47results about How to "Retain chemical structure" patented technology

The invention provides a preparation method of a sildenafil artificial antigen and antibody, and relates to a preparation method of an artificial hapten, antigen and antibody maximizing the retention of a sildenafil structure. A sildenafil hapten prepared by a chemical synthesis method and a carrier KLH protein are connected and synthesized into an artificial antigen; and an antibody is prepared by the following steps: immunizing animals with the artificial antigen, taking blood, separating out antiserum and purifying. By designing and synthesizing the hapten and artificial antigen having a sildenafil structure, the invention highlights the molecule-specific antigenic determinant, overcomes difficulties in chemical synthesis, and produces the high-affinity antibody through animal immunization inducement. Compared with other similar methods, the invention has the characteristics of specificity, sensitivity, accuracy, high speed, convenience, low cost and the like; and the designed and synthesized hapten lays a foundation for the preparation of the antibody having favorable specificity.

The invention discloses a semi-antigen and a preparation method and application thereof and specifically relates to an amikacin semi-antigen. The invention further discloses a preparation method and application of the amikacin semi-antigen. A rapid detection kit product established on the basis of the amikacin semi-antigen has the advantages of convenient usage, low detection cost, usage of highly efficient, accurate and rapid detection methods and capacity of simultaneous on-site monitoring of amikacin residue and screening of considerable samples.

The invention discloses a hapten, and a preparation method and application thereof, particularly a sulfonamide hapten. The invention also discloses a preparation method and application of the hapten. The kit quick detection product established on the basis of the sulfonamide hapten is convenient to use and low in detection cost; and the detection method is efficient, accurate and quick, can be used for simultaneously detecting mass samples, and is suitable for on-site supervision of sulfonamide residues in animal-derived samples and screening of mass samples.

The invention provides a gemfibrozil hapten and an artificial antigen. Molecular structural formulas of the gemfibrozil hapten and the artificial antigen are correspondingly as shown in the description. The invention further discloses preparation methods for the gemfibrozil hapten, the artificial antigen and an antibody prepared from the gemfibrozil hapten and the artificial antigen and purposes of the gemfibrozil hapten and the antibody. The antibody which is finally prepared by the preparation method provided by the invention has the advantages of high detection sensitivity and high specificity.

The invention discloses a preparation method of a bisphenol A hapten and a bisphenol A complete antigen. The preparation method comprises the steps of enabling bisphenol A, which is taken as a raw material, to react with monochloroacetate in presence of a catalyst-potassium hydroxide / sodium hydroxide, removing the unreacted bisphenol A by using ethyl acetate, then acidizing by using hydrochloric acid to obtain a product, filtering, washing, and drying to obtain the bisphenol A carboxylated hapten; coupling the bisphenol A hapten with a carrier protein OVA by using a carbodiimide method so as to prepare the bisphenol A complete antigen. The preparation method provided by the invention is simple, convenient and rapid, and greatly shortens the reaction time on the basis of guaranteeing the product purity and yield; furthermore, after the preparation method is adopted, heating and pH control are not needed, so that the industrial production cost is lowered.

The invention discloses an estrogen hapten, a complete antigen and a preparation method thereof. The method comprises: reacting estrols and monochloroacetate in an organic solvent at normal temperature and in the presence of a catalyst, terminating the reaction with ice water, taking the unreacted estrols of the organic extractant, and using acid Acidification produces a white precipitate, which is the estrol hapten. By optimizing the catalyst and reaction time in the synthesis, this method provides a simple method without heating and controlling the pH, and the hapten can be obtained in a short time; the unreacted product and the substrate are used to remove the unreacted hapten by using the extraction method. At the same time, the obtained estrols can continue to be used through recovery, saving the reaction substrate; finally, the method of crystallization by hydrochloric acid acidification can obtain high-purity estrols haptens; Haptens are linked to carrier proteins to obtain complete antigens.

The invention discloses a semi-antigen, a preparation method and applications thereof, and specifically relates to a dexamethasone semi-antigen. The invention also discloses a preparation method of the semi-antigen and applications thereof. The rapid detection kit product based on the dexamethasone semi-antigen has the advantages of user-friendliness, low detection cost, high efficiency, accuracy and rapidness, is capable of detecting a large amount of samples at the same time, and is suitable for on-site supervision of dexamethasone residual in the animal source food and screening of a large amount of samples.

The invention discloses a hapten, and a preparation method and application thereof, particularly a methyl parathion hapten. The invention also discloses a preparation method and application of the hapten. The kit quick detection product established on the basis of the methyl parathion hapten is convenient to use and low in detection cost; and the detection method is efficient, accurate and quick, can be used for simultaneously detecting mass samples, and is suitable for on-site supervision of methyl parathion residues in corn and apple samples and screening of mass samples.

The invention discloses an antigen, an antibody and an enzyme-linked immunosorbent assay (ELISA) kit of amantadine. Firstly, an amantadine hapten is prepared, wherein the molecular structural formulaof the amantadine hapten is as shown in formula (I) (The formula is shown in the description.); then, the amantadine hapten is coupled with carrier protein to obtain an artificial antigen; and then, amonoclonal antibody of amantadine is obtained through a hybridoma technique, thereby establishing a competitive ELISA method and kit according to the monoclonal antibody of amantadine. The ELISA kitof amantadine, disclosed by the invention, can be used for detecting the content of amantadine in an animal tissue sample, the detection limit is 0.10mu g / kg, IC50 is 0.79mu g / L, and the linear detection range is 0-16.2mu g / L; and the method is simple, convenient and quick, is low in cost, high in sensitivity and highstrong in specificity, and is suitable for on-site quick detection.

A preparation method and application of a pendimethalin hapten and an antigen, characterized in that: the pendimethalin hapten is composed of N-(1-ethylpropyl)-3,4-dimethylaniline and Ethyl 4‑bromobutyrate is reacted to generate alkylated N‑(1‑ethylpropyl)‑3,4‑dimethylaniline, which is then reacted with concentrated nitric acid to generate nitroalkylated N‑(1‑ethylpropyl) )-3,4-dimethylaniline, and then obtained by reacting with potassium hydroxide; the pendimethalin antigen is obtained by coupling pendimethalin hapten with carrier protein. The antigen prepared by the invention presents specific pendimethalin antigenic determinant, which makes it possible to screen highly specific pendimethalin monoclonal antibodies. The antibody produced has high specificity and high sensitivity, and can be used to establish an enzyme-linked immunosorbent assay method and a rapid determination method of colloidal gold test paper, thereby realizing the rapid detection of pendimethalin in tobacco and food.

The invention discloses a streptomycin hapten, as well as a preparation method and application thereof, and in particular relates to a streptomycin hapten. The invention further discloses a preparation method and an application of the streptomycin hapten. A quick detection enzyme-linked immune kit product built based on the streptomycin hapten is convenient to use, low in detection cost and efficient, accurate and quick in detection, can be used for simultaneously detecting large batches of samples, and is suitable for site monitoring and streptomycin residue in animal derived foods and screening of lots of samples.