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Anti-nandrolone specific antibody

A nandrolone and antibody technology, which is applied in the direction of specific peptides, animal/human proteins, serum albumin, etc., can solve the problems of difficult elimination of matrix influence and complex components, and achieve the effect of low price, simple synthesis method and easy access

Inactive Publication Date: 2012-02-01
TIANJIN UNIV OF SCI & TECH
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AI Technical Summary

Problems solved by technology

Since the addition of 17β-NT in functional foods is very small, and the ingredients of functional foods are generally complex, the influence of the matrix is ​​difficult to eliminate, and it is difficult for conventional detection methods to achieve ideal detection results. Therefore, it is urgent to establish an economical and reliable method. , specific, sensitive, fast and effective method

Method used

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  • Anti-nandrolone specific antibody
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  • Anti-nandrolone specific antibody

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Embodiment 1

[0031] 1. Hapten Synthesis

[0032] The present invention selects the hapten of nandrolone, and its molecular structural formula is:

[0033]

[0034] The specific preparation method is: accurately weigh 0.5488mg nandrolone and dissolve it in 13.5mL anhydrous tetrahydrofuran, add 0.4g succinic anhydride, 0.55mL triethylamine and 0.243mg DMAP, react at 60°C overnight, and evaporate the solvent to dryness.

[0035] 2. Purification

[0036] The resulting residue was dissolved with the organic solvent ethyl acetate / petroleum ether (1 / 1, v / v), and purified by column chromatography. After optimization, ethyl acetate / petroleum ether (1 / 1, v / v) was finally selected as the developer. The obtained filtrate was rotary evaporated to obtain white crystals, namely 17β-NT hapten.

[0037] 3. Synthesis of artificial antigen

[0038] Accurately weigh 0.025 mmol (9.36 mg) of the 17β-NT hapten and 9.3 mg EDC obtained above, respectively, and dissolve them in 1 mL of N-N dimethylformamide ...

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Abstract

The invention provides a preparation method of a nandrolone artificial antigen and antibody, and relates to a preparation method of an artificial hapten, antigen and antibody maximizing the retention of a nandrolone structure. A nandrolone hapten prepared by a chemical synthesis method and a carrier BSA protein are connected and synthesized into an artificial antigen; and an antibody is prepared by the following steps: immunizing animals with the artificial antigen, taking blood, separating out antiserum and purifying. By designing and synthesizing the hapten and artificial antigen having a nandrolone structure, the invention highlights the molecule-specific antigenic determinant, overcomes difficulties in chemical synthesis, and produces the high-affinity antibody through animal immunization inducement. Compared with other similar methods, the invention has the characteristics of specificity, sensitivity, accuracy, high speed, convenience, low cost and the like; and the designed and synthesized hapten lays a foundation for the preparation of the antibody having favorable specificity.

Description

technical field [0001] The present invention relates to selecting a compound that most likely contains the original structure of nandrolone as a nandrolone hapten, and making the hapten into an antigen, and then producing an antibody; and methods for synthesizing the hapten and the antigen and preparing the antibody. The invention belongs to the field of biotechnology. Background technique [0002] With global economic integration and internationalization of food trade, food safety has become a worldwide challenge and an important global public health issue. With the improvement of people's living standards, people pay more and more attention to their health, and functional foods are increasingly favored by people. However, the ensuing adulteration of functional foods is becoming more and more serious, seriously endangering the physical and mental health of consumers. In recent years, the safety problem of functional food has become increasingly prominent, and new requirem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K14/795C07K16/44C07K16/06C07J1/00G01N33/535
Inventor 王硕张燕王俊平王莉
Owner TIANJIN UNIV OF SCI & TECH
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