391results about How to "Low cross-reactivity" patented technology

The invention relates to a chemiluminescence quantitative detection kit for procalcitonin, and a preparation method and detection method thereof. The kit comprises a procalcitonin series standard substance, a magnetic separation reagent (magnetic particle suspension coupled with streptavidin), a first reagent (anti-procalcitonin monoclonal antibody solution containing biotin N-hydroxysuccinimide ester label) and a second reagent (anti-procalcitonin monoclonal antibody solution containing alkaline phosphatase label). The sensitivity of the kit prepared from the magnetic particle suspension coupled with streptavidin, anti-procalcitonin monoclonal antibody solution containing biotin N-hydroxysuccinimide ester label and anti-procalcitonin monoclonal antibody solution containing alkaline phosphatase label is up to 0.008ng / ml; and the kit has the advantages of high accuracy, high precision, no need of prediluting the sample, and wide detection range, and is simple and time-saving to operate.

The present invention relates to novel adenovirus strains with an improved sero-prevalence. In one aspect, the present invention relates to isolated polypeptides of adenoviral capsid proteins such as hexon, penton and fiber protein and fragments thereof and polynucleotides encoding the same. Also provided is a vector comprising the isolated polynucleotide according to the invention and adenoviruses comprising the isolated polynucleotides or polypeptides according to the invention and a pharmaceutical composition comprising said vector, adenovirus, polypeptide and / or polynucleotide. The invention also relates to the use of the isolated polynucleotides, the isolated polypeptides, the vector, the adenoviruses and / or the pharmaceutical composition for the therapy or prophylaxis of a disease.

Peptide antigens corresponding to amino acid residues 2-12, 1-12, 2-15 and 1-15 of parathyroid hormone (PTH), antibodies having an affinity to such peptide antigens and methods of producing the same. Such antigens, antibodies and methods producing the same according to the present invention are useful in determining bioactive intact PTH levels in serum, plasma, and / or cell culture media. Such antibodies further possess a high degree of species cross-reactivity, but substantially mitigated cross-reactivity to non-whole PTH peptide fragments and little to no recognition of the first amino acid residue of PTH.

The present invention relates generally to isolated to arginine deiminase (ADI) proteins that have reduced cross-reactivity with anti-ADI-PEG 20 antibodies as compared to ADI-PEG 20, but which can have functional characteristics comparable to or better than ADI-PEG 20, compositions comprising the ADI proteins, and related methods of treating arginine-dependent diseases or related diseases such as cancer.

Production of triazophosphous artificial semi-antigen, antigen and antibody is carried out by taking O-ethyl thiophsphoryl dichloride as material, reacting O-ethyl thiophsphoryl dichloride with synthetic intermediate 1-phenyl-1,2,4-triazoalcohol of triazophosphous under catalyst action, generating monochloride, reacting monochloride with propalanine under acid-binding agent action, generating triazophosphous semi-antigen TZLBu, reacting monochloride with amino acetic acid under acid-binding agent action, generating TZLHe, coupling with protein by carbodi-imide method and mixed acid anhydride method, and obtaining artificial antigen. Its advantages include rapid, convenient, low cost, and specific antibody with high affinity.

The present invention provides a sensor cartridge (10) for distinctively determining at least two different target moieties in a fluid sample. The sensor cartridge (10) comprises a reaction chamber (1) and at least a first and second region (2, 3) distinct from each other. The first region (2) comprises magnetic or magnetizable objects (4a) labelled with a first type of probes for specifically binding a first type of target moieties and the second region (3) comprises magnetic or magnetizable objects (4b) labelled with a second type of probes for specifically binding a second type of target moieties, the magnetic or magnetizable objects (4a, 4b) in the first and second region (2, 3) being directly contactable by the sample fluid. The present invention also provides a method for the manufacturing of such sensor devices (10) and a method for determining the presence and / or amount of at least two different target moieties in a sample fluid using such sensor cartridge (10).

The invention relates to a single-chain antibody against fenitrothion and a preparation method thereof. A ribosome display library of a whole set of single-chain antibody genes against the fenitrothion is constructed by using a ribosome display technology and rounding a hybrid tumor technology, and three single-chain antibody genes with high affinity and high specificity for the fenitrothion are screened from the library. After the single-chain antibody genes are connected with vectors PPOW3.0 and then subjected to soluble expression in escherichia coli, three soluble proteins with molecular weights of about 30KDa are obtained; and by enzyme-linked immuno sorbent assay (ELISA) and Biacore analysis, the three antibody proteins have high affinity and high specificity for the fenitrothion, so excellent single-chain antibody genes and antibody proteins are provided for establishing ELISA and immune sensors. The invention has significance for realizing quick, simple, convenient and accurate detection of organic phosphorus pesticide residue, and provides a new direction.

The invention provides a hybridoma cell strain 2C9, an anti-aflatoxin M1 monoclonal antibody produced by the secretion of the hybridoma cell strain 2C9 and application thereof. The hybridoma cell strain 2C9 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO.C201018. The hybridoma cell strain 2C9 can be used for preparing high-titer anti-aflatoxin M1 monoclonal antibody, and a method of rat ascites antibody ELISA (Enzyma-linked Immunosorbent Assay) is adopted to detect the titer which can reach 4.26*106. The ant-aflatoxin M1 monoclonal antibody has high sensitivity, the 50% of inhibition concentration IC50 aflatoxin M1 caused by the anti-aflatoxin M1 monoclonal antibody is 67pg / ml, and the cross reaction rates between the anti-aflatoxin M1 monoclonal antibody and aflatoxin B1, between the anti-aflatoxin M1 monoclonal antibody and aflatoxin B2, between the anti-aflatoxin M1 monoclonal antibody and aflatoxin G1 and between the anti-aflatoxin M1 monoclonal antibody and aflatoxin G2 are respectively less than 0.1%. The anti-aflatoxin M1 monoclonal antibody can be used for quick detection of aflatoxin M1.

This invention uses immunity compatible chromatography post and enzyme linked immunosorbent assay to detect the remain RactopamineHy-drochloridie in food. It belongs to immunology and food safety detect technology. The method is: prepare muti-clone antibody by immunity antigen synthesis, antigen peridium, animal immunity and antigen depuration. Then put the antigen on to agarose gel to prepare immunity compatible chromatography post. You can enrich the RactopamineHy-drochloridie in the sample by compatible post and gather the eluent of the compatible post, then use linked immunosorbent assay to determine the consistency of the RactopamineHy-drochloridie.

The invention discloses a prolactin (PRL) time-resolved fluoroimmunoassay method and a kit. The method comprises the following steps: selecting and using a monoclonal antibody against PRL as a coated antibody, and diluting sodium carbonate buffer solution to 1 to 10mu g / ml as coating liquid; preparing a lanthanide ion label against the PRL monoclonal antibody as a labeled antibody; diluting and using the reaction buffer solution according to a ratio of 1 to 20 during experiment; and on a reaction plate of the coated antibody, adding 25mu l of standard substance of PRL or sample to be detected and the diluted labeled antibody into each hole in turn, and carrying out fluorescence detection after hatching. The invention also provides the corresponding kit. The method and the kit have the advantages of high sensitivity, specificity and stability; and the high automated assay system can improve the speed of clinical examination result, greatly reduce human error and increase the reliability of detected result.

The invention relates to a kit for rapidly diagnosing lipoprotein-associated phospholipase A2 and a use method of the kit. The kit comprises a standard substance, a quality control substance, a liposome complex, a magnetic separation reagent, an assay buffer solution and a cleaning solution, wherein the inner part of the liposome complex is coated with N-hydroxysuccinimide ester of tris (bipyridine) ruthenium; an antibody for resisting the lipoprotein-associated phospholipase A2 is connected to the surface of the liposome complex by interaction of streptavidin and biotin; the magnetic separation reagent takes magnetic particulates containing amino terminals as a carrier and is connected with the antibody for resisting the lipoprotein-associated phospholipase A2 through the interaction of the streptavidin and the biotin. Compared with an enzyme linked immunosorbent assay kit produced in a foreign Diadexus company, the kit prepared by the invention has the advantages that sensitivity and precision for analyzing the lipoprotein-associated phospholipase A2 are greatly improved; in addition, the kit disclosed by the invention is simple and convenient to operate, quick and easier to popularize.

The invention relates to an enrofloxacin monoclonal antibody and application, relates to hybridoma strains thereof, and belongs to the technical field of immunochemistry. The enrofloxacin monoclonal antibody is generated by mouse hybridoma strains 6A4 and 8E6. The preparation method comprises the following steps that: enrofloxacin and carrier proteins BSA, HAS and OVA are coupled by a carbodiimide method to synthesize artificial immunogens EnR-BSA, EnR-HSA and coatingen EnR-OVA; a Balb / c mouse is immunized by the synthesized artificial immunogens EnR-BSA and EnR-HSA; a spleen cell of the immunized mouse is extracted to be fused with a SP2 / O myeloma cell and coated by the coatingen EnR-OVA; indirect ELISA method and indirect competition ELISA method are established to screen the hybridoma strains which can stably secrete specific antibody; the obtained cell strain immunized Balb / c mouse is used to prepare ascites; the ascites is purified by a caprylic acid-ammonium method and an ion exchange method; and valences of antibodies of two purified cell strains reach over 1.024*10 and 1.28*10. The monoclonal antibody has strong specificity, can be applied to preparation of enrofloxacin residue inspection kit and aerosol test strip, and can sensibly and quickly inspect the enrofloxacin residue.

The invention discloses an enzyme linked immunosorbent assay kit used for detecting the content of heavy metal copper ions in a sample. The kit includes a coat antigen coated enzyme label plate, an enzyme marker, a copper ion chelate specific monoclonal antibody or polyclonal antibody, a copper ion chelate standard solution, a substrate color development solution, a terminating solution, a lotion, and a diluted chelating agent EDTA. The enzyme linked immunosorbent assay kit used for copper ion detection has the advantages of: (1) strong specificity, high sensitivity, a lowest detection limit of 0.42 microgram / L, and a detection range of 2.83-456.04 microgram / L; (2) simplicity, rapidity, and strong timeliness; (3) vivid, visual and accurate result display; and (4) low cost, wide application range, convenient popularization, on-site supervision and suitability for screening a large number of samples.

The invention relates to an indirect competition enzyme linked immunoreagent kit for detecting lead ions and a manufacturing method of the kit. An elisa plate enveloped by lead ion envelope antigens, lead-ion monoclonal antibodies, elisa second antibodies, a substrate color developing solution, a stop solution, a lead ion standard solution, a washing liquor concentrated solution and a sample treating liquid are arranged in the kit. The indirect competition enzyme linked immunoreagent kit can detect the lead irons in a trace mode, is used for detecting the lead iron contamination residual in the environment, the soil, water, foods and containers, and has the advantages of being rapid, easy and convenient to use, sensitive, peculiar, economical and the like. The kit is few in detection step and high in timeliness, saves detection time, reduces operation errors and can perform field detection. The kit not only can be used for screening samples in large batch, but also can perform rapid detection on samples in small batch, and provides effective means for food import and export inspection, food inspection, monitoring and evaluation of environmental pollution and the like.

The invention provides an enzyme linked immunosorbent assay kit for detecting content of heavy metal zinc ions in a sample, and the enzyme linked immunosorbent assay kit comprises an elisa plate with a coating antigen, an enzyme marker, a zinc ion chelate specific antibody, a zinc ion chelate standard substance solution, a substrate coloured solution, a stop solution, a washing concentrate and a concentrated complex solution. The invention also provides a method for detecting a zinc ion chelate by the enzyme linked immunosorbent assay kit, and the method comprises the following steps: firstly, carrying out sample pretreatment; then carrying out detection by the kit; and finally, analyzing a detection result. The invention provides the enzyme linked immunosorbent assay kit and the detection method for detecting the residual quantity of the zinc ions in the samples such as foods, feeds, beverages, soil, animal tissues and the like; the operation is simple and convenient; the cost is low; the flexibility is high; and the enzyme linked immunosorbent assay kit can be used for field monitoring and is suitable for screening a great amount of samples.

The invention belongs to the field of in-vitro diagnostic reagent kits, and particularly relates to a myoglobin detection reagent kit and a method for applying the same. The myoglobin detection reagent kit comprises calibration products, reagents R1, enzyme conjugate working solution R2, magnetic bead conjugate working solution M, cleaning solution and chemiluminescent substrates. The reagents R1contain imidazole components, blood cells in whole blood can be quickly removed by the imidazole components, and accordingly the possibility that magnetic beads are swallowed by the blood cells can beeffectively prevented; the cleaning solution contains sodium lauryl sulfate components, accordingly, cleaning effects can be enhanced, and non-specific binding of the chemiluminescent substrates canbe prevented. The myoglobin detection reagent kit and the method have the advantages that finger peripheral whole blood or anticoagulant venous whole blood can be directly used as a to-be-detected sample for the myoglobin detection reagent kit, the whole blood sample can be directly detected without being pretreated, accordingly, the detection speed can be greatly increased, operation steps can besimplified, the application range of the myoglobin detection reagent kit can be expanded, and large-scale popularization and application can be facilitated.

The invention discloses an NRAS gene mutation detection specificity primer and a liquid chip thereof. The liquid chip mainly comprises: an ASPE primer composed of a 5'-terminal tag sequence and 3'-terminal specificity primer sequences focused on target gene mutation sites, wherein the specificity primer sequences comprise SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21 and / or SEQ ID NO.22 focused on a Codon12 site, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26 and / or SEQ ID NO.27 focused on a Codon13 site, SEQ ID NO.28 and SEQ ID NO.29 focused on a Codon18 site, and / or SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33 and / or SEQ ID NO.34 focused on a Codon61 site; a microsphere coated by an anti-tag sequence; and an amplimer. The consistency between the detection result of the detection liquid chip provided by the invention and the detection result of a sequencing method is high to 100%, and the wild-type and mutant parallel detection of a plurality of mutation sites is realized.

Triazophosphous artificial semi-antigen, antigen, specific antibody and its use are disclosed. N=1-10 in molecular structural formula. It achieves high titer, specificity and affinity.

The invention discloses an immunochromatographic test strip for detecting cardiac infarction and heart failure and a preparation method thereof, and is intended to provide an immunochromatographic test strip that provides detection results accurately determinable and causes few cross reactions. The immunochromatographic test strip comprises a bottom board; a sample pad, a binding pad, a reaction film and an absorbing pad are linked to the bottom board; CRP (C-reactive protein) antibody and chicken IgY labeled by 300 nm immunofluorescence spheres are sprayed to the binding pad, and NT-proBNP antibody labeled by 200 nm immunofluorescence spheres is also sprayed thereto; the reaction film is parallelly provided with a C quality control line, a T1 detection line and a T2 detection line; the C quality control line is coated with goat anti-chicken IgY; the T1 detection line is coated with antigen CRP protein; the T2 detection line is coated with anti-NT-proBNP antibody.

The present invention relates to a method for in vitro evolution of protein function. In particular, the method relates to the shuffling of nucleotide segments obtained from exonuclease digestion. The present inventors have shown that polynucleotide fragments derived from a parent polynucleotide sequence digested with an exonuclease can be combined to generate a polynucleotide sequence which encodes for a polypeptide having desired characteristics. This method may be usefully applied to the generation of new proteins (e.g., antibodies and enzymes) or parts thereof having modified characteristics as compared to the parent protein.

The invention discloses a O, O-dimethyl-O-[-3-methyl-4-amino phenyl] thionic phosphate (FNH2) as fenitrothion hapten, artificial antigen and specific antibody, which is characterized by the following: reducing nitro in the fenitrothion molecular structure into amino under acid condition with zinc powder; reacting to obtain the hapten molecule in connection with carrier protein; diazotizing to couple hapten and protein to obtain immunogen and peridium; using immune animal with immunogen to make the specific antibody.

The invention discloses luteinizing hormone (LH) time-resolved fluoroimmunoassay and a kit. In the luteinizing hormone (LH) time-resolved fluoroimmunoassay, an anti-LH monoclonal antibody is used as a coating antibody and diluted by a sodium carbonate buffer solution to 1-10 ug / ml to serve as a coating solution, a lanthanide ion label paired with the anti-LH monoclonal antibody serves as a label antibody and is diluted by reaction buffer solution in a ratio of 1:20 for use during experiment, and 25mu liters of an LH standard product or a sample to be detected as well as the diluted label antibody is filled in holes on a reaction plate coated by the antibody sequentially for fluorescence detection after incubation. The invention also provides the corresponding kit. The kit has high sensitivity, specificity and stability; and the luteinizing hormone (LH) time-resolved fluoroimmunoassay adopts a high automatic analysis system and can improve clinic detection result generation speed and greatly reduce human errors and improve the reliability of the detection result.

The invention relates to an indirect competitive enzyme linked immunosorbent assay (elisa) kit for detecting chromium ions as well as preparation and detection methods thereof. A trivalent chromic ion envelope antigen-enveloped elisa plate, a trivalent chromic ion-resistant monoclonal antibody, a second enzyme-labeled antibody, a substrate color-developing solution, a stop solution, a trivalent chromic ion standard solution, a lotion concentrated solution and a sample processing solution are arranged in the kit. Samples are processed before detection so that Cr3<+> in the samples forms Cr<3+>-EDTA (ethylene diamine tetraacetic acid), and then the chromium ions are detected by the kit. The chromium ion enzyme linked immunosorbent assay kit can be used for detecting the trace amount of chromium ions, has the Cr<3+> detection sensitivity of 2ng / ml, has the characteristics of quickness, simplicity and convenience, sensitivity, specificity, economy and the like, has few detection steps, saves the detection time, and reduces the operation error; the detection cost is less than 1 / 20 of that of a physical and chemical analysis method, the timeliness is high, the field detection can be performed, and the kit is mainly used for screening large-batch samples of chromium ion pollution residues in environments, soil, water and foods.

The invention discloses a method for preparing a complete antigen from a trimethoprim semiantigen compound T1 and a use of the complete antigen, and belongs to the technical field of biochemical engineering. An amino group at one end of a pyrimidine nucleus is derived to form active carboxyl, a trimethoprim carboxyl derivative (TMP-HS) with a single specific structure is obtained by purification, the derivative is the trimethoprim semiantigen compound T1, and the trimethoprim semiantigen compound T1 is coupled to a carrier protein to form the trimethoprim complete antigen T1-MA-BSA. The antigen or antibody can be used for building an enzyme-linked immunosorbent assay adsorption analysis method and a colloidal gold test paper rapid-detection method so that trimethoprim residue in foods can be fast detected.

The invention provides an aflatoxin B2 aptamer affinity column and a preparation method thereof, wherein the affinity column uses cyanogens-bromide-modified agarose as a vector, a nucleic acid aptamercapable of recognizing aflatoxin B2 with high affinity and high specificity is covalently coupled to the vector, and the coupled aflatoxin B2 aptamer complex vector is loaded into the affinity column. The affinity column is mainly used for purification and cleaning of the aflatoxin B2 in food, feeds, milk, blood samples, traditional Chinese medicines and other various samples so as to facilitatehigh performance liquid chromatography and fluorescence detection of the aflatoxin B2 in the samples.

The invention discloses a human immunodeficiency virus (HIV) (type 1+2) antibody detection double-antigen sandwich time resolved fluoroimmunoassay method and a kit. Recombinant niobium is used as a coating antigen, and sodium carbonate buffer solution is diluted to 1 to 10mu g / mL to form coating solution; paired HIV recombinant antigens are labeled as labeled antigens by using lanthanide elements; in an experiment, the reaction buffer solution is diluted for use in a ratio of 1:21; and 100mu L of HIV negative or positive control or sample to be detected and the diluted labeled antigens are sequentially added into each pore of a reaction plate of coating antibodies, and fluorescence detection is performed after incubation. The invention also provides the corresponding kit. The kit has relatively high sensitivity, specificity and stability; and because the analysis system has high automation, the speed of the clinical inspection result can be improved, human errors can be greatly reduced, and the reliability of the detection result is increased.

The present invention relates to novel adenovirus strains with an improved seroprevalence. In one aspect, the present invention relates to isolated polypeptides of adenoviral capsid proteins such as hexon, penton and fiber protein and fragments thereof and polynucleotides encoding the same. Also provided is a vector comprising the isolated polynucleotide according to the invention and adenoviruses comprising the isolated polynucleotides or polypeptides according to the invention and a pharmaceutical composition comprising said vector, adenovirus, polypeptide and / or polynucleotide. The invention also relates to the use of the isolated polynucleotides, the isolated polypeptides, the vector, the adenoviruses and / or the pharmaceutical composition for the therapy or prophylaxis of a disease.

The present invention relates generally to isolated to arginine deiminase (ADI) proteins that have reduced cross-reactivity with anti-ADI-PEG 20 antibodies as compared to ADI-PEG 20, but which can have functional characteristics comparable to or better than ADI-PEG 20, compositions comprising the ADI proteins, and related methods of treating arginine-dependent diseases or related diseases such as cancer.

The invention, which belongs to the technical fields of immunology and food safety analysis, relates to a colloidal gold chromatography test strip for detecting skeletal muscle troponin I of cattle orsheep, and a preparation method and application thereof. The test strip comprises a bottom plate, a sample pad, a colloidal gold pad, a coating film and an absorbent pad. Protective films are arranged at two ends of the test strip. The sample pad, the colloidal gold pad, the coating film and the absorption pad are pasted on the bottom plate successively. The colloidal gold pad is coated with a colloidal-gold-labeled capture antibody. The coating film is provided with an invisible detection line printed by a detecton antibody solution and an invisible quality control line printed by a goat anti-mouse IgG solution. The capture antibody is obtained by secretion of a hybridoma cell strain 3A8 having the preservation number of CCTCC NO:C2018217; and the detection antibody is obtained by secretion of a hybridoma cell strain with the preservation number of CCTCC NO:C2018218. The colloidal gold chromatography test strip having characteristics of great convenience, rapidity, visibility, strongtimeliness, wide application range, and low cost is convenient to promote and apply.

The invention discloses a preparation method and an application of a magnetic graphene oxide-nanogold label-free complex. On the basis of different adsorption effects of magnetic graphene oxide on single-stranded DNA and double-stranded DNA, after small target RNA is added, an identification section of DNA1 is specifically bound to the small target RNA, so that a DNA / RNA double-stranded structure is formed and a gold nanoprobe, which is adsorbed on the surface of the magnetic graphene oxide, is dissociated in a solution. Through magnetic separation, on the basis of a developing effect of nanogold in a visible light region, the color change of the nanogold solution in a supernatant, along with different small RNA concentrations, is observed; and through quantitative analysis by virtue of an ultraviolet-visible spectrophotometer, rapid colorimetric detection, with high sensitivity and high specificity, on the small RNA is achieved.