609results about How to "Simple purification method" patented technology

The invention discloses a luminescent material of a series of fluorene derivatives. A structure of the material is as shown in formula I. The material as shown in formula I is easily dissolved in an organic solvent, is good in heat stability and can be used to prepare a luminescent device in a way of fluid manufacturing procedures such as printing, instilling, coating and printing. An organic electroluminescent device prepared by coating the material has the characteristics that the power efficiency is good, the material utilization rate is high, the manufacturing cost of an OLED device is greatly decreased, and the material synthesis and purification method is simply suitable for mass production, so that the luminescent material is an ideal choice of the organic electroluminescent device luminescent material. Utilizing the organic electroluminescent diode material as a main body material or a doping material in a luminescent layer or independently as a luminescent material or a hole transmission material or an electron transmission material also falls within a protection scope (see the specification).

The invention discloses a pyrazine derivative and application thereof in an organic electroluminescence device. The structure of the material is as shown in formula I in the specification. By adopting the material, the start voltage of the organic electroluminescence device prepared from the material can be reduced, the current efficiency and the electroluminescence efficiency of the device can be improved, the service life of the device can be prolonged, and moreover, the material has the characteristics of being simple in synthesis and purification method, applicable to large-scale production and the like, and is an ideal option as a material of the organic electroluminescence device. As the light-emitting layer of an OLED (Organic Light-Emitting Diode) device made of the fluorescent material is high in fluorescence efficiency and good in stability, the fluorescence efficiency and the service life of the device can both meet the requirement of practical utilization.

A spiro [fluorene-9, 9-xanthene]-class hole transport material and application thereof are disclosed, the spiro [fluorene-9, 9-xanthene]-class hole transport material is an aromatic-amine compound using spiro [fluorene-9, 9-xanthene] as a core-shell structure, the aromatic-amine compound has one or more than 1 N nuclear structure unit, two adjacent N nuclear structure units are connected by a connecting group, the N nuclear structure unit meets the general formula F, and Rx, Ry and Rz are substitutes or connecting groups. Compared with similar products in the prior art, the spiro [fluorene-9, 9-xanthene]-class hole transport material is the aromatic-amine compound using the spiro [fluorene-9, 9-xanthene] as the core-shell structure, has higher glass transition temperature and thermal decomposition temperature, higher redox potential, and higher hole mobility and conductivity, and has great application value and broad application prospects in the field of perovskite solar cells and other organic electronic devices.

The invention discloses a preparation method of tofacitinib, namely 3-{(3R,4R)-4-methyl-3-[methyl(7H-pyrrolo[2,3-d] pyrimidin-4-yl)amino]piperidin-1-yl}-3-oxopropanenitrile. According to the preparation method, an onium pyridine salt is formed by using benzyl bromide as a raw material; then reduction, selective oxidation, alkylation, deprotection and an acylation reaction are conducted to obtain a compound, namely 3-{(3R,4R)-4-methyl-3-[methyl(7H-pyrrolo[2,3-d] pyrimidin-4-yl)amino]piperidin-1-yl}-3-oxopropanenitrile shown as the formula I.

The invention relates to a method for preparing a sub-nanometer gold cluster molecule. The purification of the prior part is complex. The method of the invention firstly uses chloroauric acid as raw material and adopts a solution method for synthesizing a gold nanometer crystal solution. Then, the gold nanometer crystal solution and amine solution with the molar concentration of 1mM-10M are mixed and react for more than 30min. After the reaction is finished, the centrifugal separation is carried out on the solution to obtain sub-nanometer gold cluster molecular solution. The sub-nanometer gold cluster molecule obtained by the method is monodispersed Au8 cluster molecule. Only common centrifugation is required for separating other impurities. The purification mode is simple.

The invention discloses a novel method used for preparing high-purity 4-chloropyrrolo[2,3-d]pyrimidine in industrialization scale. According to the method, ethyl cyanoacetate, bromo-acetal, formamidine acetate, and sodium alcoholate are taken as raw materials; N,N-dimethylformamide, ethyl alcohol, and phosphorus oxychloride are taken as solvents; and 4-chloropyrrolo[2,3-d]pyrimidine is obtained via three-step approach. The process route of the method is simple; time is short; next step feed can be carried out directly without complex purification process of intermediates; no toxic agent is used; the method is safe and reliable, and is low in cost; total yield is higher than 30%; and the purity of obtained products is as high as 99.2%.

The invention discloses a flucloxacillin sodium compound and a method for preparing the same. The method comprises that: firstly, N'N-dibenzylethylene diamine salt and flucloxacillin acid are mixed and reacted to form a salt; and secondly, high-purity flucloxacillin sodium is obtained through the displacement of a cation exchange resin. The invention adopts a purification method which is simple and easy to operate, thereby greatly improving the purity of the flucloxacillin sodium compound to a certain degree, removing a large amount of high molecular polymers, stabilizing the product quality of related preparations and ensuring the safety of clinic medication use.

The invention provides a preparation method of long-chain alkyl amidopropyl dimethylamine with CO2 responsiveness, and relates to the preparation, purification and structure of several long-chain alkyl amidopropyl dimethylamine compounds with CO2 responsiveness as well as CO2 responsiveness characterization. The long-chain alkyl amidopropyl dimethylamine with CO2 responsiveness comprises palmitylamidopropyl dimethylamine, stearamidopropyl dimethylamine, oleamidopropyl dimethylamine and behenamidopropyl dimethylamine, and is low in preparation temperature, simple in the preparation method and high in the yield; and the aqueous solution of the long-chain alkyl amidopropyl dimethylamine has CO2 responsiveness, and phase transition can happen when CO2 is led in, so that the long-chain alkyl amidopropyl dimethylamine can be applied to reaction and separation with different polarity requirements, and the aqueous solution of the long-chain alkyl amidopropyl dimethylamine can be used as CO2-responsive gel. A compound prepared by the preparation method disclosed by the invention can also be used for increasing the recovery ratio of oil and gas fields.

The invention belongs to the synthesis technical field of organic medicine, and discloses a synthesis method of anti-influenza and avian influenza virus resistant medicine peramivir, which is characterized by adopting (1R, 4S)-2-azabicyclo[2.2.1]hept-5-en-3-one as a raw material and by comprising the following steps: step I: catalyzing, loop-opening and amino protection; step II: 1,3-dipolarcycloaddition; step II: sodium borohydride and nickel chloride hexahydrate reduction and acetylization; and step IV: amino protection removal, and chloroformamidine hydrochloride is adopted as reaction agent to generate guanidyl and hydrolysis methyl ester to prepare peramivir. The sodium borhydride and the nickelous chloride reduction system is used for substituting the expensive platinum dioxide to be used as reducing agent during the hydrogenation reduction process, and the self-produced chloroformamidine hydrochloride is used during the process for feeding the guanidyl reagent, so the cost is reduced, and the operation procedures are simplified. The synthesis method has short reaction routine and moderate reaction condition, is simple and convenient to operate, has low cost, and is suitable for the industrialized production.

The invention discloses a codon-optimized 7 beta-hydroxy steroid dehydrogenase gene, a recombination expression vector Pgex-6P-1-7 beta-HSDH containing an optimized gene and escherichia coli containing the recombination expression vector. Glutathione S-transferase (GST) fusion expression of the codon-optimized 7 beta-hydroxy steroid dehydrogenase gene and a prokaryotic expression vector Pgex-6P-1 in the escherichia coli is adopted, so that a large quantity of active target proteins with an integral structure can be generated in a short time; and a method for purifying the GST fusion expression protein is simple and quick, so that the active target proteins can be quickly obtained.

The invention relates to a method for purifying a high-purity organic solvent-acetone for scientific research, which comprises the following steps that: acetone as the raw material is adsorbed to remove impurities by an adsorption resin, dried to remove water by a drying agent, rectified by a rectifying still, filtered and packaged to obtain a chromatographic grade and pesticide residue grade acetone product. The method can obtain the chromatographic acetone product with the purity more than 99.9 percent, ensure that the yield is more than 93 percent and the qualification rate is more than 95 percent, can obtain a 50 to 60 percent pesticide residue grade acetone reagent, can meet the application demand of high efficiency liquid chromatography (HPLC) customers on pesticide residue, and fill in the gap of the national production of pesticide residue grade chromatography reagents; and at the same time, compared with the prior method, the method has high product purity, simple and convenient operation and stable operation, and is suitable for mass production.

Different from the conventional method for purifying human serum albumin from human plasma, the invention provides a simple method for separating and purifying the human serum albumin from fermentation liquor or a mammal cell culture solution. By an immobilized metal affinity chromatography (IMAC) step, the method is suitable for purifying recombinant proteins at various salt concentrations, fermentation supernate at high salt concentration is allowed to be directly loaded, the total volume of loading buffer is effectively reduced and the cost is reduced. The method is stable and high-efficiency, and is suitable for laboratories, pilot scale tests and industrial production scale-up.

The invention discloses a glucosylglycerol product and a purification method and application of the glucosylglycerol. The method comprises the following steps of 1, obtaining a low-permeability extract rich in GG(glucosylglycerol); 2, carrying out depigmentation treatment by adopting membrane filtration; 3, carrying out depigmentation treatment by adopting resin adsorption; 4, carrying out desalination by adopting membrane filtration; and 5, carrying out dehydration and concentration, specifically, carrying out dehydration on an obtained concentrated solution so as to obtain the purified glucosylglycerol product. The purification method has the advantages of being simple, safe, easy to operate and control, efficient, very suitable for industrial large-scale production and capable of improving the economic benefits.

The invention discloses a method for preparing brivaracetam. The method for preparing brivaracetam comprises the following step that in an organic solvent, under the condition of anhydrous and inert gas shielding, a compound V and L-2-aminobutanamide are subjected to a condensation reaction to obtain brivaracetam I. According to the method for preparing brivaracetam, brivaracetam is prepared through only four steps of reacting, the reaction steps are short, the total yield is high, aftertreatment steps and purifying methods are simple, a product with the de value larger than 99.80% can be prepared only through recrystallization, the grade API is reached, the production cost is low, and the method is suitable for industrial production. The formula is shown in the description.

The invention discloses a coumarin oxime ester photoinitiator and a preparation method thereof. The coumarin oxime ester photoinitiator is obtained by performing an oximation reaction on a coumarin compound containing a formyl group and NH2OH.HCl to obtain an oxime compound, and finally performing an esterification reaction on the oxime compound and acyl chloride. The photoinitiator is capable of initiating a polymerization reaction under visible light enough in intensity, and has the characteristics of high initiation activity and high curing speed.

Novel chimeric nucleic acids, encoding chimeric Borrelia proteins comprising OspC or an antigenic fragment thereof and OspA or an antigenic fragment thereof, are disclosed. Chimeric proteins encoded by the nucleic acid sequences are also disclosed. The chimeric proteins are useful as vaccine immunogens against Lyme borreliosis, as well as for immunodiagnostic reagents.

The invention discloses 7alpha-hydroxyl steroid dehydrogenase gene optimized by a codon, a recombinant expression vector pGEX-6p-1-7alpha-HSDH containing the optimized gene, and escherichia coli containing the recombinant expression vector. The 7alpha-hydroxyl steroid dehydrogenase gene optimized by the codon is used to carry out GST (glutathione S-transferase) fusion expression with a prokaryotic expression vector pGEX-6p-1 in the escherichia coli. A lot of target proteins with activity and intact structure can be generated within a short time, and the purification method of GST fused protein is simple and fast, thereby rapidly obtaining the target protein with activity.

The invention relates to dihydroxyl quaternary ammonium salt with antimicrobial activity as well as a preparation method and application of the dihydroxyl quaternary ammonium salt, belonging to the field of antibacterial materials and preparation methods. The preparation method comprises steps of carrying out reaction on N-R1-diethanol amine and halogenated hydrocarbon (R2-X) in an organic solvent, after precipitation of the precipitator, recrystallizing repeatedly so as to obtain the dihydroxyl quaternary ammonium salt (N-R1-N-R2-N,N-diethoxy ammonium halide). The quaternary ammonium salt is simple to synthesize, has high yield and low cost, has high reaction speed and simple and convenient purification method. The dihydroxyl quaternary ammonium salt has good capacity in resisting both various bacteria such as escherichia coli and various funguses such as physalospora piricala, and has good application prospect.

The invention discloses a method for synthesizing sunitinib, which comprises the following steps that: tert-butyl acetoacetate and acetylacetic ester are taken as initial raw materials, and tetra-substituted pyrrole is obtained through a nitrosification reduction reaction; then 2,4-dimethyl pyrrole-3-formic acid is obtained through the hydrolysis and is amidated; then an aldehyde group is utilized on position 5 of pyrrole through a Vilsmeier-Hacck reaction; and finally the obtained product and 5-fluorooxindole react to obtain the sunitinib. The method has low cost and simple operation, and is favorable for industrial production.

The invention relates to a preparation method of an anilinoquinazoIine compound, in particular to a method for preparing gefitinib and an intermediate thereof. The preparation method of the invention not only avoids the use of highly-pollutant halogenating agents to greatly reduce environmental pollution, but is also characterized by the connection with a 3-halogenated propyl side chain at first prior to the synthesis of a quinazoline parent ring and then introduction of a morpholine ring at the last step of the synthesis, thus repeated adjustment for pH value in the purification of a reaction product of every step is avoided, a purification method is simplified and the yield is raised.

The invention discloses a series of fluorescent OLED materials, which have the structure as shown in a formula I. The materials shown in the formula I have delayed fluorescence property; the organic electroluminescent device prepared by utilizing the materials can obtain deep blue organic light emitting diodes, the noble metal phosphorescent materials can be replaced, the manufacturing cost of the OLED device is greatly lowered, the material synthesis and purification methods are suitable for large-scale production, and the materials are an ideal choice of a luminescent material of the organic electroluminescent device.

The invention relates to the technical field of method for manufacturing rotary type sensor used for rapidly, sensitively and specifically detecting trace small RNAs, belongs to the technical field of biosensor, in particular relates to establishment and application of a novel rotary type sensor detection method. The invention has the characteristic that sensitivity and specificity when detecting small RNA with low abundance in cell, tissue, body fluid and blood are rapidly, simply and obviously improved. The invention provides a method for manufacturing novel rotary type small RNAs sensor, used for screening, detecting and comparing expression of endogenous small RNA (such as shRNA, miRNA, piRNA, siRNA and the like) and improving the existing chromatophore purification, preparation and fluorescence labelling, design of probe and sensor assembling and preparation technique of related chip. In the invention, FoF1-ATPase has double actions of biosensor and molecular motor. In the detection process, a probe with high specificity on a molecular motor rotates like a propeller, so that centrifugal force and fluidity of peripheral liquid are improved, thus reducing time for target small RNAs to approach the probe and promoting separation of non-target RNA. Thus, the method can rapidly and simply detect small RNA molecules expressed in cell, tissue, body fluid and blood at low abundance with high sensitivity and high specificity and can be used for identifying and comparing variation of small RNA before and after cell differentiation and diagnosis and evaluation of occurrence and development of disease.

The invention discloses a tanshinone II A derivative and a preparation method and application thereof. The structural formula of the tanshinone II A derivative is as shown in Formula (I). The preparation method adopts tanshinone II A and aldehydes for synthesis and has the advantages of safe and simple operation and high yield. The tanshinone II A derivative has a function of inhibiting the growth of tumor cells, can be applied in preparation of anti-tumor drugs and has potential value for industrial development and great significance for research and development of anticancer drugs.

The invention discloses a making technique of entikawei monohydrate as antiviral drug, which comprises the following steps: reacting cyclopentadiene monomer and sodium hydride protected by inert gas to make sodium cyclopentadiene; oxidizing through tert-butyl hydroperoxide; reacting with benzyl halogen under alkaline to obtain compound 3 to react with 6-benzyloxy guanine acted by alkaline to obtain compound 4; reacting with amino protective group to prepare compound 5; oxidizing through DMP tert-butanol oxidizing system to obtain ketone compound 6 to generate methylene compound 7 acted by Zn / TiCl4 / CH2Br2; stripping protective product under acid condition to produce compound 8; recrystallizing to obtain the product.

The invention discloses a double-functional fusion protein based on an antibacterial peptide, a preparation method and an application thereof. The double-functional fusion protein based on the antibacterial peptide is a fusion protein based on a cecropins B protein sequence and a human epidermal growth factor; the linking protein is especially designed to be a locus which can be identified by thrombin; and a purified His tag is constructed on the fusion protein. In the invention, the biological preparation for the fusion protein is realized by using a prokaryotic expression system and the purified fusion protein is obtained. A bacteriostasis test and a verification for realizing live animals are performed by the purified fusion protein to indicate that the fusion protein can be applied to preparing a medicine for treating epidermal wound, can be applied to diseases such as epidermal wound, abrade, burn and scald, etc., and can prevent wound from being infected.

A Chinese patent medicine for treating rheumatoid arthritis, relieving inflammation and relaxing pain is prepared from oriental bittersweet stem through extracting and separating.

The invention relates to the technical field of preparation of organic compounds, in particular to the technical field of preparation of a metal-chelator. The invention particularly discloses a preparation method of imino-disuccinic acid and salt thereof. The method comprises the steps of mixing alkaline raw material liquid A containing butene diacid radical and alkaline raw material liquid B containing aspartic acid radical to have a reaction, enabling reaction liquid to be in a critical saturation state, and maintaining the reaction liquid to be boiled; continuously carrying out the reaction until end to obtain imino-disuccinate; continuously feeding inorganic acid into the product to obtain imino-disuccinic acid precipitate, filtering, and recrystallizing to obtain white imino-disuccinic acid crystal. The method has the advantages of being short in reaction time, high in reaction yield, low in energy consumption and low in cost; the prepared product is high in purity and good in performance, thus being suitable for industrial production.

The invention provides a purification method for a silver nanowire. The purification method is characterized by comprising the steps that firstly, the silver nanowire is dispersed in a polyvinyl pyrrolidone (PVP) aqueous solution to obtain silver nanowire dispersion liquid, another kind of solvents is added to agglomerate the silver nanowire, still standing is carried out, and supernatant liquid is removed; and secondly, the first step is repeated three to five times, and the purified silver nanowire is obtained. By means of the method, the purity of the nanowire can be greatly improved. When the transmittance of the purified nanowire is 99.1%, the surface resistance is as low as 130 ohm. The needed raw materials are all common reagents, so that the operation method is simple and convenient. The purification method for the silver nanowire is simple, economical and easy to operate.

The invention relates to a carbon roasting smoke calcium hydroxide desulfurization and defluorination purification method and device. A preliminary dust remover arranged on an outlet of a roasting furnace is used for removing tar and dust; smoke enters a full-evaporation dry bottom type cooling tower to be subjected to spraying cooling; after cooling, the smoke enters an adsorption reaction separator, the smoke and inner circulation calcium hydroxide adsorption agents are subjected to a primary adsorption reaction, materials collected in the adsorption reaction separator are conveyed to a fluoride-containing stock bin through a conveying device to be stored; the smoke and added fresh calcium hydroxide adsorption agents are subjected to secondary adsorption reaction through a desulfurization and defluorination reactor, and the desulfurization and defluorination process is performed; then the smoke enters a cloth bag dust remover and is subjected to the gas and solid separation filtering process, and gas generated after separation and filtration is discharged from a chimney through an induced draught fan. Sulfur dioxide, fluoride, dust and tar in the smoke are removed while calcium hydroxide is adopted, the purposes of desulfurization, defluorination and dust removal are achieved, and the method and device have the advantages that the technology is reasonable and simple, operation is convenient, equipment is easy to manufacture, cost is low, the floor space is small, maintenance is easy, running is reliable, and service life is long.