate by top base plate and hydrophobic layer; setting liquid channel, liquid storage region and detection region between top and bottom substrates.

The invention discloses a preparation of polyclonal antibody of heavy metal cadmium, and a measurement method for enzyme linked immunosorbent. The preparation of polyclonal antibody of heavy metal cadmium includes steps of : (A) synthesis of complete antigen, which is critical for preparation of the polyclonal antibody; (B) preparation of the antibody. The measurement method for enzyme linked immunosorbent includes: (1) determining optimal reaction concentration of coating source Cd-ITCBE-OVA and antibody by matrix titration, and coating 96-well enzyme label plate by coating source Cd-ITCBE-OVA with optimal concentration; (2) direct competitive enzyme-linked immunosorbent assay; (3) examining impact of various reaction condition on CI-ELISA, and optimizing reaction condition; (4) establishing CI-ELISA, drawing standard inhibition curve under optimized reaction condition. Detection limit of the invention is 0.04 mu g/L, linear range is 10-1-103mu g/L. The invention is specific to cadmium, suitable for measurement of trace cadmium in ambient water. The invention also provides technical support in fast in-situ detection for sudden accident of heavy metal pollution, in order to carry out remedy and recovery for pollution accident in time.

The invention discloses an immunosorbent comprising a solid-phase carrier and an antibody coupled with the solid-phase carrier. The antibody comprises a monoclonal antibody secreted by a hybridoma cell line CGMCC NO.5504 and a monoclonal antibody secreted by a hybridoma cell line CGMCC NO.5505. The invention also provides a kit comprising the immunosorbent or an immunoaffinity column. Also, the invention provides applications of the immunosorbent, immunoaffinity column, and kit in detecting aflatoxins, sterigmatocystin, zearalenone congeners and ochratoxin A. The invention specifically provides separation and detection methods. According to the invention, the specific monoclonal antibodies with stable performances are developed, such that simultaneous purification and detection of 6 aflatoxins, sterigmatocystin, 6 zearalenone congeners and ochratoxin A are realized.

The invention discloses a composite affinity column for purifying 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone, a preparation method and an application thereof. According to the invention, by employing 4% of cylindrical agarose gel as a solid phase carrier, agarose gel and an antibody are coupled to form an immunoadsorbent, and filling in a column to prepare the immunization affinity column. When a sample containing 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone passes through the immunization affinity column, 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone can be specifically adsorbed by the immunoadsorbent, other impurities flows out of the immunization affinity column, then methanol is used for eluting 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone from the column, so that the sample can be better purified.

The present invention relates to an immunoadsorbent and a composite affinity column for purification of fumonisin B1, aflatoxin, ochratoxin A and zearalenone. The immunoadsorbent comprises a solid carrier and fumonisin B1, aflatoxin, ochratoxin A and zearalenone monoclonal antibodies coupled with the solid carrier, the fumonisin B1 monoclonal antibody is secreted from hybridoma cell line Fm7A11 with the preservation number of CCTCCNO.C201636, the hybridoma cell line Fm7A11 is preserved in CCTCC in March 29, 2016 in Wuhan University Wuhan China. The composite affinity column is loaded with the immunoadsorbent with the fumonisin B1, aflatoxin, ochratoxin A and zearalenone monoclonal antibodies, and is used for purification of the fumonisin B1, aflatoxin, ochratoxin A and zearalenone. The composite affinity column can be used for purification and detection of fumonisin B1, aflatoxin, ochratoxin A and zearalenone toxin samples.

The invention discloses an affinity absorption column for specially removing virions from blood and preparing method thereof, in particular relates to an affinity absorption column for blood purification. The affinity absorption column comprises an absorption carrier, an activator and antivirus surface protein antibody; the activator is added into the absorption carrier to obtain an activated carrier; the antivirus surface protein antibody is added into the activated carrier to perform antibody coupling to obtain an immune absorber, and the immune absorber is loaded into the absorption column to obtain the affinity absorption column. The affinity absorption column has the advantages of strong specificity, little toxic side effect, good therapeutic effect, good repeatability and low cost, and is suitable for blood purification; besides, being a separation technology, the affinity absorption column can be widely applied to other fields for the purification and separation of virus antibodies.

The invention belongs to the field of biological engineering, and provides a kit for in vitro detection of an anti-cyclic citrullinated peptide (CCP) antibody, which comprises a detection plate, a CCP antigen, a colloidal gold-labeled recombinant gold staphylococcus aureus A protein conjugate, a confining liquid, a washing liquid, a positive reference product and a negative reference product. The invention also provides a preparation method of the kit for the in vitro detection of the anti-CCP antibody. The kit adopts an indirect method immunosorbent assay principle to detect the anti-CCP antibody in a human serum, the CCP antigen is coated on a nitrocellulose membrane to be made into a solid-phase antigen for capturing the anti-CCP antibody in the human serum, and then colloidal gold is labeled on staphylococcus aureus A protein (SPA) to form the colloidal gold conjugate as a tracer; and if the detected human serum contains the anti-CCP antibody, then a solid phase antigen-anti-CCP antibody-colloid gold conjugate is formed and has red spots. The invention can be applied to the auxiliary diagnosis of rheumatoid arthritis.

The invention relates to an immunoadsorbent for purifying five kinds of mycotoxins including fumonisin B₁ and aflatoxin B₁, and a complex affinity column. The immunoadsorbent comprises a solid-phase support, and an anti-fumonisin B₁ monoclonal antibody, an anti-aflatoxin B₁ monoclonal antibody, an anti-ochratoxin A monoclonal antibody, an anti-zearalenone monoclonal antibody and an anti-sterigmatocystin monoclonal antibody which are coupled to the solid-phase support, wherein the anti-fumonisin B₁ monoclonal antibody is secreted by a hybridoma cell strain Fm7A11, and the hybridoma cell strain Fm7A11 has been preserved at China Center for Type Culture Collection, Wuhan University, Wuhan, China on Mar. 29, 2016 with the preservation number of CCTCC No. C201636. The complex affinity column can be used for purification and detection of a fumonisin B₁ sample, an aflatoxin B₁ sample, an ochratoxin A sample, a zearalenone sample and a sterigmatocystin sample at the same time.

A method for preparing a carbonized resin DNA immunoadsorbent, which uses styrene, acrylonitrile, divinylbenzene, toluene, liquid paraffin, benzoyl peroxide and water solution of polyvinyl alcohol as raw materials, produces a DNA immunoadsorbent with 0.3-0.5 mg DNA per milliliter of resin using two-staged procedures for preparation of first carbonized resin, and secondly carbonized resin DNA immunoadsorbent. The resultant immunoadsorbent is high in adsorbent capacity, low in production cost, and can be synthesized without pyrogens. It therefore satisfies the demands of medical application for the treatment of systemic lupus erythematosus by immunoadsorption.

The present invention is directed to a method and apparatus for an immunoassay technique that uses amperometric measurements to rapidly analyze different pathogenic microorganisms, including bacteria, viruses, toxins, and parasites and chemical compounds using a disposable element. In accordance with one aspect of the present invention, at least one conductive immunosorbent is used to provide support for antibody immobilization and placed on the top of the working electrode; it could also be used by itself as a working electrode. This immunosorbent or powder can be fabricated of conductive material or nonconductive material over which a conductive material is coated. A membrane cover of the working electrode forms a fluidic chamber having a pore size that is suited to the particular application to insure no contact between the working electrode and counter or silver electrode. The immunoassay can be automated using microprocessor control to reduce the amount of human intervention.

The invention belongs to the field of biological engineering, and provides a kit for in vitro detection of a GPI (Glucose-6-Phosphate Isomerase) antigen, which comprises a detection plate, a goat anti-GPI antibody, a colloidal gold conjugate, a confining liquid, a washing liquid, a positive reference product and a negative reference product. The invention also provides a preparation method of the kit for the in vitro detection of the GPI antigen. The kit adopts a double antibody sandwich colloidal gold immunosorbent assay method principle to detect the GPI antigen in a human serum, the goat anti-GPI antibody is coated on a nitrocellulose membrane to be made into a solid phase antibody for capturing the GPI antigen which possibly exists in a human peripheral serum, and then colloidal gold is labeled on the goat anti-GPI antibody to form the colloidal gold conjugate as a tracer; and if the detected human serum contains the GPI antigen, then a solid phase goat anti-GPI antibody-GPI antigen-colloid gold conjugate is formed. The invention can be applied to the auxiliary diagnosis of rheumatoid arthritis.

The invention discloses an immune affinity precipitation method for purification of antibodies. The immune affinity precipitation method comprises that copolymer solid-phase microballoons containing acrylamide and acrylic acid as monomers are used as solid-phase carriers; antigens are connected to the solid-phase carriers by a coupling agent of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) so that an immunosorbent is formed; an antibody stock solution needing to be purified is mixed with the immunosorbent and the mixture undergoes a full reaction to produce an antibody-immunosorbent conjugate; the antibody-immunosorbent conjugate is subjected to centrifugal washing so that other unbound mixtures are removed and a pure antibody-immunosorbent conjugate is obtained; a system pH value is reduced so that antibodies are dissociated in the solution; the solution with the dissociated antibodies is subjected to centrifugal separation; and a supernatant is collected and then is dialyzed so that the purified antibodies are obtained. The immune affinity precipitation method has simple processes, is suitable for volume production and realizes preparation of antibodies of which purity reaches to an immune affinity chromatography level.

The invention discloses a novel coronavirus SARS-CoV-2 detection proteome and an application thereof. A detection method for detecting novel coronavirus SARS-CoV-2 is an enzyme-linked immunosorbent assay method. The N protein is optimized, and the receptor binding region (RBD 331aa-532aa) of the S protein is selected for expression through structural analysis aiming at the S gene of the novel coronavirus, so that the detection specificity can be further improved; an enzyme-linked immunosorbent assay method is used, and whether serum to be detected is positive or not can be efficiently and accurately determined by optimizing conditions such as coating concentration and time, primary antibody and secondary antibody incubation time and developing time.

An aflatoxin M1 nanobody, an immunosorbent and an immunoaffinity column. The aflatoxin M1 nanobody 2014AFM-G2 has the amino acid sequence of SEQ ID NO:7, is encoded by the nucleic acid sequence of SEQ IDNO:8, has a 50% inhibiting concentration IC₅₀ to aflatoxin M1 of 0.208 ng/mL, and has cross reaction rates with aflatoxins B1, B2, G1, and G2 of 9.43%, 5.93%, 4.87% and 6.17%, respectively. The immunosorbent includes a solid phase carrier and aflatoxin M1 nanobody 2014AFM-G2 coupled with the solid phase carrier. The immunoaffinity column is loaded with the aflatoxin M1 nanobody immunosorbent. It can be used for purifying and concentrating an extracting solution of a sample before loading to a machine for detection and the immunoaffinity column can be used repeatedly for many times.

The invention discloses a hybridoma cell strain CGMCC NO. 5506 and a monoclonal antibody obtained by secretion of the cell strain. The invention also provides an immunoadsorbent comprising a solid phase vector and an antibody coupled with the solid phase vector, wherein the antibody is the above monoclonal antibody; and an immunoaffinity column loaded with the immunoadsorbent. The invention also provides a kit containing the above immunoadsorbent or the above immunoaffinity column, and applications of the above immunoadsorbent, the above immunoaffinity column and the kit in detecting aflatoxins and sterigmatocystins. Besides, the invention provides a separation method and a detection method specifically. The monoclonal antibody with stable and specific performance is developed by the invention, so that purification and detection for six aflatoxins and sterigmatocystins at the same time are realized.

The invention discloses an immunosorbent for removing inflammatory factors in blood and a preparation method thereof. Based on a variety of intermolecular interactions between the antigenic epitope ofinflammatory factors such as IL-6, TNF-alpha, IL-1 beta and IL-8 and corresponding receptors thereof, affinity ligand of small molecule polypeptide with 5-18 selective amino acids is designed througha computer-assisted drug design method. Specifically, with vinyl acetate-triallyl isocyanurate as a basic skeleton, a mixture of ethyl acetate and alkane as a pore-forming agent, and a synthetic resin with benzoyl peroxide as an initiator as a carrier, alcoholysis activation is carried out, and then the small molecule polypeptide is grafted to obtain the small-molecule ligand immunosorbent whichdoes not adsorb macromolecular proteins and the like. The immunosorbent is simple to prepare, has high adsorbing capacity, high selectivity, good biological and blood compatibility, and relatively lowcost, and provides a new therapeutic method for removing excessive pathogenic inflammatory factors in patients.