608results about How to "Simple purification method" patented technology

The invention belongs to the synthesis technical field of organic medicine, and discloses a synthesis method of anti-influenza and avian influenza virus resistant medicine peramivir, which is characterized by adopting (1R, 4S)-2-azabicyclo[2.2.1]hept-5-en-3-one as a raw material and by comprising the following steps: step I: catalyzing, loop-opening and amino protection; step II: 1,3-dipolarcycloaddition; step II: sodium borohydride and nickel chloride hexahydrate reduction and acetylization; and step IV: amino protection removal, and chloroformamidine hydrochloride is adopted as reaction agent to generate guanidyl and hydrolysis methyl ester to prepare peramivir. The sodium borhydride and the nickelous chloride reduction system is used for substituting the expensive platinum dioxide to be used as reducing agent during the hydrogenation reduction process, and the self-produced chloroformamidine hydrochloride is used during the process for feeding the guanidyl reagent, so the cost is reduced, and the operation procedures are simplified. The synthesis method has short reaction routine and moderate reaction condition, is simple and convenient to operate, has low cost, and is suitable for the industrialized production.

Different from the conventional method for purifying human serum albumin from human plasma, the invention provides a simple method for separating and purifying the human serum albumin from fermentation liquor or a mammal cell culture solution. By an immobilized metal affinity chromatography (IMAC) step, the method is suitable for purifying recombinant proteins at various salt concentrations, fermentation supernate at high salt concentration is allowed to be directly loaded, the total volume of loading buffer is effectively reduced and the cost is reduced. The method is stable and high-efficiency, and is suitable for laboratories, pilot scale tests and industrial production scale-up.

The invention discloses a glucosylglycerol product and a purification method and application of the glucosylglycerol. The method comprises the following steps of 1, obtaining a low-permeability extract rich in GG(glucosylglycerol); 2, carrying out depigmentation treatment by adopting membrane filtration; 3, carrying out depigmentation treatment by adopting resin adsorption; 4, carrying out desalination by adopting membrane filtration; and 5, carrying out dehydration and concentration, specifically, carrying out dehydration on an obtained concentrated solution so as to obtain the purified glucosylglycerol product. The purification method has the advantages of being simple, safe, easy to operate and control, efficient, very suitable for industrial large-scale production and capable of improving the economic benefits.

The invention relates to the technical field of method for manufacturing rotary type sensor used for rapidly, sensitively and specifically detecting trace small RNAs, belongs to the technical field of biosensor, in particular relates to establishment and application of a novel rotary type sensor detection method. The invention has the characteristic that sensitivity and specificity when detecting small RNA with low abundance in cell, tissue, body fluid and blood are rapidly, simply and obviously improved. The invention provides a method for manufacturing novel rotary type small RNAs sensor, used for screening, detecting and comparing expression of endogenous small RNA (such as shRNA, miRNA, piRNA, siRNA and the like) and improving the existing chromatophore purification, preparation and fluorescence labelling, design of probe and sensor assembling and preparation technique of related chip. In the invention, FoF1-ATPase has double actions of biosensor and molecular motor. In the detection process, a probe with high specificity on a molecular motor rotates like a propeller, so that centrifugal force and fluidity of peripheral liquid are improved, thus reducing time for target small RNAs to approach the probe and promoting separation of non-target RNA. Thus, the method can rapidly and simply detect small RNA molecules expressed in cell, tissue, body fluid and blood at low abundance with high sensitivity and high specificity and can be used for identifying and comparing variation of small RNA before and after cell differentiation and diagnosis and evaluation of occurrence and development of disease.

The invention relates to a carbon roasting smoke calcium hydroxide desulfurization and defluorination purification method and device. A preliminary dust remover arranged on an outlet of a roasting furnace is used for removing tar and dust; smoke enters a full-evaporation dry bottom type cooling tower to be subjected to spraying cooling; after cooling, the smoke enters an adsorption reaction separator, the smoke and inner circulation calcium hydroxide adsorption agents are subjected to a primary adsorption reaction, materials collected in the adsorption reaction separator are conveyed to a fluoride-containing stock bin through a conveying device to be stored; the smoke and added fresh calcium hydroxide adsorption agents are subjected to secondary adsorption reaction through a desulfurization and defluorination reactor, and the desulfurization and defluorination process is performed; then the smoke enters a cloth bag dust remover and is subjected to the gas and solid separation filtering process, and gas generated after separation and filtration is discharged from a chimney through an induced draught fan. Sulfur dioxide, fluoride, dust and tar in the smoke are removed while calcium hydroxide is adopted, the purposes of desulfurization, defluorination and dust removal are achieved, and the method and device have the advantages that the technology is reasonable and simple, operation is convenient, equipment is easy to manufacture, cost is low, the floor space is small, maintenance is easy, running is reliable, and service life is long.